Wound Healing Activity of Poly Herbal Formulation

Aim: To evaluate the Wound healing potential of a Poly herbal formulation.

Material and Methods: In the present work a poly herbal Unani formulation; beeswax (base), sesame oil (10%), olive oil (10%), honey (20%), psoraleac (5%), borax (10%), arnica (5%) and curcuma longa (1%) were used and the wound healing potential was determined by both excision and incision wound healing models. Albino wistar rats were used for this purpose. The wound area in excision wound model and tensile strength in incision wound model were determined. Histopathological studies were also carried out to determine the phases of wound healing.

Results: From the results obtained it was evident that the PUF has shown wound healing activity in both excision and incision wound models. In the EWM the beneficial effect of the PUF was evident from the day of first measurement as the progressive decrease in wound area was observed. On the 2^nd day of wounding, the wound area shrinkage in 10% formulation treated group was more when compared to the other test groups. The wound area was decreasing constantly and predominantly in all test treated groups as compared to the negative control group. In the IWM there was decrease in tensile strength of the animals treated with the 10% formulation and was comparable with the standard group. In the histopathological examination, the phases in the wound healing process were observed and it showed that proliferation phase was the predominant stage of action of PUF. The best re-modeling, particularly, re-epithelization were detected with the 10% formulation group On the other hand; faster keratinization characterized with minor intraepithelial cornification was seen in 15% group.