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Preparation, Characterization and Anti-Inflammatory Activity of Chitosan Stabilized Silver Nanoparticles
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The present study deals with preparation of silver nanoparticles using green approach such as polysaccharides method. (Chitosan was used as reducing and stabilizing agent). The prepared silver nanoparticles were characterized by UV Visible Spectroscopy, Fourier Transform Infra- red spectroscopy, Photon Correlation Spectroscopy, Scanning Electron Microscope and ICPOES. UV- Visible spectrum shows the SPR band at 420nm which confirms the formation of silver nanoparticles. The average particle size of chitosan stabilized silver nanoparticles was found to be 127.3nm. The amount of silver present in the solution was found to be 1.259mg/L using ICPOES. Chitosan stabilized silver nanoparticles were studied for anti-inflammatory activity. In vitro toxicity studies were carried out in 3T3/NIH fibroblast cell line. Using the natural environmental benign polymer chitosan, the silver nanoparticles were prepared and characterized.
Keywords
Chitosan, Silver Nanoparticles, SPR Band, SEM, ICPOES, Membrane Stabilizing Activity.
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