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Isolation and Characterization of Antibiotic Production from Soil Isolates by Fermentation


Affiliations
1 KLE College of Pharmacy, Nipani, Karnataka, India
2 KLES’s College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka, India
3 KLES’s College of Pharmaceutical Sciences, Nipani, Karnataka, India
     

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In screening of new antibiotics, several actinomycetes were isolated from soil samples. Crowded plate technique was used for the isolation of actinomycetes. The morphological and cultural characterization of A-4 strain was performed. In medium formulation study for A-4 and A-4 mutant, various carbon and nitrogen sources were tested for maximum antibiotic production using zone of inhibition and packed cell volume (%) as parameters. Various fermentation conditions like pH, temperature and DO2 were also optimized for the maximal production of antibiotic from both A-4 and A-4 mutant. All medium formulation as well as bioprocess parameters for A-4 and A-4 mutant strains was compared. Some actinomycetes strains, showed promising antimicrobial scores against different strains of bacteria and fungi. From the six strains selected, one strain designated as A-4 showed maximum antimicrobial property against gram positive and gram negative strains as well as various fungi. Morphological and cultural studies showed that A-4 is belongs to actinomycete genus.. The strain A-4 and A-4 mutant was found to be having better antimicrobial activity in comparison with other soil isolates of actinomycetes.


Keywords

Actinomycete, Antibiotic, Crowded Plate Technique, Zone of Inhibition, Fermentation.
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  • Isolation and Characterization of Antibiotic Production from Soil Isolates by Fermentation

Abstract Views: 272  |  PDF Views: 0

Authors

S. Chandrashekhara
KLE College of Pharmacy, Nipani, Karnataka, India
B. K. Nanjwade
KLES’s College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka, India
P. S. Goudanavar
KLES’s College of Pharmaceutical Sciences, Nipani, Karnataka, India
F. V. Manvi
KLES’s College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka, India
M. Shamrez Ali
KLES’s College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka, India

Abstract


In screening of new antibiotics, several actinomycetes were isolated from soil samples. Crowded plate technique was used for the isolation of actinomycetes. The morphological and cultural characterization of A-4 strain was performed. In medium formulation study for A-4 and A-4 mutant, various carbon and nitrogen sources were tested for maximum antibiotic production using zone of inhibition and packed cell volume (%) as parameters. Various fermentation conditions like pH, temperature and DO2 were also optimized for the maximal production of antibiotic from both A-4 and A-4 mutant. All medium formulation as well as bioprocess parameters for A-4 and A-4 mutant strains was compared. Some actinomycetes strains, showed promising antimicrobial scores against different strains of bacteria and fungi. From the six strains selected, one strain designated as A-4 showed maximum antimicrobial property against gram positive and gram negative strains as well as various fungi. Morphological and cultural studies showed that A-4 is belongs to actinomycete genus.. The strain A-4 and A-4 mutant was found to be having better antimicrobial activity in comparison with other soil isolates of actinomycetes.


Keywords


Actinomycete, Antibiotic, Crowded Plate Technique, Zone of Inhibition, Fermentation.