Journal of Pharmaceutical Research

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Development and Validation of RP - HPLC Method for Simultaneous Determination of Dicyclomine and Mefanamic Acid


Affiliations
1 Department of Pharmaceutical Chemistry, Shivnagar Vidya Prasarak Mandal’s College of Pharmacy, Malegaon (Bk.), Tal- Baramati, Dist- Pune- 413115, Maharashtra, India
2 Sinhgad College of Pharmacy, Sy.No. 44/1, Off Sinhgad Road, Vadgaon (Bk.), Pune- 411041, Maharashtra, India
 

Objective: The aim of present work was to develop a RP-HPLC method for simultaneous analysis of Dicyclomine (DCL) and Mefanamic acid (MFA) in a tablet dosage form. Method: Waters Chromatographic system was optimized using a Lichrocart C18 column (250 × 4.60 × 5im) with mobile phase comprising of 50 mM KH2PO4: Acetonitrile in the ratio of 75:25. The flow rate was adjusted to 1.0 ml/min with UV detection at 256 nm. Result: DCL and MFA were eluted with retention times of 7.213±0.3 min and 11.102±0.3 min respectively. Beer's Lambert's Law was obeyed over the concentration ranges of 5-25 ig/ml, 50-250 ig/ml for DCL and MFA respectively. Conclusion: The high recovery and low coefficients of variation confirm the suitability of the method for simultaneous analysis of both drugs in a tablet dosage form. Statistical analysis proves that the method is sensitive and significant for the analysis of DCL and MFA in pure and in pharmaceutical dosage form without any interference from the excipients. The method was validated in accordance with ICH guidelines.

Keywords

Dicyclomine, Mefanamic Acid, Simultaneous Estimation, HPLC.
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  • Development and Validation of RP - HPLC Method for Simultaneous Determination of Dicyclomine and Mefanamic Acid

Abstract Views: 249  |  PDF Views: 98

Authors

Baokar Shrikrishna
Department of Pharmaceutical Chemistry, Shivnagar Vidya Prasarak Mandal’s College of Pharmacy, Malegaon (Bk.), Tal- Baramati, Dist- Pune- 413115, Maharashtra, India
Sugandha Mulgund
Sinhgad College of Pharmacy, Sy.No. 44/1, Off Sinhgad Road, Vadgaon (Bk.), Pune- 411041, Maharashtra, India
Nisharani Ranpise
Sinhgad College of Pharmacy, Sy.No. 44/1, Off Sinhgad Road, Vadgaon (Bk.), Pune- 411041, Maharashtra, India

Abstract


Objective: The aim of present work was to develop a RP-HPLC method for simultaneous analysis of Dicyclomine (DCL) and Mefanamic acid (MFA) in a tablet dosage form. Method: Waters Chromatographic system was optimized using a Lichrocart C18 column (250 × 4.60 × 5im) with mobile phase comprising of 50 mM KH2PO4: Acetonitrile in the ratio of 75:25. The flow rate was adjusted to 1.0 ml/min with UV detection at 256 nm. Result: DCL and MFA were eluted with retention times of 7.213±0.3 min and 11.102±0.3 min respectively. Beer's Lambert's Law was obeyed over the concentration ranges of 5-25 ig/ml, 50-250 ig/ml for DCL and MFA respectively. Conclusion: The high recovery and low coefficients of variation confirm the suitability of the method for simultaneous analysis of both drugs in a tablet dosage form. Statistical analysis proves that the method is sensitive and significant for the analysis of DCL and MFA in pure and in pharmaceutical dosage form without any interference from the excipients. The method was validated in accordance with ICH guidelines.

Keywords


Dicyclomine, Mefanamic Acid, Simultaneous Estimation, HPLC.