Journal of Surface Science and Technology

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Concentration of Capping Agent Controls Size Selection, Agglomeration and Antimicrobial Action of Silver Nanoparticles


Affiliations
1 Department of Microbiology, Institute of Post Graduate Medical Education and Research, 244 Acharya Jagadish Chandra Bose Road, Kolkata – 700020, West Bengal, India
2 Advanced Mechanical and Materials Characterization Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja Subodh Chandra Mullick Road, Kolkata – 700032, West Bengal, India
     

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Polyvinyl Alcohol (PVA) capped Ag nanoparticles (AgNPs) formed through reduction of Silver Nitrate (AgNO3) by Sodium Borohydride (NaBH4) were studied for different PVA concentrations (C, %w/w of solution) in the bulk. Diameter (DAg) of Ag metallic core, obtained from plasmon resonance in Ultraviolet-Visible spectra, had a peak (≈12.8 nm) at C~0.24, which almost exactly matched the gyration sphere diameter of PVA molecule (∼13 nm). Cluster diameter (Dcl), measured after a month through Dynamic Light Scattering (DLS) study, gave the number of NPs per cluster showing a dip at C∼0.24. Minimum inhibitory concentration (MIC) of AgNPs on Escherichia coli (E. coli), assayed from standard broth dilution method (CLSI M07-A8), showed MIC of 0.66 μg/ml at the PVA concentration of 0.24%. Scanning Electron Microscopy (SEM) showed NP clusters accumulating over E. coli to have Dcl ∼ DAg whereas those lying outside the bacteria to have Dcl ∼ 3DAg, consistent with a strong size selectivity of antimicrobial efficacy.

Keywords

Coalescence, Minimum Inhibitory Concentration, Polyvinyl Alcohol, Silver Nanoparticles.
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  • S. Kheybari, N Samadi, S.V. Hosseini, A. Fazeli and M.R. Fazeli. DARU J. Pharma. Sci., 18, 168 (2010).

  • B. Li and T.J. Webster. J Orthop Res., 36, 22 (2018). https://doi.org/10.1002/jor.23656. PMid: 28722231, PMCid: PMC5775060.

  • V. Shriram, T. Khare, R. Bhagwat and R. Shukla, V. Kumar. Front. Microbiol., 9, 2990 (2018). https://doi.org/10.3389 fmicb.2018.02990. PMid: 30619113, PMCid: PMC6295477.

  • K.F. Kong, L. Schneper and K. Mathee. APMIS, 118, 1 (2010). https://doi.org/10.1111/j.1600-0463.2009.02563.x. PMid: 20041868, PMCid: PMC2894812.

  • A.L. Peter. Adv. Drug Deliv. Rev., 57, 1471 (2005). https://doi.org/10.1016/j.addr.2005.04.003. PMid: 15964098.

  • A.L. Lerminiaux and A.D.S. Cameron. Can. J. Microbiol., 65, 34 (2019). https://doi.org/10.1139/cjm-2018-0275. PMid: 30248271.

  • S. Galdiero, S. Mariateresa, M. Vitiello, M. Cantisani, V. Marra and M. Galdiero. Molecules., 16, 8894 (2011). https:/ doi.org/10.3390/molecules16108894. PMid: 22024958, PMCid: PMC6264685.

  • O.V. Khorolskyi. Ukr. J. Phys., 63, 144 (2018). https://doi.org/10.15407/ujpe63.2.144.

  • M.A. Gatoo, S. Naseem, M.Y. Arfat, A.M. Dar, K. Qasim and S. Zubair. Biomed Res Int., 498420, 1 (2014). https://doi.org/10.1155/2014/498420. PMid: 25165707, PMCid: PMC4140132.

  • B.A. Cristina, O. Gherasim, A.M. Grumezescu, L. Mogoantă, A. Ficai and E. Andronescu, Nanomaterials (Basel, Switzerland), 8, 681 (2018). https://doi.org/10.3390/nano8090681. PMid: 30200373, PMCid: PMC6163202.

  • S.K. Das, M.M.R. Khan, T. Parandhaman, F. Laffir, A.K. Guha, G. Sekaranaand and A.B. Mandal. Nanoscale, 5, 5549 (2013). https://doi.org/10.1039/c3nr00856h. PMid: 23680871.

  • A. Mandal, V. Meda, W.J. Zhang, K.M. Farhan and A. Gnanamani. Colloids Surfaces B: Biointerfaces, 90, 191 (2012). https://doi.org/10.1016/j.colsurfb.2011.10.021.

  • A. Mandal, R.S.G. Krishnan, S. Thennarasu, S. Panigrahi and A.B. Mandal. Colloids Surfaces B: Biointerfaces, 79, 136 (2010). https://doi.org/10.1016/j.colsurfb.2010.03.042.

  • B. Naskar, S. Ghosh and S.P. Moulik. Langmuir, 28, 7134 (2012). https://doi.org/10.1021/la3000729. PMid: 22506970.

  • S. Das, A. Bhattacharya, N. Debnath, A. Datta and A. Goswami. Appl. Microbiol. Biotechnol., 97, 6019 (2013). https://doi.org/10.1007/s00253-013-4868-z. PMid: 23588933.

  • L. Wang, C. Hu and L. Shao. Int. J. Nanomedicine., 12, 1227 (2017). https://doi.org/10.2147/IJN.S121956. PMid: 28243086, PMCid: PMC5317269.

  • S. Das, N. Debnath, S. Mitra, A. Datta and A. Goswami. BioMetals., 25, 1009 (2012). https://doi.org/10.1007 s10534-012-9567-1. PMid: 22752843.

  • C.F. Ponce, J.P.N Miranda, D.M. Santos, E. Aguado, F.G. Cozar and R. Litrán. J. Nanopart Res., 20, 305 (2018). https://doi.org/10.1007/s11051-018-4406-0. PMid: 30524191, PMCid: PMC6244783.

  • O. Choi, K.K. Deng, N.J. Kim, L.J. Ross, R.Y. Surampalli and Z. Hu. Water Research, 42, 3066 (2008). https://doi.org/10.1016/j.watres.2008.02.021

  • A. Mandal, S. Sekar, K.M.S. Meera, A. Mukherjee, T.P. Sastry and A.B. Mandal. Physical Chemistry Chemical Physics, 16, 20175 (2014). https://doi.org/10.1039/C4CP02554G. PMid: 25138771.

  • A. Mandal, S. Sekar, M. Kanagavel, N. Chandrasekaran, A. Mukherjee and T.P. Sastry. Biochimical et Biophysical Acta (BBA)-General Subjects, 1830, 4628 (2013). https://doi.org/10.1016/j.bbagen.2013.05.018. PMid: 23707714.

  • A. Mandal, S. Sekar, N. Chandrasekaran, A. Mukherjee and T.P. Sastry. J. Mater. Chem B., 3, 3032 (2015). https://doi.org/10.1039/C4TB02124J. PMid: 32262503.

  • A. Mandal, S. Sekar, N. Chandrasekaran, A. Mukherjee and T.P. Sastry. RSC Advances, 5, 15763 (2015). https://doi.org/10.1039/C4RA09694K.

  • A. Mandal, S. Sekar, N. Chandrasekaran, A. Mukherjee and T.P. Sastry. Proceedings of the Institution of Mechanical Engineers, Part H. Journal of Engineering in Medicine, 227, 1224 (2013). https://doi.org/10.1177/0954411913499290

  • A. Kędziora, M. Speruda, E. Krzyżewska, J. Rybka, A. Łukowiak and G.B. Płoskońska. Int. J. Mol. Sci., 19, 444 (2018). https://doi.org/10.3390/ijms19020444. PMid: 29393866, PMCid: PMC5855666.

  • M. Ghanipour and D. Dorranian. Journal of Nano Medicine, 2013, 897043 (2013). https://doi.org/10.1155/2013/897043.

  • C. Willard. The Effects of K1F bacteriophage on the EV36 strain of E. coli. http://www2.optics.rochester.edu/workgroups/cml/me111/sp98projects/courtney/index.html.

  • B. Lugtenberg. Trends Biochem. Sci., 6, 262 (1981). https:// doi.org/10.1016/0968-0004(81)90095-5.

  • X. Mu and Z. Zhong. Int. J. Pharm., 318, 55 (2006). https://doi.org/10.1016/j.ijpharm.2006.03.016. PMid: 16624507.

  • K.K.Y. Ho, J.W. Lee, D. Gregory, S. Majumdar and A.P. Liu. PLoS ONE., 12, e0174689 (2017). https://doi.org/10.1371/journal.pone.0174689. PMid: 28358875, PMCid: PMC5373588.

  • B. Ravinathan, K. Shunmugavel, S. Subramanian, V.P. Dharmalingam, V. Naranappa, M. Sampath and P. Manavalan. Polym Plast Technol Eng., 55, 889 (2016). https://doi.org/10.1080/03602559.2015.1103263


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  • Concentration of Capping Agent Controls Size Selection, Agglomeration and Antimicrobial Action of Silver Nanoparticles

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Authors

Rehana Parveen
Department of Microbiology, Institute of Post Graduate Medical Education and Research, 244 Acharya Jagadish Chandra Bose Road, Kolkata – 700020, West Bengal, India
Alokmay Datta
Advanced Mechanical and Materials Characterization Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja Subodh Chandra Mullick Road, Kolkata – 700032, West Bengal, India
Prasanta Kumar Maiti
Department of Microbiology, Institute of Post Graduate Medical Education and Research, 244 Acharya Jagadish Chandra Bose Road, Kolkata – 700020, West Bengal, India

Abstract


Polyvinyl Alcohol (PVA) capped Ag nanoparticles (AgNPs) formed through reduction of Silver Nitrate (AgNO3) by Sodium Borohydride (NaBH4) were studied for different PVA concentrations (C, %w/w of solution) in the bulk. Diameter (DAg) of Ag metallic core, obtained from plasmon resonance in Ultraviolet-Visible spectra, had a peak (≈12.8 nm) at C~0.24, which almost exactly matched the gyration sphere diameter of PVA molecule (∼13 nm). Cluster diameter (Dcl), measured after a month through Dynamic Light Scattering (DLS) study, gave the number of NPs per cluster showing a dip at C∼0.24. Minimum inhibitory concentration (MIC) of AgNPs on Escherichia coli (E. coli), assayed from standard broth dilution method (CLSI M07-A8), showed MIC of 0.66 μg/ml at the PVA concentration of 0.24%. Scanning Electron Microscopy (SEM) showed NP clusters accumulating over E. coli to have Dcl ∼ DAg whereas those lying outside the bacteria to have Dcl ∼ 3DAg, consistent with a strong size selectivity of antimicrobial efficacy.

Keywords


Coalescence, Minimum Inhibitory Concentration, Polyvinyl Alcohol, Silver Nanoparticles.

References





DOI: https://doi.org/10.18311/jsst%2F2020%2F24875