Journal of Surface Science and Technology

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Lysozyrne-DPPC Interactions in the Model Membrane, DPPC-H2O


Affiliations
1 Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
2 Biochemistry Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
3 Applied Chemistry Divison, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
4 Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012, India
     

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The effect of the protein, lysozyme, on the hydrocarbon chain melting phase trasition of the model membrane system, DPPC-H2O/D2O, as been investigated by DSC, 1H NMR and fluorescence spectroscopy. It is found that the protein binds to the lipid headgroups and that the effective lipid headgroup-headgroup interaction is increased due to lipid-protein interaction. The tryptophan residue(s) of the proteinis implicated in thisassociation. The interaction isstronger when the membrane is in the form of vesicles rather than that of lamellar stacks. The lipid headgroup-lysozyrne binding results in reduced mobility of the acyl chains as well as the headgroup of the lipid molecule.

Keywords

DSC, 1H NMR, Fluorescence, Model Membrane, Lysozyme, Protein-Lipid Interactions.
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  • Lysozyrne-DPPC Interactions in the Model Membrane, DPPC-H2O

Abstract Views: 381  |  PDF Views: 5

Authors

P. S. Parvathanathan
Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
Geeta Datta
Biochemistry Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
E. B. Mirza
Applied Chemistry Divison, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
Lata Sumanan
Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
K. Usha Deniz
Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085, India
K. V. Ramanathan
Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012, India
S. Raghotama
Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012, India
N. Suyaprakash
Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012, India
C. L. Khetrapal
Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012, India

Abstract


The effect of the protein, lysozyme, on the hydrocarbon chain melting phase trasition of the model membrane system, DPPC-H2O/D2O, as been investigated by DSC, 1H NMR and fluorescence spectroscopy. It is found that the protein binds to the lipid headgroups and that the effective lipid headgroup-headgroup interaction is increased due to lipid-protein interaction. The tryptophan residue(s) of the proteinis implicated in thisassociation. The interaction isstronger when the membrane is in the form of vesicles rather than that of lamellar stacks. The lipid headgroup-lysozyrne binding results in reduced mobility of the acyl chains as well as the headgroup of the lipid molecule.

Keywords


DSC, 1H NMR, Fluorescence, Model Membrane, Lysozyme, Protein-Lipid Interactions.