Journal of Pharmaceutical Research

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The present paper deals with the development and validation of a stability indicating reverse phase HPLC method for the Simultaneous Determination of Perindopril Erbumine and Indapamide on Phenomenex Luna C18 column (250mm × 4.6mm, 10μm). A mobile phase consisting of Acetonitrile : Buffer pH 2.8 (40 : 60 % v/v) was used. The flow rate was 1.2 mL min-1. The separation was performed at room temperature. Detection was carried out at 225 nm by UV detection. Separation was completed within 10 min. The developed method was statistically validated for linearity, accuracy, specificity, Method precision, Intermediate Precision and stability. Calibration curves were linear with correlation coefficient between 0.99 to 1.0 over a concentration range of 70-130% (2.8 to 5.2 μg mL-1) of Perindopril Erbumine and 70-130% (0.88 to 1.63 μg mL-1) for Indapamide respectively. The relative standard deviation (R.S.D) was found <2.0%. This method was successfully used for quantification of Perindopril Erbumine and Indapamide combination in Bulk and tablet formulations.

Keywords

Perindopril Erbumine (PE), Indapamide, RP-HPLC, Stability, Validation, Simultaneous Determination.
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