Synthesis of Pyrazole Derivatives: a New Therapeutic Approach for Antiubercular and Anticancer Activity

In present study (5-(5-chloro-1,3-diphenyl-1H-pyrazole-4-yl)-3-(substitutedphenyl)-1H-pyrazol-1-yl)(pyridine- 4-yl)methanone (4a-4o)were synthesized by starting with acetophenone and phenylhydrazine resulting in the formation of acetophenonephenylhydrazone(1). Compound (1) on Vilsmeier-Haack reaction yielded 5-chloro-1,3-diphenyl-¹H-pyrazole-4-carbaldehyde (2), which on condensation with substituted acetophenone gave substituted arylidenepyrazoles(3a-3o) followed by the reaction with isoniazid yielded the titled compounds (4a-4o). All the newly synthesized pyrazolederivatives were characterized by UV, FTIR, 1H NMR, MASS spectrometry and elemental analysis. All the newly synthesized derivatives were also evaluated for their antitubercular activity against Mycobacterium tuberclosis H₃₇Rv using agar dilution method as well as for anticancer activity against MCF7 (Human breast cancer) cells by SRB assay.