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Green Synthesis of Copper Nanoparticles and their Antibacterial Property


Affiliations
1 Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata-700032, India
2 University of Santiago de Compostela, Faculty of Chemistry, Department of Physical Chemistry, Avenida das Ciencias, s/n, 15782 Santiago de Compostela, Spain
3 Department of Chemistry, Sree Chaitanya College, Habra, West Bengal, India
     

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We report the synthesis of copper nanoparticle using a completely green protocol. Aqueous copper sulphate solution was used as a precursor of copper nanoparticle whereas L-ascorbic acid and starch acted as reducing agent and stabilizer respectively. Formation of copper nanoparticle was confirmed by colour, UV-VIS spectroscopy and X-Ray Diffraction (XRD) results. The as-synthesized copper nanoparticles show characteristic plasmonic band at 590 nm. High-Resolution Electron Microscopy (HRTEM) shows almost spherical particles having average diameters of 5.7 ± 1.8 nm. As-synthesized copper nanoparticle exhibits antibacterial activity for both Gm-positive bacteria, Bacilus subtilis and Gm-negetive bacteria, Escherecia coli. Plate count and Minimum Inhibitory Concentration (MIC) studies show higher susceptibility of B. subtilis towards copper nanoparticle

Keywords

Antibacterial Activity, Copper Nanoparticle, Green Synthesis
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  • Green Synthesis of Copper Nanoparticles and their Antibacterial Property

Abstract Views: 390  |  PDF Views: 7

Authors

Gargi Dinda
Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata-700032, India
Dipankar Halder
Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata-700032, India
Carlos Vazquez-Vazquez
University of Santiago de Compostela, Faculty of Chemistry, Department of Physical Chemistry, Avenida das Ciencias, s/n, 15782 Santiago de Compostela, Spain
M. Arturo Lopez-Quintela
University of Santiago de Compostela, Faculty of Chemistry, Department of Physical Chemistry, Avenida das Ciencias, s/n, 15782 Santiago de Compostela, Spain
Atanu Mitra
Department of Chemistry, Sree Chaitanya College, Habra, West Bengal, India

Abstract


We report the synthesis of copper nanoparticle using a completely green protocol. Aqueous copper sulphate solution was used as a precursor of copper nanoparticle whereas L-ascorbic acid and starch acted as reducing agent and stabilizer respectively. Formation of copper nanoparticle was confirmed by colour, UV-VIS spectroscopy and X-Ray Diffraction (XRD) results. The as-synthesized copper nanoparticles show characteristic plasmonic band at 590 nm. High-Resolution Electron Microscopy (HRTEM) shows almost spherical particles having average diameters of 5.7 ± 1.8 nm. As-synthesized copper nanoparticle exhibits antibacterial activity for both Gm-positive bacteria, Bacilus subtilis and Gm-negetive bacteria, Escherecia coli. Plate count and Minimum Inhibitory Concentration (MIC) studies show higher susceptibility of B. subtilis towards copper nanoparticle

Keywords


Antibacterial Activity, Copper Nanoparticle, Green Synthesis

References





DOI: https://doi.org/10.17834/ijsstissst.v31i1-2.79931