Journal of Surface Science and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Interaction of Silver Nanoparticles with DNA Building Blocks


Affiliations
1 Chemical Laboratory, Council of Scientific and Industrial Research (CSIR)–Central Leather Research Institute (CLRI), Chennai, 600 020, India
     

   Subscribe/Renew Journal


The interaction of DNA bases and corresponding nucleotides with silver nanoparticles (AgNPs) biofunctionalized by tryptophan, has been investigated by absorption spectroscopy and dynamic light scattering. Aggregation of AgNPs was observed in the presence of ATP and GTP, in contrast to nucleoside. This is due to change in pH of the medium in the presence of triphosphates. Aggregation due to ATP was found to be greater than that of GTP, which is due to the interaction between adeninium ion and tryptophanyl residue on the surface of nanoparticle. There is a direct relationship between ATP concentration and aggregation of AgNPs, which in future might assist in developing new protocols for sensing of adenine nucleotides.

Keywords

Tryptophan, Adenine, ATP, Plasmon Resonance Absorption, Aggregation, Adsorption.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 243

PDF Views: 2




  • Interaction of Silver Nanoparticles with DNA Building Blocks

Abstract Views: 243  |  PDF Views: 2

Authors

Loganathan Bhavani Devi
Chemical Laboratory, Council of Scientific and Industrial Research (CSIR)–Central Leather Research Institute (CLRI), Chennai, 600 020, India
Asit Baran Mandal
Chemical Laboratory, Council of Scientific and Industrial Research (CSIR)–Central Leather Research Institute (CLRI), Chennai, 600 020, India

Abstract


The interaction of DNA bases and corresponding nucleotides with silver nanoparticles (AgNPs) biofunctionalized by tryptophan, has been investigated by absorption spectroscopy and dynamic light scattering. Aggregation of AgNPs was observed in the presence of ATP and GTP, in contrast to nucleoside. This is due to change in pH of the medium in the presence of triphosphates. Aggregation due to ATP was found to be greater than that of GTP, which is due to the interaction between adeninium ion and tryptophanyl residue on the surface of nanoparticle. There is a direct relationship between ATP concentration and aggregation of AgNPs, which in future might assist in developing new protocols for sensing of adenine nucleotides.

Keywords


Tryptophan, Adenine, ATP, Plasmon Resonance Absorption, Aggregation, Adsorption.