Evaluation of Antibacterial, Analgesic and Antipyretic Activity of Leucas aspera Spreng

Aim of the present study is to investigate preliminary phyto-chemical screening and to evaluate plant extracts for analgesic, antipyretic and antimicrobial activities of different parts of Leucas aspera spreng by standard experimental methods which include anti-writhing method for analgesic activity, cup-plate diffusion method for antimicrobial activity and baker's yeast induced pyrexia method for antipyretic activity. Different parts of the plant like bud, ischolar_main, leaf, and stem were selected and extracted by cold maceration for 72 hrs. by using ethyl acetate and methanol as solvents. All crude extracts were used for preliminary phyto chemical screening. Ethyl acetate extracts were found be positive for alkaloids, glycosides, steroids, carbohydrates and terpenes. Methonolic extracts were found to be positive for alkaloids, glycosides, carbohydrates and terpenes and negative for steroids, flavonoids. In antimicrobial study all crude extract were found to be active against tested organisms. Ethyl acetate extracts were active against Gram-negative bacteria. Methonolic extracts were active against Grampositive bacteria .Among all the parts bud has maximum anti-bacterial activity followed by ischolar_main, leaf, stem .In analgesic activity ethyl acetate extracts of bud produce significant inhibition in acetic acid induced writhing's in mice at the dose of 100mg/kg and 200mg/kg. The same ethyl acetate extracts also showed significant antipyretic activity than methonolic extracts. These results suggest conformation of its traditional uses (antimicrobial, analgesic as well as antipyretic activities) of a plant and among different parts of plant bud has shown maximum activity.