Enrichment of Lipid Accumulation in Chlorella pyrenoidosa

In this study, the microalgae Chlorella pyrenoidosa was subjected for growth with respect to supply different heterotrophic carbon sources such as glucose, sucrose and fructose, also autotrophic condition with CO₂ as the carbon source are considered in various concentrations such as 0%, 10% and 20%. The maximum biomass concentration of 7.3 g/L was obtained with glucose as the carbon source and the lipid productivity was said to be higher with 20% of CO₂ concentration with lipid content of 22.13%. This study concludes that the lipid content in the algal cells was increased at 20% of CO₂ as the carbon source. This indicates that the lipid content can be increased at the autotrophic growth condition mediated by the supply of CO₂ to the medium as the carbon source compared with that of the other heterotrophic level of carbon supplementary. The optimization of the algal growth has been performed at different growth conditions along with that of the different stress parameters together in order to understand and monitor the relation between the cell components of algae such as lipid content, protein content and chlorophyll content and their effect with that of the applied stress conditions.