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Ligninolytic Enzymes Production and Decolorization Potential of Native Fungi Isolated from Pulp and Paper Mill Sludge


Affiliations
1 Department of Environmental Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, India
 

In this study, potential of 30 fungal strains isolated from pulp and paper mill sludge were assessed as biolignolytic microorganisms and their ability to produce ligninolytic enzymes viz., laccase (Lcc), lignin peroxidase (LiP), manganese peroxidase (MnP), xylanase and cellulase using different chromogenic substrates. A total of eight fungi exhibited conspicuous halos with their respective substrate. The fungal isolates were found to be significantly different in qualitative enzyme production. Maximum enzyme activity for Lcc, MnP and LiP were 26.24, 8.5 and 12.99 U/mL exhibited by fungal isolates Trametes sp. (LDFD5), Nigrospora sp. (LDF00204) and Alternaria sp (LDFD4) on 10th and 13th day, respectively, by solid state fermentation (SSF). Conspicuous reduction in colour unit upto 75.29 % and 76.37 % were observed by fungal strain LDF00204 at 30°C temperature and pH 5 respectively. Results depicted that fungal isolate Nigrospora sp. LDF00204 (Accession No. KP732542) exhibited a relatively noble characteristic of having ligninolytic enzymes, is able to degrade lignin and its cocompounds efficiently, and thus it could be utilized for decolorization of pulp and paper mill effluent and also for bioremediation of alike pollutants.

Keywords

Lignin Degrading Fungi, Ligninolytic Enzymes, Black Liquor, Decolorization.
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  • Akhan, J.C. 2008. Physicochemical determination of pollutants in waste water and vegetable samples along the Jakara wastewater Channelin Kano Metropolis, Kano State, Niigeria. Europian Journal scientific Research, 23: 122-23.

  • Alves da Cunha, M.A., Barbosa, A.M., Giese, E.C. and Dekker, R.F.H. 2003. The effect of carbohydrate carbon sources on the production of constitutive and inducible laccases by Botryosphaeria sp. J. Basic Microbiol., 43: 385-392.

  • Bajpai, P., Mehna, A. and Bajpai, P. 1993. Decolorization of kraft bleach plant effluent with the white rot fungus Trametes versicolor. Process Biochem., 28(6): 377-384.

  • Barlev, S.S. and Kirk, T.K. 1981. Effects of molecular oxygen on lignin degradation by Phanerochaete chrysosporium. Biochem. Biophys. Res. Commun., 99(2): 373-378.

  • Bourbonnais, R. and Paice, M.G. 1990. Oxidation of non-phenolic substrates. An expanded role for laccase in lignin biodegradation. FEBS Lett., 267: 99-102.

  • Couto, S.R. and Sanroman, M.A. 2005. Application of solid-state fermentation to ligninolytic enzyme production. J. Biochem. Eng., 22: 211-219.

  • CPCB, 2001. Comprehensive industry document for large pulp and paper industry. COINDS/36/2000-2001.

  • DaRe, V. and Papinutti, L. 2011. Black liquor decolorization by selected white-rot fungi. Appl. Biochem Biotechnol., 165(2): 406-415.

  • Dhouib, A., Hamza, M., Zouari, H., Mechichi, T., Hmidi, R., Labat, M., Martinez, M.J. and Sayadi, S. 2005. Screening for ligninolytic enzyme production by diverse fungi from Tunisia. World J. Microb. Tech., 21: 1514-1523.

  • Haddadin, M.S., Al-Natour, R., Al-Qsous, S. and Robinson, R.K. 2002. Biodegradation of lignin in olive pomace by freshly isolated species of basidiomycete. Bioresour. Technol., 82: 131-137.

  • Ilyas, S. and Rehman, A. 2013. Decolorization and detoxification of Synozol red HF-6BN azo dye by Aspergillus niger and Nigrospora sp. Iranian J. Environ. Health Sci. & Eng., 10(1): 12.

  • Lara, M.A., Rodrguez-Malaver, A.J., Rojas, O.J., Holmquist, O., Gonzalez, A.M., Bullon, J., Penaloza, N. and Araujo, E. 2003. Black liquor lignin biodegradation by Trametes elegans. Int. J. Biodeterior. Biodegrad., 52: 167-173.

  • Linger, J.G., Vardon, D.R., Guarnieri, M.T., Karp, E.M., Hunsinger, G.B., Franden, M.A., Johnson, C.W., Chupka, G., Strathmann, T.J., Pienkos, P.T. and Beckham, G.T. 2014. Lignin valorization through integrated biological funneling and chemical catalysis. Proceedings of National Academy of Sciences, 1-6.

  • Lorenzo, M., Moldes, D. and Rodriguez Couto, S., and Sanroman, A. 2002. Improvement in laccase production by employing different lignocellulosic wastes in submerged cultures of Trametes versicolor. Bioresour. Technol., 82: 109-113.

  • Makris, S.P. and Banerjee, S. 2002. Fate of resin acids in pulp mills secondary treatment systems. Water Research, 36: 2878-82.

  • Nerud, F. and Misurcova, Z. 1996. Distribution of ligninolytic enzymes in selected white rot fungi. Folia Microbiol., 41: 264-266.

  • Owens, J.W., Swanson, S.M. and Birkholz, D. 1994. Environmental monitoring of bleached kraft pulp mill chlorophenolic compounds in a Northern Canadian River System. Chemosphere, 29(1): 89-109.

  • Pahkala, K., Mela, T., Hakkola, H., Järvi, A. and Virkajärvi, P. 1996. Production and use of agrofibre in Finland. Final report of the study, Part I. Production of agrofibre crops: Agronomy and varieties. Agricultural Research Centre of Finland, Jokioinen, Finland, pp. 84.

  • Pant, D. and Adholeya, A. 2006. Isolation and screening of potential fungi for decolorization of distillery wastewaters. In: A. MendezVilas (Ed.), Modern Multidisciplinary Applied Microbiology Exploiting Microbes and their Interactions, Weinheim: Wiley. pp. 95-102.

  • Pawle, G. and Singh, S.K. 2014. Antimicrobial, antioxidant activity and phytochemical analysis of an endophytic species of Nigrospora isolated from living fossil Ginkgo biloba. Curr. Res. Environ. & Appl. Mycol., 4(1): 1-9.

  • Pointing, S.B. 1999. Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi. Fungal Divers., 2: 17-33.

  • Pukahuta, C., Suwanarit, P., Shinagawa, E., Hoshida, H. and Nishizawa, Y. 2004. Combination of laccase, xylanase, and cellulase in lignocellulose degradation by white rot fungi, Lentinus polychrous Lev. and L. squarrosulus Mont. Kasetsart Journal (Natural Science), 38: 65-73.

  • Raghukumar, C. and Rivonkar, G. 2001. Decolorization of molasses spent wash by the white-rot fungus Flavodon flavus, isolated from a marine habitat. Appl. Microbiol. Biotechnol., 55: 510-514.

  • Revankar, M.S. and Lele, S.S. 2006. Enhanced production of laccase using a new isolate of white rot fungus WR-1. Process Biochem., 41: 581-588.

  • Sharari, M., Jahan Latibari, A., Guillet, A., Aurousseau, M., Mouhamadou, B., Rafeiee, G., Mirshokraei, A. and Parsapaghouh, D. 2011. Application of the white rot fungus Phanerochaete chrysosporium in biotreatment of bagasse effluent. Biodegradation, 22: 421-430.

  • Silva, I.S., Grossman, M. and Durrant, L.R. 2009. Degradation of polycyclic aromatic hydrocarbons (2-7 rings) under microaerobic and very-low-oxygen conditions by soil fungi. Int. Biodeterior. Biodegrad., 63: 224-229.

  • Singh, D. and Chen, S. 2008. The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes. Appl. Microbiol. Biotechnol., 81: 399-417.

  • Singhania, R.R., Patel, A.K., Soccol, C.R. and Pandey, A. 2009. Recent advances in solid-state fermentation. Biochem. J. Biochem. Eng., 44: 13-18.

  • Tien, M. and Kirk, T.K. 1984. Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proceedings of the National Academy of Sciences USA, 81(8): 2280-2284.

  • Tien, M. and Kirk, T.K. 1988. Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol., 161: 238-248.

  • Tong, P., Hong, Y., Xiao, Y., Zhang, M., Tu, X. and Cui, T. 2007. High production of laccase by a new basidiomycete, Trametes sp. Biotechnol. Lett., 29: 295-301.

  • Vaithanomsat, P., Apiwatanapiwat, W., Petchoy, O. and Chedchant, J. 2010. Production of ligninolytic enzymes by white-rot fungus Datronia sp. KAPI0039 and their application for reactive dye removal. Int. J. Chem. Eng., 162(504): 1-6.

  • Wariishi, H., Valli, K. and Gold, M. 1992. Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: Kinetic mechanism and role of chelators. J. Biol. Chem., 267: 23688-23695.

  • WHO 2007. Dioxins and their effects on human health. Resource document. World health organization. http://www.who.int/ mediacentre/factsheets/fs225/en/.

  • Wu, J., Xiao, Y.Z. and Wang, Y.P. 2002. Treatment of pulp mill wastewaters by white rot fungi. J. Biol., 19: 17-19.

  • Wu, J., Xiao, Y.Z. and Yu, H.Q. 2005. Degradation of lignin in pulp mill wastewaters by white rot fungi on biofilm. Bioresour. Technol., 96: 1357-1363.

  • Zhang, M., Wu, F., Wei, Z.Y., Xiao, Y.Z. and Gong, W.M. 2006. Characterization and decolorization ability of a laccase from Panus radis. Enz. Microb. Technol., 39: 92-97.


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  • Ligninolytic Enzymes Production and Decolorization Potential of Native Fungi Isolated from Pulp and Paper Mill Sludge

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Authors

Deepika Rajwar
Department of Environmental Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, India
Sarita Joshi
Department of Environmental Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, India
J. P. N. Rai
Department of Environmental Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, India

Abstract


In this study, potential of 30 fungal strains isolated from pulp and paper mill sludge were assessed as biolignolytic microorganisms and their ability to produce ligninolytic enzymes viz., laccase (Lcc), lignin peroxidase (LiP), manganese peroxidase (MnP), xylanase and cellulase using different chromogenic substrates. A total of eight fungi exhibited conspicuous halos with their respective substrate. The fungal isolates were found to be significantly different in qualitative enzyme production. Maximum enzyme activity for Lcc, MnP and LiP were 26.24, 8.5 and 12.99 U/mL exhibited by fungal isolates Trametes sp. (LDFD5), Nigrospora sp. (LDF00204) and Alternaria sp (LDFD4) on 10th and 13th day, respectively, by solid state fermentation (SSF). Conspicuous reduction in colour unit upto 75.29 % and 76.37 % were observed by fungal strain LDF00204 at 30°C temperature and pH 5 respectively. Results depicted that fungal isolate Nigrospora sp. LDF00204 (Accession No. KP732542) exhibited a relatively noble characteristic of having ligninolytic enzymes, is able to degrade lignin and its cocompounds efficiently, and thus it could be utilized for decolorization of pulp and paper mill effluent and also for bioremediation of alike pollutants.

Keywords


Lignin Degrading Fungi, Ligninolytic Enzymes, Black Liquor, Decolorization.

References