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GC-MS Analysis of Antibacterial Metabolites Extracted from Rhodococcus rhodochrous Isolated from Soil Samples


Affiliations
1 Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, Iran, Islamic Republic of
2 Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Islamic Republic of
3 Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazerun, Iran, Islamic Republic of
 

The genus Rhodococcus is a Gram positive and non motile bacterium with different capability. Nowadays scientists are using the organism for the production of their antimetabolite products. Therefore, in the present study, the genus was isolated from the soil of different parts around the manganese mine in Qom city, Iran. The isolates were characterized using biochemical and antimicrobial test. Antimicrobial properties were evaluated using pathogenic bacteria, including: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC8739), Pseudomonas aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC14028), Shigella dysenteriae (PTCC 1188), Klebsiella pneumophila (ATCC 700603), Candida albicans (ATCC 10231), Bacillus subtilis (PTCC1715), Bacillus cereus (PTCC 1015), Listeria monocytogenes (PTCC1295), Enterococcus faecalis (ATCC51299), Rhodococcus erythropolis (PTCC1767), Corynebacterium glutamicum (PTCC 1532). Furthermore, antibacterial compounds from culture supernatant were extracted using different solvents and the extracts were analysed using GC-MS. The results indicated that 9 of the isolates correctly belonged to the genus Rhodococcus. In addition, the results exhibited potential antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC8739), with average zone inhibition of 8 and 25 mm respectively, but it had no effect on the other organisms. On the other hand the chromatogram showed nine special peaks and the identified compounds were: cyclopenthasiloxane, azulene, 2,7 octadiene, docosane, cyclohexasiloxane, bis (3,5,5 trimethyhexyl, tetraedacane-2 methyl, cycloheptasiloxane and heptadecanol. Phylogenetic analysis showed that the isolated strain from soil around manganese mine in Qom city, Iran formed a distinct subclade within the genus Rhodococcus with R. rhodochrous.

Keywords

Rhodococcus rhodochrous, Antimicrobial Metabolites, GC-MS Analysis, Phylogenetic Analysis.
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  • GC-MS Analysis of Antibacterial Metabolites Extracted from Rhodococcus rhodochrous Isolated from Soil Samples

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Authors

Soheil Aghaei
Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, Iran, Islamic Republic of
N. Bahador
Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Islamic Republic of
M. Baserisalehi
Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazerun, Iran, Islamic Republic of

Abstract


The genus Rhodococcus is a Gram positive and non motile bacterium with different capability. Nowadays scientists are using the organism for the production of their antimetabolite products. Therefore, in the present study, the genus was isolated from the soil of different parts around the manganese mine in Qom city, Iran. The isolates were characterized using biochemical and antimicrobial test. Antimicrobial properties were evaluated using pathogenic bacteria, including: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC8739), Pseudomonas aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC14028), Shigella dysenteriae (PTCC 1188), Klebsiella pneumophila (ATCC 700603), Candida albicans (ATCC 10231), Bacillus subtilis (PTCC1715), Bacillus cereus (PTCC 1015), Listeria monocytogenes (PTCC1295), Enterococcus faecalis (ATCC51299), Rhodococcus erythropolis (PTCC1767), Corynebacterium glutamicum (PTCC 1532). Furthermore, antibacterial compounds from culture supernatant were extracted using different solvents and the extracts were analysed using GC-MS. The results indicated that 9 of the isolates correctly belonged to the genus Rhodococcus. In addition, the results exhibited potential antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC8739), with average zone inhibition of 8 and 25 mm respectively, but it had no effect on the other organisms. On the other hand the chromatogram showed nine special peaks and the identified compounds were: cyclopenthasiloxane, azulene, 2,7 octadiene, docosane, cyclohexasiloxane, bis (3,5,5 trimethyhexyl, tetraedacane-2 methyl, cycloheptasiloxane and heptadecanol. Phylogenetic analysis showed that the isolated strain from soil around manganese mine in Qom city, Iran formed a distinct subclade within the genus Rhodococcus with R. rhodochrous.

Keywords


Rhodococcus rhodochrous, Antimicrobial Metabolites, GC-MS Analysis, Phylogenetic Analysis.