- Mrunal Patil
- Balaji Almale
- Jyoti Kasture
- Balbir Singh Shah
- Shital Dharrao
- S. V. Mahajan
- Rajyaguru Devangana
- B. S. Shah
- Amrinder Kaur
- Arjun Chakravarti
- Archana Ukey
- Pradnya Saragade
- Girish Taori
- Sharad Ashok Waghachaure
- Aakash Gupta
- Sheela chikhalikarsheela@gmail.com
- Devangana M. Rajyaguru
- Anupama R. Kolte
- Priyanka Sadashiv Baviskar
- Rajendra A. Chaudhari
- Devangana Rajyaguru
- Gayatri Soneta
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Bajaj, Preeti
- Microteaching in Medical Education
Authors
1 Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik, IN
2 Department of Ophthalmology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik, IN
3 Department of Community Medicine, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik, IN
Source
MVP Journal of Medical Sciences, Vol 1, No 2 (2014), Pagination: 84–86Abstract
Medical teachers need training in pedagogic techniques in order to become better teachers. Despite the fact that our teaching techniques improve gradually over the years as we gain experience and also owing to continuous practice undertaken for different kinds of teaching learning situations; nevertheless educational technology has evolved ways and means for better development of teaching skills even at earlier stages by undertaking some methodical exercises, one of which is Microteaching. Microteaching, an innovative technique of teacher training, helps teachers to improve their teaching skills. It aims at development of competence in teaching skills through the practice of microteaching sessions1. In other words, it teaches teachers how to teach. This is especially important in cases of new faculty implying the budding teachers. Even an experienced teacher can benefit by this technique, particularly for learning some new skills1. The individual may be very sound in his/ her own subject but may not necessarily be a good teacher.Keywords
Medical Education, Microteaching- Gaucher's Disease - A Case Report
Authors
Source
MVP Journal of Medical Sciences, Vol 2, No 2 (2015), Pagination: 130-131Abstract
Gaucher's Disease (GD) is an autosomal recessive systemic lysosomal storage disorder which is characterized by glucocerebroside deposition in cells of the macrophage-monocyte system as a result of a deficiency in lysosomal P-glycosidase (glucocerebrosidase). GD is a rare genetic disorder. It is the most common amongst the lysosomal storage disorders. GD has been categorised into three types based on the presence of central nervous involvement1. Type 1 is a non-neuronopathic form that presents in childhood or early adulthood. Type 2 is acute neuronopathic form that presents in childhood. It progresses rapidly and is fatal. Type 3 is chronic non-neuronopathic form that presents in childhood but is slowly progressive. Here we describe a case of a three and a half year old male child in whom a diagnosis of Gaucher's disease was made based on bone marrow biopsy and later confirmed by glucocerebrosidase levels estimation.Keywords
Gaucher’s Disease, Glucocerebroside.References
- Bohra V, Nair V. Gaucher’s disease. Indian J Endocr Metab. 2011; 15:182-6.
- Patel AL, Shaikh WA, Khobragade AK, Soni HG, Joshi AS, Sahasrabudhe GS, Chole PV. Gaucher’s Disease. The Journal of the Association of Physicians of India. 2009 May; 57:410-1.
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- Nagral A, Mewawalla P, Jagadeesh S, et al. Recombinant Macrophage Targeted Enzyme Replacement Therapy for Gaucher Disease in India. Indian Pediatrics. 2011; 48:779- 84.
- Ash Image Bank. Waldenstrom macroglobulinemia with pseudo-Gaucher cells. Blood. 2010 Nov; 116(18):3388.
- Beutler E, Grabowski GA. Glucosylceramide lipidosis-Gaucher disease. The metabolic and molecular bases of inherited diseases. 8th ed. New York: Mc Graw -Hill; 2001. p. 3635-68.
- Neelaveni N, Anunayi J, Raju YR, et al. Pediatric Non-neuronopathic Gaucher Disease- A Case Report. Sch J Med Case Rep. 2014; 2(2):96-9.
- Mc Eachern KA, Fung J, Komarnitsky S, et al. A specific and potent inhibitor of glucosylceramide synthase for substrate inhibition therapy of Gaucher disease. Molecular Genetics and Metabolism. 2007 Jul; 91(3):259-67.
- Priyani AAH. Gaucher’s Disease: a rare disease with an unusual presentation. Journal of Diagnostic Pathology. 2014; 9(1):33-6.
- A Case of Pleomorphic Variant of Embryonal Rhabdomyosarcoma in Skeletal Muscle - A Rare Case Report
Authors
Source
MVP Journal of Medical Sciences, Vol 3, No 2 (2016), Pagination: 122-124Abstract
Embryonal Rhabdomyosarcoma (ERMS) is a malignant soft tissue tumour with phenotypical and biological features of embryonic skeletal muscle cells. It is rare in skeletal musculature of extremities and its pleomorphic variant is rare which is associated with worse outcome. Here we are discussing a case of three year old male child who has presented with recurrence of swelling in right gluteal region arising in gluteus maximus muscle who is a known case of Rhabdomyosarcoma (RMS) 1 year back at the same site. Patient was operated with wide local excision and underwent chemotherapy cycles.Keywords
Embryonal, Pleomorphic, Rhabdomyosarcoma (RMS)References
- Kumar V, Abbas AK. Aster JC. Bones, joints and soft tissue tumors. Robbins and Cotran Pathologic Basis of Disease. 9th ed. 2005. p. 1222–3.
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- Weiss SW, Goldblum JR, Rhabdomyosarcoma. Enzinger and Weiss Soft Tissue Tumours. 5thed. 2008. p. 596–632.
- Kodef RN, William AJr, Hamoudi, et al. Childhood rhabdomyosarcoma with Anaplastic (Pleomorphic) features: A report of intergroup rhabdomyosarcoma Study. The American Journal of Surgical Pathology. May 1993; 17(5).
- Rosai J. MD soft tissues. Rosai and Ackerman’s Surgical Pathology. 10th ed. 2012. p. 2166–70.
- David MP, Dale AE. Rhabdomyosarcoma in adult and children: An update. Archives of Pathology and Laboratory Medicine. 2006 Oct; 130(10):1454–65.
- Mary AF, Julie CF-S. Pleomorphic rhabdomyosarcoma in children: Four cases in paediatric age group. Ann Diagnostic Pathology. 2001 Aug; 5(4):199–206.
- Hyperbasophilic Variant of Acute Promyelocytic Leukemia
Authors
Source
MVP Journal of Medical Sciences, Vol 3, No 2 (2016), Pagination: 125-127Abstract
Acute Promyelocytic Leukemia (APL) is an extremely rare variant of acute myeloid leukemia. APL constitutes around 10-15 % of acute myeloid leukemia in adults. It is commonly diagnosed around 40 years age. Molecular/genetic studies exhibit chromosomal translocation between chromosome 15 and chromosome 17-t(15;17)(q22;q21) and PML-RARa rearrangement. Four variants of APL have been identified: The classic form M3 hypergranular variant, the microgranular variant, the hyperbasophilic form and zinc-finger form-M3r, identified by a different chromosomal translocation, between chromosome 11 and chromosome 17:t(11,17) (q23, q11-12).Keywords
Acute Promyelocytic Leukemia, ATRA, PML/RARαReferences
- Diana C, Vladareanu, et al. Acute promyelocytic leukemia microgranular variant- A clinical and therapeutical approach. Maedica- A Journal of Clinical Medicine. 2007; 2(4):327-331.
- Haslindawani WM, Haslina, et al. A case of hyperbasophilic form of acute promyelocytic leukemia. Malaysian Journal of Medical Sciences. 2008 Jan; Supplement:168.
- Soignet S, Maslak P. Acute promyelocytic leukemia. Wintrobe’s Clinical Hematology. 12th ed. USA: Lipincot Williams and Wilkins, Wolter- Kluwer Business; 2009. p. 1938– 55.
- Elizabeth J, Amita R, Mamta S, et al. An uncommon morphological variant of acute promyelocytic leukemia. American Journal of Hematology. 2011; 87(3):294. Available from: http://wileyonlinelibrary.com/cgi-bin/jhome/35105
- Mohamed M, Dun K, Grabek J. A typical features in a patient with acute promyelocytic leukemia: A potential diagnostic pitfall. BMJ Case Rep. 2013. DOI: 101136/bcr-2013- 200152.
- Ibrahim FA, Yassin MA, El-Ayoubi HR, Alhiji IA, Albinali AS, Almansour SM, Qafoud FM. Clinico-pathological profile of acute promyelocytic leukaemia at Al-Amal Oncology-Haematology Centre, Qatar. East Mediterr Health J. 2010 Sep; 16(9):958–65. PubMed PMID: 21218723.
- A Study of Hematological Profile in Patients of Chronic Renal Failure Undergoing Hemodialysis at a Tertiary Health Care Institute
Authors
1 Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik-422003, Maharashtra, IN
2 Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik-422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 4, No 2 (2017), Pagination: 107-112Abstract
The complete blood count, peripheral blood smear, bleeding time, clotting time and renal function tests of 114 patients of chronic renal failure who had required hemodialysis for a period of at least 3 months prior to the commencement of this study were studied. The purpose of this study was to investigate the derangements in the haematological profile of chronic renal patients undergoing hemodialysis and to correlate the same with the duration and severity of the renal failure. The principal finding in the study was a 100% prevalence of anemia among chronic renal failure patients, which was predominantly of the normocytic normochromic type. Red blood cell count was reduced in nearly all (93%) of study patients. There was also a strong negative correlation (r = -0.74) between haemoglobin and duration of hemodialysis. However, a significant proportion of cases showed abnormal cells in the peripheral blood cells such as burr cells, schistocytes and pencil cells, suggesting other contributing factors to the anemia. Female patients also showed a significantly greater prevalence of increased red cell distribution width (>15%) as compared to males (p = 0.001) and a lower prevalence of normocytic normochromic anemia as compared to males (p = 0.009). White blood cell counts, platelet counts, and bleeding and clotting times were largely within normal limits, and none of these variables showed a statistically significant association with the duration of hemodialysis, or serum creatinine, or serum blood urea nitrogen. The inference from the study is that anemia is a major comorbidity in end-stage renal failure patients, with many factors contributing to it, and detailed workup and effective treatment of anemia is necessary in this group of patients.Keywords
Anemia, Bleeding Time, Clotting Time, Complete Blood Count, Hemodialysis, Normocytic Normochromic.References
- Jha V, et al. Current status of end stage renal disease core in India. Kidney International Supplements. 2013; 3:157–60.https://doi.org/10.1038/kisup.2013.3
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- Rajapurkar, et al. What do we know about chronic kidney disease in India: First report of the Indian CKD registry. BMC Nephrology. 2012; 13:10. https://doi.org/10.1186/14712369-13-10 PMid:22390203 PMCid:PMC3350459
- McGonigle RJ, Shadduck RK, Fisher JW. Erythropoietin deficiency and inhibition of erythropoiesis in renal insufficiency. Kidney Int. 1984; 25:437–44. https://doi.org/10.1038/ ki.1984.36 PMid:6727139
- Subhan-ud-din, Shah SAR. Haemostatic defects in chronic kidney disease. J Med Sci. 2013 Jul; 21(3):149–52.
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- Arun S, Prabhu MV, Chowta KN, Bengre ML. The hematological pattern of the patients with chronic kidney disease in a tertiary care setup in South India. Journal of Clinical and Diagnostic Research. 2012 Aug; 6(6):1003–6.
- Chinwuba I, Uchenna I, Ngozi I. High prevalence of anemia in predialysis patients in Enugu, Nigeria. Nephrology Reviews.2010; 2:14.
- Islam MN, FerdousA, Zahid AZ, Alam M, Islam MN. Haematological profile of patients with chronic kidney disease in Northern Bangladesh. Dinajpur Med Col J. 2015 Jan; 8(1):21–7.
- Bhattacharjee K, Das D, Rabha P, Kalwar AK, Kar G, Bhattacharjee P. A study on hematological profile in patients of chronic renal failure with special reference to serum iron profile. Journal of Evidence based Medicine and Healthcare.2015; 2(46):8212–9. https://doi.org/10.18410/jebmh/ 2015/1107
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- Bhatta S, Aryal G, Kafle RK. Anemia in chronic kidney disease patients in predialysis and postdialysis stages. Journal of Pathology of Nepal. 2011; 1:26–9. https://doi.org/10.3126/ jpn.v1i1.4446
- Barde R, Patel HV, Shah PR. A study of anemia prevalence in CKD patients on maintenance hemodialysis: A single centre study. Journal of Evidence Based Medicine and Healthcare. 2015; 2(39):6344–48. https://doi.org/10.18410/ jebmh/2015/871
- Hakim YAH, et al. The effect of hemodialyis on hemoglobinconcentration Platelet count and white blood cell count
- in end-stage renal failure. Int Journal of Medical Research and Health Sciences. 2016; 5(5):22–35.
- Mudiyammanavara NR, Dhananjaya PE, Agarwal R. Cross sectional study of anaemia in chronic kidney disease. Indian Journal of Basic and Applied Medical Research. 2015 Mar; 4(2):414–9.
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- Tennankore KK, Soroka SD, West KA, Kiberd BA. Macrocytosis may be associated with mortality in chronic hemodialysis patients: A prospective study. BMC Nephrology. 2011; 12:19. https://doi.org/10.1186/1471-2369-12-19 PMid:21569355 PMCid:PMC3114714
- Talwar VK, Gupta HL, Shashinarayan. Clinico-haematological profile in chronic renal failure. The Journal of Association of Physicians of India. 2002; 50:228–33. PMid:12038654 20. Chandra M. Pathogenesis of the anemia of chronic renal failure: The role of erythropoietin. Nefrologia. 1990; 10.
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- Ramaprabha P, Bhuvaneswari T, Kumar RA. Coagulation profiles an indicator of vascular haemostatic function in chronic renal failure patients who are on renal dialysis. Sch J App Med Sci. 2014; 2(2B):592–5.
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- https://doi.org/10.1016/0002-9343(94)90138-4
- Comparison of Bone Marrow Aspiration Cytology, Touch Imprint Cytology and Bone Marrow Biopsy for Bone Marrow Evaluation at a Tertiary Health Care Institute
Authors
1 PG Resident, Department of Pathology, MVP’s Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
2 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
3 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 6, No 2 (2019), Pagination: 152-157Abstract
Introduction: Bone marrow examination is an important investigation in haematology which involves Bone Marrow Aspiration (BMA), Bone Marrow Imprint (BMI) and Bone Marrow Biopsy (BMB). Aim: Correlation of findings of bone marrow aspiration and imprint smears with biopsy in hematological disorders. Materials and Methods: It was a prospective study to correlate bone marrow examination findings by BMA, BMI and BMB of 111 patients in which bone marrow biopsy was done and correlated with aspirate and imprint smears. For aspiration Salah’s needle and for biopsy Jamshidi needle were used. Correlation of hematological and histopathological findings was done. Results: Megaloblastic anaemia was the commonest diagnosis followed by acute leukemia, nutritional anaemia, chronic leukemia, multiple myeloma, leukemia/lymphoma syndrome, and essential thrombocythemia. In case of dry aspirate or diluted marrow biopsy was useful to diagnose aplastic anemia, and secondary myelofibrosis associated with acute and chronic leukemia, multiple myeloma, leukemia/lymphoma syndrome and myelomonocytic leukemia. Aspirate and imprint smears were better for cellular morphology. Conclusion: Aspiration and imprint smears give better morphological details/cytologic diagnosis. In dry tap or diluted marrow, biopsy was more helpful in identifying architectural pattern, cellularity and fibrosis whereas imprint smears were more helpful for cellular morphology. BMA, BMI and BMB are complimentary to each other.Keywords
Anaemia, Bone Marrow Aspiration (BMA), Bone Marrow Imprint (BMI) and Bone Marrow Biopsy (BMB), Haematology, Leukemia.References
- Bain BJ, Clark DM, Wilkins BS. The normal bone marrow. In: Bain BJ, Clark DM, Wilkins BS, editors. Bone Marrow Pathology. 4th ed. Singapore: Wiley-Blackwell; 2010. p. 1-51.
- Bain BJ. Bone marrow trephine biopsy. J. Clin. Pathol. Oct 2001; 54(10):737-42. https://doi.org/10.1136/jcp.54.10.737. PMid: 11577117, PMCid: PMC1731298.
- Bearden JD, Ratkin GA, Colyman. Comparison of the diagnostic value of bone marrow biopsy and aspiration in neoplastic disease. J. Clin. Pathol. 1974; 27:738-40. https://doi.org/10.1136/jcp.27.9.738. PMid: 4426982, PMCid: PMC475459.
- Toi PCh, Varghese RG, Rai R. Comparative evaluation of simultaneous bone marrow aspiration and bone marrow biopsy: An institutional experience. Indian J. Hematol. Blood Transfus. 2010; 26:41-44.
- Sabharwal BD, Malhotra V, Aruna S, Grewal R. Comparative evaluation of bone marrow aspirate particle smears, imprints and biopsy sections. J. Postgrad Med. 1990; 36(4):194-98.
- Antonino Musolino, Annamaria Guazzi, Rita Nizzoli, Michele Panebianco, Cristina Mancini and Andrea Ardizzoni. Accuracy and relative value of bone marrow aspiration in the detection of lymphoid infiltration in non-Hodgkin lymphoma. Tumori. 2010; 96:24-27. https://doi.org/10.1177/030089161009600104. PMid: 20437853.
- Hamid GA, Hanbala N. Comparison of bone marrow aspiration and bone marrow biopsy in neoplastic diseases. Gulf J. Oncology. Jul 2009; (6):41-44.
- Mahajan V, Kaushal V, Thakur S, Kaushik R. A comparativestudy of bone marrow aspiration and bone marrow biopsy in haematological and non haematological disorders - An institutional experience. JIACM. 2013; 14(2):133-35. https://doi.org/10.7788/figurationen.2013.14.2.133.
- Tripathy S, Dudani S. Comparative evaluation of simultaneous bone marrow aspiration and trephine biopsy-Experience from routine clinical practice. Ind. J. Clin. Pract. 2013; 24(5):446-50.
- Bain BJ. Bone marrow trephine biopsy. J. Clin. Pathol. 2001 Sep; 54(9):657-63. https://doi.org/10.1136/jcp.54.9.657. PMid: 11533068, PMCid: PMC1731527.
- Kaur M, Rana A, Kapoor S, Puri A. Diagnostic value of bone [6] marrow aspiration and biopsy in routine haematological practice. J. Clin. Diagn. Res. 2014; 8(8):13-16.
- Smita Chandra, Harish Chandra. Comparison of bone marrow BMA cytology, BMI cytology and BMB for bone marrow evaluation. Hematology Reports. 2011; 3:e22.
- Aboul-Nasr R, Estey EH, Kantarjian HM, Freireich EJ, Andreeff M, Johnson BJ, Albitar M. Comparison of touch imprints with BMA smears for evaluating bone marrow specimens. American Journal of Clinical Pathology. 1999; 111(6):753-58. https://doi.org/10.1093/ajcp/111.6.753. PMid: 10361510.
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- Spectrum of Non Neoplastic Skin Diseases:A Histopathology Based Clinicopathological Correlation Study at a Tertiary Health Care Centre
Authors
1 Professor & Head, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
2 3rd year Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
3 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 6, No 2 (2019), Pagination: 225-230Abstract
Introduction: Skin biopsy probably is the most important ancillary aid to confirm clinical diagnosis. The interpretation of many skin biopsies requires the identification and integration of two different morphological features – the tissue reaction pattern and the pattern of inflammation. Aim: To correlate histopathological diagnosis with clinical diagnosis in various non neoplastic skin lesions. Materials and Methods: The present study was a prospective and observational type of study. A total number of 197 participants were included after satisfying the eligibility criteria with due permission from Department of Dermatology. Only those patients who had given valid informed consent were included in the study. Results: Out of 197 biopsies studied, histopathological diagnosis in 167 biopsies (84.8%) was consistent with clinical diagnosis, while in 30 biopsies (15.2%) histopathological diagnosis was not consistent with clinical diagnosis. Conclusion: Out of 197 cases (M=111, F=86) biopsies studied, histopathological diagnosis was consistent with clinical diagnosis in 167 biopsies (84.8%), while in 30 biopsies (15.2%) the histopathological diagnosis was not consistent with clinical diagnosis. The skin biopsy remains the gold standard for diagnosis which can be supported with other techniques to confirm the diagnosis. This emphasizes the significance of histopathology in diagnosing non neoplastic skin disorders.Keywords
Histopathology, Clinicopathological Correlation, Non Neoplastic Skin Lesions.References
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- Werner B. Skin biopsy and its histopathologic analysis: Why? What for? How? Part I. An Bras Dermatol. 2009; 84(4) : 391–5. https://doi.org/10.1590/S0365-05962009000400010.
- Elder DE, Murphy GF, Elinitsas R, Johnson BL, Xu X. Introduction To Dermatopathologic Diagnosis. Lever’s Histopathology of the Skin. 10th ed.
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- the skin. Indian J Dermatol 2010; 55:225–8. https://doi.org/10.4103/0019-5154.70666.
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- Goyal N, Jain P, Malik R, Koshti A. Spectrum of non neoplastic skin diseases: a histopathology based clinicopathological correlation study. Sch J App Med Sci 2015; 3(1F): 444–9.
- Narang S, Jain R. An Evaluation of Histopathological findings of skin Biopsies in various skin disorders. APALM. 2015; 2(1): A42–46.
- Rajput JS, Singh K, Singh S. Clinicopathological study of nonneoplastic skin disorder. Med Plus Int Med J 2014; 1(8): 367–72.
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- Grover S, Agale SV, D’costa GF, Valand AG, Gupta VK. Clinico-Histopathological Spectrum of Infectious Granulomatous Dermatoses in Western India-A Representative Study from Mumbai. J Clin Diagn Res. 2016; 10(4): 10–14. https://doi.org/10.7860/JCDR/2016/16459.7568.
- Yap FBB. Dermatopathology of 400 skin biopsies from Sarawak. Indian J Derm, Venr, Lepr, 2009; 75(5): 518–519. https://doi.org/10.4103/0378-6323.55407.
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- Spectrum of Hemoglobinopathies Diagnosed by High Performance Liquid Chromatography at a Tertiary Care Centre: An Observational Study
Authors
1 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
2 Former PG Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 7, No 2 (2020), Pagination: 240-245Abstract
Introduction: Thalassemia and other hemoglobinopathies are the most common routinely encountered congenital anemias in India. Sickle cell anemia is very common, so a study to rule out all the hemoglobinopathies is essential. Aims and Objectives: The present study was conducted to diagnose various hemoglobinopathies among clinically suspected cases., the present study was conducted to diagnose various hemoglobinopathies among clinically suspected cases. Materials and Methods: A total of 212 cases cases were undertaken in this study after they met the inclusion and exclusion criteria. HPLC was performed on the samples received in the Central Clinical Laboratory of Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik, Maharashtra. Results: Out of 212 cases, 42 cases showed positivity for thalassemia or presence of abnormal hemoglobin, with 8% cases of Beta thalassemia trait, 1.88% cases of Beta thalassemia major, 4.7% cases of Sickle cell trait, 4.24% cases of Sickle cell disease and 0.94% cases of Sickle-thalassemia. Conclusions: HPLC is a fast, reliable, and cost-effective method to diagnose any suspected case of hemoglobinopathy.
Keywords
HPLC, Thalassemia, HemoglobinopathiesReferences
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- Baruah MK, Saikia M, Baruah A. Pettern of hemoglobinopathies and thalassemias in upper assam region of North East India: High Performance Liquid Chromatography studies in 9000 patients. Indian J Pathol Microbiol. 2014; 57(2):236–43. https://doi.org/10.4103/0377-4929.134680. PMid:24943756
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- Safety of Donor and Recipient in COVID-19 Pandemic
Authors
1 Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
2 Assistant Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
3 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 7, No 2 (2020), Pagination: 282-287Abstract
Novel corona virus is a new strain of corona virus family, caused by SARS-CoV-2, probably of bat origin, not previously identified in humans. The time between entry of virus in body and start of first symptoms is the incubation period of the disease. For COVID-19 the incubation period is from two days to fourteen days. COVID-19 disease spreads through small droplets from nose and mouth. Spread is also through skin contacts and even through air while speaking. Blood is an integral part of the healthcare system. During the COVID-19 pandemic blood transfusion services were affected worldwide. The blood transfusion service had to face many challenges also in India. Voluntary blood donation was decreased which is the main source of blood and blood components. In lockdown, problems of transport services, less staff, fear of getting infected all these factors affected the blood donation services. This pandemic has influenced the blood donation activities causing shortage of blood and blood components and thus affected the blood inventory. Hence the blood services had to make appropriate plans to meet the requirements of patients.Keywords
Blood Transfusion Services, Blood Inventory, Corona Virus, Pandemic, Voluntary Blood Donation, COVID-19References
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- Coronavirus disease 2019 (COVID-19). Mayo Foundation for Medical Education and Research (MFMER). Available from: https://www.mayoclinic.org/diseases-conditions/ coronavirus/symptoms-causes/syc-20479963?p=1.
- Das SS, Biswas RN, Zaman RU. An insight into the encountered challenges and performances of a hospital based blood transfusion service in Eastern India in the light of COVID-19 pandemic. Global Journal of Transfusion Medicine. 2020 Jul 1; 5(2):163.
- Ngo A, Masel D, Cahill C, Blumberg N, Refaai MA. Blood banking and transfusion medicine challenges during the COVID-19 pandemic. Clinics in laboratory medicine. 2020 Dec 1; 40(4):587-601. https://doi.org/10.1016/j. cll.2020.08.013. PMid:33121624 PMCid:PMC7414314.
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- Politis C, Papadaki M, Politi L, Kourti G, Richardson C, Asariotou M, Tsakris A, Mentis A. Post-donation information and haemovigilance reporting for COVID-19 in Greece: Information supporting the absence of SARS-CoV-2 possible transmission through blood components. Transfusion Clinique et Biologique. 2020 Oct 20. https:// doi.org/10.1016/j.tracli.2020.10.007.
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- Arcot PJ, Kumar K, Mukhopadhyay T, Subramanian A. Potential challenges faced by blood bank services during COVID-19 pandemic and their mitigative measures: The Indian scenario. Transfusion and Apheresis Science. 2020 Jul 16:102877. https://doi.org/10.1016/j.transci.2020.102877. PMid:32709475 PMCid:PMC7365118.
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- Sharma HB, Vanapalli KR, Cheela VS, Ranjan VP, Jaglan AK, Dubey B, Goel S, Bhattacharya J. Challenges, opportunities, and innovations for effective solid waste management during and post COVID-19 pandemic. Resources, conservation and recycling. 2020 Nov 1; 162:105052. https://doi.org/10.1016/j.resconrec.2020.105052. PMid:32834486 PMCid:PMC7362850.
- Sapkota A, Poudel S, Sedhain A, Khatiwada N. Blood transfusion practice among healthcare personnel in Nepal: An observational study. Journal of Blood Transfusion. 2018. https://doi.org/10.1155/2018/6190859. PMid:29670804 PMCid:PMC5833242
- A Study of Correlation between Type 2 Diabetes Mellitus and Glycosylated Haemoglobin in a Tertiary Care Centre
Authors
1 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
2 Former PG Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
3 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, IN
Source
MVP Journal of Medical Sciences, Vol 8, No 2 (2021), Pagination: 157-162Abstract
Background: Diabetes is a silent killer. Looking at the alarming presence of diabetes and its morbidity and mortality in India, we need to diagnose this metabolic disorder accurately and at the earliest. We have carried out this study to determine correlation of fasting blood glucose, post meal blood glucose and glycosylated haemoglobin in type 2 Diabetes Mellitus and to find the cut off value of glycosylated haemoglobin to diagnose type 2 diabetes mellitus. Materials and Methods: A cross sectional study was carried out among 298 cases of type 2 Diabetes Mellitus attending a tertiary care centre in Maharashtra during August 2018 to August 2020. Results and Conclusions: Majority were males and in the age group of 41 to 50 years. Fasting Blood Glucose (FBS) and Postprandial Blood Glucose (PPBS) are strongly correlated to Glycosylated Haemoglobin (HbA1c). Association between FBS and PPBS is statistically significant. Correlation of PPBS and HbA1c is stronger than that of FBS and HbA1c. Cut off level of HbA1c is higher in the study subjects in comparison to standard cut off value of 6.5%.Keywords
Fasting Blood Glucose, Glycosylated Haemoglobin, Postprandial Blood Glucose, Type 2 Diabetes MellitusReferences
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- World Health Organization. Diabetes key facts. 2020.
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- American Diabetes Association. Diabetes. 2020. https:// www.diabetes.org/a1c/diagnosis
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- Sherwani SI, Khan HA, Ekhzaimy A, Masood A, Sakharkar MK. Significance of HbA1c Test in diagnosis and prognosis of diabetic patients. Biomarker Insights. 2016: 11:95–104. PMid: 27398023 PMCid: PMC4933534. https:// doi.org/10.4137/BMI.S38440
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- Application of Bethesda System For Cervical Cytology at a Tertiary Care Centre
Authors
1 Professor & Head, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik - 422203, Maharashtra, IN
2 Former PG Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik - 422203, Maharashtra, IN
3 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik - 422203, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 8, No 2 (2021), Pagination: 171-178Abstract
Background: For early screening of the cervical lesion cervical smear is a sensitive test and most widely used system for describing Pap smear result is The Bethesda System. Aims and Objectives: The present study was conducted to look for the epithelial cell abnormalities in cervix using Bethesda system of reporting for cervical cytology, to implicate The Bethesda System of reporting (2014) during assessment of Pap smear, to detect cervical cancer and its pre-cancerous lesions (LSIL-Low grade intraepithelial lesion and HSIL-High grade intraepithelial lesion) at an early stage by Pap smear examination and to create awareness about cervical cancer and importance of Pap smear as a screening test in disease prevention. Materials and Methods: The present study was undertaken at a tertiary health care, out of 250 cervical smears, we excluded 10 smears which were inadequate to opine. So, the total of 240 smears got reported according to The Bethesda System 2014. Observations and Results: The youngest patient was 18 year old and the oldest was 75 year old female. A total of 4% Pap smears were reported as unsatisfactory. The most common complaint of patients was discharge per vaginum which accounts for a total of 38% followed by abdominal pain is showed in 8% of cases. The most common infection was bacterial vaginosis followed by candida, Trichomonas. The frequency of normal (NILM) cases was 78%. ASCUS was found in 4.58%, LSIL was found in 9.17%, HSIL was found in 1.67% & SCC was found in 3.33% cases. Proportion of LSIL was more in age group 41-50 years i.e. 21.15%. Conclusion: Pap test has significant utility worldwide. Bethesda system which is widely used for cervicalcytology is a uniform and standard method for reporting cervical smears. It also gives a descriptive diagnosis that aids the clinicians regarding individualized management of patients. It helps in prevention of invasive cervical cancer by timely detection and treating women having precancerous lesions. The effectiveness is determined by reduction in incidence and mortality.Keywords
Bethesda System, Cervical Cancer, Infection, Pap Smear, Cervical CytologyReferences
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- Solomon D, Davey D, Kurman R et al. The 2001 Bethesda System Terminology for Reporting Results of Cervical Cytology. JAMA. 2002; 287(16):2114–2119
- Denny L, Kuhn L, Pollack A, Wainwright H, Wright Jr TC. Evaluation of alternative methods of cervical cancer screening for resource-poor settings. Cancer: Interdisciplinary International Journal of the American Cancer Society. 2000; 89(4):826-833
- Torre LA, Islami F, Siegel RL, Ward EM, Jemal A. Global Cancer in Women. Burden and Trends. 2017; 26(4):444457.
- Lopez MS, Baker ES, Maza M et al. Cervical cancer prevention and treatment in Latin America. Journal of Surgical Oncology. 2017; 115(5):615-618.
- Cutts FT, Franceschi S, Goldie S, Castellsague XD, De Sanjose S, Garnett G, Edmunds WJ, Claeys P, Goldenthal KL, Harper DM, Markowitz L. Human papillomavirus and HPV vaccines: A review. Bulletin of the World Health Organization. 2007; 85:719-726.
- Sachan PL, Singh M, Patel ML, Sachan R. A study on cervical cancer screening using pap smear test and clinical correlation. Asia Pac J Oncol Nurs. 2018; 5:337-341.
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- Castle PE, Sideri M, Jeronimo J, Solomon D, Schiffman M.
- Risk assessment to guide the prevention of cervical cancer [serial online]. Am J Obstet Gynecol. 2007; 197:356.e1-356.
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- Bal MS, Goyal R, Suri AK, Mohi MK. Detection of abnormal cervical cytology in Papanicolaou smears.
- Journal of Cytology/Indian Academy of Cytologists. 2012 Jan; 29(1):45.
- Nayar R, Wilbur DC, editors. The Bethesda system for reporting cervical cytology: Definitions, criteria, and explanatory notes. Springer. 2015 Apr 13.
- Roghaei MA, Afshar MN, Pooladkhan SH, Roghaie SH.
- Adequacy criteria and cytomorphological changes in liquiprep TM versus conventional cervical cytology. 2010.
- Verma I, JaIn V, Kaur T. Application of bethesda system for cervical cytology in unhealthy cervix. Journal of Clinical and Diagnostic Research: JCDR. 2014 Sep; 8(9):OC26.
- Sankaranarayanan R, Basu P, Wesley RS, Mahe C, Keita N, Mbalawa CC, Sharma R, Dolo Aaltaf, Shastri SS, Nacoulma M, Nayama M. Accuracy of visual screening for cervical neoplasia: Results from an IARC multicentre study in India and Africa. International Journal of Cancer. 2004 Jul 20; 110(6):907-913.
- Vaghela BK, Vaghela VK, Santwani PM. Analysis of abnormal cervical cytology in papanicolaou smears at tertiary care center–A retrospective study. IJBAR. 2014; 5:47-49.
- Sreedevi A, Javed R, Dinesh A. Epidemiology of cervical cancer with special focus on India. International Journal of Women’s Health. 2015; 7:405.
- Altaf FJ, Mufti ST. Pattern of cervical smear abnormalities using the revised Bethesda system in a tertiary care hospital in Western Saudi Arabia. Saudi Med J. 2012 Jun 1; 33(6):634-639.
- Bukhari MH, Saba K, Qamar S, Majeed MM, Niazi S, Naeem S. Clinicopathological importance of Papanicolaou smears for the diagnosis of premalignant and malignant lesions of the cervix. Journal of Cytology/Indian Academy of Cytologists. 2012 Jan; 29(1):20.
- Estimation of Thrombocytopenia in Patients of COVID-19 in a Tertiary Care Centre as a Prognostic marker
Authors
1 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
2 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
3 PG Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
4 Former PG Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik – 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 8, No 2 (2021), Pagination: 191-198Abstract
Introduction: Severe COVID-19 can lead to critical illness, with Acute Respiratory Distress (ARDS) and Multi-organ Failure (MOF) as its primary complications, eventually followed by intravascular coagulopathy. Haematological changes are common in patients with COVID-19, which include reduced lymphocyte count and platelet count but normal white blood cell count and prolonged activated partial thromboplastin time. Using a simple test like platelet count for assessing the risk of mortality and early identification of severe cases will help in preventing the life threatening complications in patients of COVID-19. Aims and Objectives: To study trends of thrombocytopenia in patients of COVID-19 and to study the correlation between thrombocytopenia and severity of cases of COVID-19. Materials and Methods: The study was carried out in Central Clinical Laboratory in a tertiary care centre. A total of 138 random subjects who were admitted in the COVID ICU were included after they satisfied the eligibility criteria. The CBCs were analyzed on the Beckmann Coulter automated cell count analyzer with EDTA samples obtained from peripheral venipuncture of the patients. Platelet trends over the three samples were studied. Results: An average of all three platelets counts for the patients revealed an overall decreasing trend in cases of non survivors, whereas an overall upward trend was noted in the survivors. A total of 79 patients showed thrombocytopenia (platelet count less than 1.5 lakhs/mm3 ), during at least one of the tests.46 (33.33%) of these patients succumbed, whereas 33 (23.9%) patients survived. Decreasing trends or overall decreasing trends (Increasing then decreasing) were observed in larger number of non survivors as compared to survivors. Also increasing or overall increasing trends (decreasing then increasing) were common in the survivors. Discussion: Hematological changes are common in SARS patients. For thrombocytopenia, the possible mechanisms of SARS-CoV associated thrombocytopenia may include, 1. Direct infection of megakaryocytes and platelets potentially, inducing cell apoptosis and growth inhibition and/or 2. Immune damage of megakaryocyte progenitor cells or platelets; In addition, the lung damage in SARS patients may also play a role in inducing thrombocytopenia. Conclusion: In this study, we found that platelet count may be a simple, economic, rapid and commonly available laboratory parameter that could straightforwardly discriminate between COVID patients with and without severe disease, while the study of serial platelets counts as trends could help identifying those with a serious risk of mortality.Keywords
COVID-19, Platelet Trends, Prognostic Marker, ThrombocytopeniaReferences
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