Comparative Decolourisation of Rbbr Dye and Guaiacol Degradation by Free and Immobilized Laccase Producing Bacillus Spp.

The aim of the research work was decolourisation of Remazol Brilliant Blue R (an anthraquinone dye) and guaiacol by free cells and immobilized strains of Bacillus sp. D1023, D1032 and D1033 which were isolated from field soil of Ambala College of Engineering and Applied Research. Comparison between free cells and immobilized cells was observed using 100 mg/L of Remazol Brilliant Blue R and efficiency of beads was observed by reutilization of beads using same concentration of dye. The selected strains D1023, D1032 and D1033 were able to decolourise RBBR dye up to 81.23%, 73.14% and 75.44% by free cells and 94.54%, 84.45% and 89.50% by immobilized cells respectively. These strains are also utilized to treat Guaiacol and corresponding colour change has been observed for free and immobilized strains. To increase the decolourisation process 2×SG medium was used which increases the spore production in culture.