Simultaneous Determination of Eight Phytoconstituents in Triphala churna by HPLC–DAD

Triphala is one of the most popular herbal preparations in the world used to treat variety of diseases. A simple, precise and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous estimation of gallic acid, methyl gallate, ethyl gallate, chebulagic acid, tetra-O-galloyl- β-D-glucose, ellagic acid, chebulinic acid and penta-O-galloyl-β-D-glucose in triphala churna. The chromatographic separation was achieved on Inertsil ODS-3 column (100mm×4. 6mm, 3μm). A mixture of 0.02% triethyl amine aqueous pH 3.0 with ortho-phosphoric acid (A) and acetonitrile (B) was used as mobile phase in gradient mode at a flow rate of 1. 0 ml/min and detector wavelength at 272 nm. The validation of the proposed method was carried out for linearity, accuracy, precision, limit of detection, limit of quantification and robustness. The results from intra and inter day validation showed the method was efficient and reproducible. The advantage of the developed method is simple extraction procedure involved and short run time (27 min.). Hence this method can be used for routine quality control analysis of Triphala churna.