Research Journal of Engineering and Technology

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Optimization of Amylase Production from Aspergillus oryzae MTCC 1847 by Submerged Fermentation


Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai -600069, India
2 Department of Biotechnology, SRM University, Kattankulathur, Chennai -603203, India
     

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This paper mainly focused to study and optimizes the parameters like carbon sources, substrate concentration, pH, Temperature for the maximum production of amylase by Aspergillus oryzae MTCC 1847. Different Carbon sources such as Sweet potato, Rice bran and Wheat Bran were to be used as the substrate for the enzyme production. The enzyme activity was observed high when the medium contained Sweet Potato as Carbon substrate with 2% concentration, pH of 6.5, Temperature of 35°C.

Keywords

Sweet Potato, Rice Bran, Wheat Bran, Aspergillus oryzae.
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  • Optimization of Amylase Production from Aspergillus oryzae MTCC 1847 by Submerged Fermentation

Abstract Views: 234  |  PDF Views: 1

Authors

S. R. Yamunarani
Department of Biotechnology, Madha Engineering College, Chennai -600069, India
M. Dinesh kumar
Department of Biotechnology, Madha Engineering College, Chennai -600069, India
B. Balachander
Department of Biotechnology, Madha Engineering College, Chennai -600069, India
K. Tamilarasan
Department of Biotechnology, Madha Engineering College, Chennai -600069, India
C. Muthukumaran
Department of Biotechnology, SRM University, Kattankulathur, Chennai -603203, India

Abstract


This paper mainly focused to study and optimizes the parameters like carbon sources, substrate concentration, pH, Temperature for the maximum production of amylase by Aspergillus oryzae MTCC 1847. Different Carbon sources such as Sweet potato, Rice bran and Wheat Bran were to be used as the substrate for the enzyme production. The enzyme activity was observed high when the medium contained Sweet Potato as Carbon substrate with 2% concentration, pH of 6.5, Temperature of 35°C.

Keywords


Sweet Potato, Rice Bran, Wheat Bran, Aspergillus oryzae.