Research Journal of Engineering and Technology

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Statistical Optimization of Lipase Production from Bacillus spp by Central Composite Design


Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai 600069, India
2 Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai 600025, India
     

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A Response Surface approach has been used to study the production of an extracellular lipase from Bacillus species. This research paper mainly focused on developing a media component and physical parameters for lipase production by Central Composite Design (CCD).Interaction were studied for four different factors are olive oil concentration, ammonium sulphate concentration, pH and Temperature. By using the statistical approach (Response Surface Methodology) the optimum enzyme activity of 8.69 IU/ml was predicted when olive oil concentration 1.5%, ammonium sulphate concentration 0.4%, pH 8, Temperature 40ºC.

Keywords

Central Composite Design, Lipase Activity, Maximum Yield, Response Surface Methodology, Statistical Approach.
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  • Statistical Optimization of Lipase Production from Bacillus spp by Central Composite Design

Abstract Views: 145  |  PDF Views: 1

Authors

R. Saranya
Department of Biotechnology, Madha Engineering College, Chennai 600069, India
K. Tamilarasan
Department of Biotechnology, Madha Engineering College, Chennai 600069, India
N. Peatciyammal
Department of Biotechnology, Madha Engineering College, Chennai 600069, India
Rajamaniccam L. Sai
Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai 600025, India
M. Dharmendira Kumar
Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai 600025, India

Abstract


A Response Surface approach has been used to study the production of an extracellular lipase from Bacillus species. This research paper mainly focused on developing a media component and physical parameters for lipase production by Central Composite Design (CCD).Interaction were studied for four different factors are olive oil concentration, ammonium sulphate concentration, pH and Temperature. By using the statistical approach (Response Surface Methodology) the optimum enzyme activity of 8.69 IU/ml was predicted when olive oil concentration 1.5%, ammonium sulphate concentration 0.4%, pH 8, Temperature 40ºC.

Keywords


Central Composite Design, Lipase Activity, Maximum Yield, Response Surface Methodology, Statistical Approach.