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Production of Industrially Significant Enzymes from Bio-Wastes Using Aspergillus niger by Solid State Fermentation


Affiliations
1 CMJ University, Meghalaya, India
2 Noida International University, Greater Noida, India
3 Delhi University, New Delhi, India
4 Microbiology Lab., Dabur India Ltd. Ghaziabad, India
     

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In this study the production of cellulases from Aspergillus niger by solid state fermentation was performed using bio wastes viz; sugarcane bagasse and wheat bran. One factor at a time approach was used to optimize the variables such as pH, substrate ratio and temperature. A maximum of 988.2 IU/gm activity of enzyme is observed at substrates ratio of 2:1 (sugarcane bagasse: wheat bran) at 30°C and pH 5 after 5 days incubation. The high cost of production of Cellulotic enzymes has hindered the industrial application of cellulose bioconversion. Hence, this study showed an effective utilization of sugarcane bagasse for the production of cellulotic enzymes which could be effectively used in degradation of cellulose into smaller glucose units in a cost effective manner.

Keywords

Sugarcane Bagasse, Wheat Bran, Aspergillus niger, Cellulase, Cellulose.
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  • Production of Industrially Significant Enzymes from Bio-Wastes Using Aspergillus niger by Solid State Fermentation

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Authors

Tara Chand
CMJ University, Meghalaya, India
Fanish K. Pandey
Noida International University, Greater Noida, India
Shruti Dhingra
Delhi University, New Delhi, India
Manoj K. Sharma
Microbiology Lab., Dabur India Ltd. Ghaziabad, India

Abstract


In this study the production of cellulases from Aspergillus niger by solid state fermentation was performed using bio wastes viz; sugarcane bagasse and wheat bran. One factor at a time approach was used to optimize the variables such as pH, substrate ratio and temperature. A maximum of 988.2 IU/gm activity of enzyme is observed at substrates ratio of 2:1 (sugarcane bagasse: wheat bran) at 30°C and pH 5 after 5 days incubation. The high cost of production of Cellulotic enzymes has hindered the industrial application of cellulose bioconversion. Hence, this study showed an effective utilization of sugarcane bagasse for the production of cellulotic enzymes which could be effectively used in degradation of cellulose into smaller glucose units in a cost effective manner.

Keywords


Sugarcane Bagasse, Wheat Bran, Aspergillus niger, Cellulase, Cellulose.