Journal of Surface Science and Technology

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Interfacial Hydration Studies in Enzyme Hosted Reverse Micelles by Oxygen-17 NMR Relaxation Spectroscopy


Affiliations
1 Department of Chemistry, Hamdard University, New Delhi 110062, India
2 Department of Chemistry, University of Delhi, Delhi 110007, India
     

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Spin-lattice and spin-spin relaxation rates of oxygen-17 of water in aerosol OT reverse micelles hosting hydrophilic enzymes like peroxidase and α-chymotrypsin have been measured. It has been observed that the enzyme show maximum activity when all the water molecules present in the aqueous core are strongly bound to the micellar interface as well as enzyme surface hosted in the micelles leaving no free water domain.

Keywords

Interfacial Hydration,170-NMR, NMR Relaxation, Enzymes in Reverse Micelles.
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  • Interfacial Hydration Studies in Enzyme Hosted Reverse Micelles by Oxygen-17 NMR Relaxation Spectroscopy

Abstract Views: 236  |  PDF Views: 2

Authors

Manoj Varshney
Department of Chemistry, Hamdard University, New Delhi 110062, India
Tanoj Kumar Jain
Department of Chemistry, University of Delhi, Delhi 110007, India
Amarnath Maitra
Department of Chemistry, University of Delhi, Delhi 110007, India

Abstract


Spin-lattice and spin-spin relaxation rates of oxygen-17 of water in aerosol OT reverse micelles hosting hydrophilic enzymes like peroxidase and α-chymotrypsin have been measured. It has been observed that the enzyme show maximum activity when all the water molecules present in the aqueous core are strongly bound to the micellar interface as well as enzyme surface hosted in the micelles leaving no free water domain.

Keywords


Interfacial Hydration,170-NMR, NMR Relaxation, Enzymes in Reverse Micelles.