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A Complete Study on Biodegradation of Reactive Red C2G29 Using Newly Isolated Acinetobacter sp


Affiliations
1 Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode, India
2 Department of Biotechnology, Madha Engineering College, Kunrathur, Chennai, India
3 Department of Microbiology, Periyar University, Salem, India
     

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A bacterium designated as Acinetobacter sp., isolated from domestic sewage soil identified by taxonomic studies by 16S rDNA sequential analysis was found to decolorize azo dye with the decolorization efficiency varying from 80-96%. This strain decolorized 96.2% a deep red sulphonated azo dye Reactive RedC2G29 (200 mg/L) with 1.76 g/L dry cells of specific decolorization rate in static condition at optimum pH 5.0 and temperature 30°C. The degradation efficiency of this strain was within 48 h. Phytotoxicity studies revealed less toxic nature of decolorized products as compared to original dye. HPLC, GC-MS and LC-MS analysis revealed the production of metabolites as final product by degradation of the dye.

Keywords

Reactive Red C2G29, Degradation, Acinetobacter sp.
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  • A Complete Study on Biodegradation of Reactive Red C2G29 Using Newly Isolated Acinetobacter sp

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Authors

D. Suresh
Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode, India
P. Sridhar
Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode, India
D. Joyce Hellen Sathya
Department of Biotechnology, Madha Engineering College, Kunrathur, Chennai, India
M. Anil Kumar
Department of Biotechnology, Madha Engineering College, Kunrathur, Chennai, India
M. Seenuvasan
Department of Biotechnology, Madha Engineering College, Kunrathur, Chennai, India
R. Dhandapani
Department of Microbiology, Periyar University, Salem, India

Abstract


A bacterium designated as Acinetobacter sp., isolated from domestic sewage soil identified by taxonomic studies by 16S rDNA sequential analysis was found to decolorize azo dye with the decolorization efficiency varying from 80-96%. This strain decolorized 96.2% a deep red sulphonated azo dye Reactive RedC2G29 (200 mg/L) with 1.76 g/L dry cells of specific decolorization rate in static condition at optimum pH 5.0 and temperature 30°C. The degradation efficiency of this strain was within 48 h. Phytotoxicity studies revealed less toxic nature of decolorized products as compared to original dye. HPLC, GC-MS and LC-MS analysis revealed the production of metabolites as final product by degradation of the dye.

Keywords


Reactive Red C2G29, Degradation, Acinetobacter sp.