Research Journal of Pharmaceutical Dosage Form and Technology

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The Estimation of Maraviroc in Tablet dosage form by RP-HPLC


Affiliations
1 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, India
2 Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Visakhapatnam- 530003, India
3 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, India
     

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A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Maraviroc in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5m partical size, with mobile phase consisting of 0.02M Dipotasium hydrogen orthophosphate in water pH 2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 0.8 ml/min and the effluents were monitored at 210 nm. The retention time was 4.330 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y= 31018.059X + 44222.4. The limit of detection and limit of quantification was 0.2μg and 0.6μg/ml respectively. The percentage assay of Maraviroc was 99.42%. The method was validated by determining its accuracy, precision and system suitability.

The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Maraviroc in bulk drug and in its pharmaceutical dosage form.


Keywords

Maraviroc, RP-HPLC, Estimation, and Tablets.
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  • The Estimation of Maraviroc in Tablet dosage form by RP-HPLC

Abstract Views: 275  |  PDF Views: 0

Authors

L. Satyanarayana
Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, India
S. V. Naidu
Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Visakhapatnam- 530003, India
M. Narasimha Rao
Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Visakhapatnam- 530003, India
C. Ayyanna
Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Visakhapatnam- 530003, India
Alok Kumar
Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, India

Abstract


A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Maraviroc in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5m partical size, with mobile phase consisting of 0.02M Dipotasium hydrogen orthophosphate in water pH 2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 0.8 ml/min and the effluents were monitored at 210 nm. The retention time was 4.330 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y= 31018.059X + 44222.4. The limit of detection and limit of quantification was 0.2μg and 0.6μg/ml respectively. The percentage assay of Maraviroc was 99.42%. The method was validated by determining its accuracy, precision and system suitability.

The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Maraviroc in bulk drug and in its pharmaceutical dosage form.


Keywords


Maraviroc, RP-HPLC, Estimation, and Tablets.