Journal of Pharmaceutical Research

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.

Fullscreen Fullscreen Off


A simple, sensitive and accurate spectrophotometric method has been developed for the estimation of mycophenolate mofetil in bulk and pharmaceutical formulation. The method is based on the formation of green colored chromogen with 3-methyl-2-benzothiazolinone-hydrazonehydrochloride reagent in the presence of 0.03M ferric chloride and obeys beer's law in the range of 2-10 μg/mL exhibiting the maximum absorbance at 659 nm. The limit of detection and quantification were found to be 0.87 μg/mL and 2.66 μg/ mL respectively. The calibration curve demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient higher than 0.999. The regression equation of the curve is Y=0.082x-0.019. The method is fully validated according to ICH guidelines.

Keywords

Mycophenolate Mofetil, 0.03m Ferric Chloride, 3-Methyl-2-Benzothiazolinone Hydrazonehydrochloride (MBTH) Reagent, Validation, ICH Guidelines.
User
Notifications
Font Size