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Effect of Drought Stress on Chlorophyll Content and Anti-Oxidant Enzymes of Green Gram Genotypes (Vigna radiata L.)


Affiliations
1 Department of Crop Physiology, College of Agriculture, PJTSAU, Telanagana, Hyderabad-30, India
2 ANGRAU, Bapatla-522101, Andra Pradesh, India
3 Indian Institute of Maize Research, Rajendranagar, Hyderabad-30, India
 

Green gram (Vigna radiata L.) Wilczek, chiefly grown as a post rainy season crop, faces water stress situation. A complex response, in terms of biochemical and molecular level is shown by plants, when exposed to drought, and depending on that, plants show differential adaptation and tolerance mechanisms. In the studied biochemical parameters, proline, catalase and peroxidase showed increased activity due to water stress and negatively correlated with seed yield and correlation was significant for catalase and peroxidase, while the total chlorophyll content decreased due to water stress and it was positively and significantly correlated with the seed yield. All the biochemical parameters recorded higher values in genotype WGG 37, whereas, lowest total chlorophyll and leaf proline were recorded in MGG 348 and lowest catalase and peroxidase activity in MGG 347. Highest seed yield was recorded by the genotype WGG 37 (1058.71 kg ha-1), followed by WGG 42 (1052.22 kg ha-1), while the lowest seed yield was recorded in MGG 348 (951.42 kg ha-1). Thus indicating the role of the biochemical parameters and total chlorophyll content in stress mitigation.

Keywords

Green Gram, Total Chlorophyll, Proline, Catalase.
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  • Bates, L.S., Waldren, R.P. and Teare, I.D. 1973. Rapid determination of free proline in water stress studies. Plant and Soil, 39: 205-208.

  • Dutta, Bera and Puspendu, A.K. 2007. Oxidative stress and the activity of antioxidant enzymes in mungbean seedling subjected to water stress. Indian J. of Plant Physiology, 12(2): 199-202.

  • Hiscox and Israeltam 1979. A method for extraction of chlorophyll from leaf tissue without maceration. Canadian J. of Botany, 57: 1332-1334.

  • Lalinia, A.A., Majnon Hoseini, N., Galostian, M., Esmaeilzadeh Bahabadi, S. and Marefatzadeh Khameneh, M. 2012. Echophysiological impact of water stress on growth and development of mungbean. Int. J. of Agro. and Plant Prod., 3(12): 599-607.

  • Lowenstein, G. and Linsey, N. 1961. Peroxidase activity in virus infected sweet potatoes. Phytopathology, 51: 63-68.

  • Moradi, A., Ahmadi, A. and Hosseinzadeh, A. 2008. Agro-physiological response of mungbean (cv. Partov) to severe and moderate drought stress applied at vegetative and reproductive growth stages. J. of Sci. and Tech. of Agric. and Natural Resources, 12(45(B)): 659-672.

  • Nair, A.S., Abraham, T.K. and Jaya, D.S. 2008. Studies on the changes in lipid peroxidation and antioxidants in drought stress induced cowpea (Vigna unguiculata L.) varieties. J. of Environ. Bio., 29(5): 689-691.

  • Sonali, P., Shivani, R. and Chakraborty, D. 2014. Analysis of biochemical responses in Vigna mungo varieties subjected to drought stress and possible amelioration. Int. J. of Sci. Res. in Agric. Sci., 1(1): 6-15.

  • Teranishi, Y., Tanaka, A., Osumi, M. and Fukui, S. 1974. Catalase activity of hydrocarbon utilizing candida yeast. Agric. and Bio. Chemistry, 38: 1213-1216.


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  • Effect of Drought Stress on Chlorophyll Content and Anti-Oxidant Enzymes of Green Gram Genotypes (Vigna radiata L.)

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Authors

B. Rambabu
Department of Crop Physiology, College of Agriculture, PJTSAU, Telanagana, Hyderabad-30, India
V. Padma
ANGRAU, Bapatla-522101, Andra Pradesh, India
Ramesh Thatikunta
Department of Crop Physiology, College of Agriculture, PJTSAU, Telanagana, Hyderabad-30, India
N. Sunil
Indian Institute of Maize Research, Rajendranagar, Hyderabad-30, India

Abstract


Green gram (Vigna radiata L.) Wilczek, chiefly grown as a post rainy season crop, faces water stress situation. A complex response, in terms of biochemical and molecular level is shown by plants, when exposed to drought, and depending on that, plants show differential adaptation and tolerance mechanisms. In the studied biochemical parameters, proline, catalase and peroxidase showed increased activity due to water stress and negatively correlated with seed yield and correlation was significant for catalase and peroxidase, while the total chlorophyll content decreased due to water stress and it was positively and significantly correlated with the seed yield. All the biochemical parameters recorded higher values in genotype WGG 37, whereas, lowest total chlorophyll and leaf proline were recorded in MGG 348 and lowest catalase and peroxidase activity in MGG 347. Highest seed yield was recorded by the genotype WGG 37 (1058.71 kg ha-1), followed by WGG 42 (1052.22 kg ha-1), while the lowest seed yield was recorded in MGG 348 (951.42 kg ha-1). Thus indicating the role of the biochemical parameters and total chlorophyll content in stress mitigation.

Keywords


Green Gram, Total Chlorophyll, Proline, Catalase.

References