Evaluation of In-Vitro Anti-Oxidant Activity of Various Extracts of Actinodaphne madraspatana Leaves

The present study was designed to investigate the in-vitro anti-oxidant activity of methanolic (MEAM), ethyl acetate (EAEAM), chloroform (CEAM) and aqueous (AEAM) extracts of leaves of Actinodaphne madraspatana (AM). MEAM, EAEAM, CEAM and AEAM extracts of leaves were also subjected to the preliminary phytochemical screening. The in-vitro models selected for the present study are DPPH (Di- Phenyl Picryl Hydrazine) free radical scavenging method, Nitric Oxide (NO) free radical scavenging method and Reducing power assay (RPA). Ascorbic acid was used as a standard in these three methods. The first two methods include the direct measurement of free radical scavenging activity whereas the remaining method includes the evaluation of reducing power. IC50 and R2 values of MEAM, EAEAM, CEAM and AEAM were also calculated in DPPH and Nitric Oxide free radical scavenging methods. The preliminary phytochemical screening of MEAM, EAEAM, CEAM and AEAM extracts of leaveshas revealed the presence of various bioactive principles such as tannins, flavonoids, saponins and steroids. From the present study we can propose that MEAM, EAEAM, CEAM and AEAM leaf extracts possess a significant anti-oxidant activity. However, further investigations are to be undertaken in order to elucidate the mechanisms of anti-oxidant activities of leaf extracts of Actinodaphne madraspatana.