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Diversity, Identification and Biotyping of Bacillus thuringiensis Strains from Soil Samples in Iran


Affiliations
1 Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Islamic Republic of
2 Immunology Department, Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Islamic Republic of
3 Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Islamic Republic of
 

Bacillus thuringiensis is a bacterium known for producing insecticidal and cytocidal crystal protein during sporulation. One hundred soil samples were analysed which were introduced by seven methods, out of which sixty B. thuringiensis strains were collected to study the distribution and diversity of crystal proteins. The strains were characterized on the basis of microscopic examination of parasporal crystal morphology, biochemical tests, and analysis of parasporal inclusions protein profiles by SDS-PAGE. Majority of strains had bipyramidal crystals. B. thuringiensis subsp. thuringiensis was the most common biochemical type. Overall, according to our finding, it could be concluded that genetic diversity of B. thuringiensis strains is dependant on the geographical areas. It means that the production of crystal protein by different strains of B. thuringiensis probably dependant on the geographical area.

Keywords

Bacillus thuringiensis, Biochemical Type, Crystal Protein.
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  • Diversity, Identification and Biotyping of Bacillus thuringiensis Strains from Soil Samples in Iran

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Authors

Elham Moazamian
Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Islamic Republic of
Nima Bahador
Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Islamic Republic of
Manoochehr Rasouli
Immunology Department, Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Islamic Republic of
Negar Azarpira
Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Islamic Republic of

Abstract


Bacillus thuringiensis is a bacterium known for producing insecticidal and cytocidal crystal protein during sporulation. One hundred soil samples were analysed which were introduced by seven methods, out of which sixty B. thuringiensis strains were collected to study the distribution and diversity of crystal proteins. The strains were characterized on the basis of microscopic examination of parasporal crystal morphology, biochemical tests, and analysis of parasporal inclusions protein profiles by SDS-PAGE. Majority of strains had bipyramidal crystals. B. thuringiensis subsp. thuringiensis was the most common biochemical type. Overall, according to our finding, it could be concluded that genetic diversity of B. thuringiensis strains is dependant on the geographical areas. It means that the production of crystal protein by different strains of B. thuringiensis probably dependant on the geographical area.

Keywords


Bacillus thuringiensis, Biochemical Type, Crystal Protein.

References