Research Journal of Pharmaceutical Dosage Form and Technology

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Induced Mutation, Development of Multiple Analogue Resistant Strain and Protoplast Fusion for L-Methionine Fermentation by Corynebacterium glutamicum


Affiliations
1 Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, India
2 Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha
3 Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, West Bengal, India
     

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Corynebacterium glutamicum X1 , a L-glutamic acid producing wild strain which accumulated mild amount of Lmethionine (only 0.6 mg/ml) in the broth ,was subjected to mutational treatments using EMS and UV irradiations to develop a high L-methionine producing mutant. High L-methionine yielding strain Corynebacterium glutamicum X168 was selected in this study which was then subjected to protoplast fusion with a multiple L-methionine analogueresistant strain Corynebacterium glutamicum X124. A high L-methionine yielding, multiple L-methionine analogue resistant mutant Corynebacterium glutamicum X300 was derived in this study which accumulated 9.6 mg /ml Lmethionine in the fermentation broth.

Keywords

Corynebacterium glutamicum , L-Glutamic Acid , L-Methionine, Analogue Resistant.
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  • Induced Mutation, Development of Multiple Analogue Resistant Strain and Protoplast Fusion for L-Methionine Fermentation by Corynebacterium glutamicum

Abstract Views: 265  |  PDF Views: 0

Authors

Subhadeep Ganguly
Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, India
Kunja Bihari Satapathy
Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha
Ajit Kumar Banik
Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, West Bengal, India

Abstract


Corynebacterium glutamicum X1 , a L-glutamic acid producing wild strain which accumulated mild amount of Lmethionine (only 0.6 mg/ml) in the broth ,was subjected to mutational treatments using EMS and UV irradiations to develop a high L-methionine producing mutant. High L-methionine yielding strain Corynebacterium glutamicum X168 was selected in this study which was then subjected to protoplast fusion with a multiple L-methionine analogueresistant strain Corynebacterium glutamicum X124. A high L-methionine yielding, multiple L-methionine analogue resistant mutant Corynebacterium glutamicum X300 was derived in this study which accumulated 9.6 mg /ml Lmethionine in the fermentation broth.

Keywords


Corynebacterium glutamicum , L-Glutamic Acid , L-Methionine, Analogue Resistant.