Research Journal of Pharmaceutical Dosage Form and Technology

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New Spectrophotometric Method for Assay of Amphotericin B in Bulk and Its Pharmaceutical Formulations


Affiliations
1 Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda, Andhrapradesh, India
2 Krishna Teja Pharmacy College, Tirupati, India
     

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Two simple and sensitive visible spectrophotometric methods (A and B) have been developed for the determination of Amphotericin B (AMP) in bulk and pharmaceutical formulations. These methods are based on the Method A was developed based on the formation of a colored condensation reaction with p-dimethyl amino cinnamaldehyde (PDAC) under acidic conditions (λmax 490 nm), and method B based on the oxidative coupling reaction with DCQC (2,6-dichloroquinone N-chlorimide) (λmax 530nm) . Beer's law limits, precision and accuracy of these methods are checked by the UV reference method. The results obtained are reproducible and are statistically validated and so found to be suitable for the assay of in bulk and pharmaceutical formulations.
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  • New Spectrophotometric Method for Assay of Amphotericin B in Bulk and Its Pharmaceutical Formulations

Abstract Views: 284  |  PDF Views: 0

Authors

Mohan Krishna Lokireddy
Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda, Andhrapradesh, India
P. Jayachandra Reddy
Krishna Teja Pharmacy College, Tirupati, India
M. Srinivasa Murthy
Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda, Andhrapradesh, India

Abstract


Two simple and sensitive visible spectrophotometric methods (A and B) have been developed for the determination of Amphotericin B (AMP) in bulk and pharmaceutical formulations. These methods are based on the Method A was developed based on the formation of a colored condensation reaction with p-dimethyl amino cinnamaldehyde (PDAC) under acidic conditions (λmax 490 nm), and method B based on the oxidative coupling reaction with DCQC (2,6-dichloroquinone N-chlorimide) (λmax 530nm) . Beer's law limits, precision and accuracy of these methods are checked by the UV reference method. The results obtained are reproducible and are statistically validated and so found to be suitable for the assay of in bulk and pharmaceutical formulations.