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Preliminary Pharmacognostical and Phytochemical Studies of Leaves of Hemigraphis colorata


Affiliations
1 Sarada Vilas College of Pharmacy, Mysore- 570004, Karnataka, India
2 Sarada Vilas College of Pharmacy, Krishnamurthypuram, Mysore-570004, Karnataka, India
3 Marata Mandal College of Pharmacy, Belgaum, Karnataka, India
4 Genba Sopanrao Moze College of Pharmacy, Pune, India
     

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The aim of this research was to develop the pharmacognostical parameters and phytochemical screening alongwith histological studies and quantitative microscopy of the leaf powder of Hemigraphis colorata.

The dried leaves of Hemigraphis colorata (Family: Acanthaceae) were subjected to standardization by following pharmacognostical and phytochemical screening methods. Methanolic extract was subjected to thin layer chromatography (TLC) to find out various phytoconstituents.

Microscopic study showed the presence of Cuticle, covering trichome, single layered thick walled epidermis, palisade cells, transfusion tissue, spongy cells, vascular bundle and chlorenchyma. Phytochemical screening reported the presence of alkaloids, tannins, proteins; steroids and sterols were present in Methanolic extract. TLC showed two different Rf values (0.86 and 0.36) with different nature of their residues.


Keywords

Phytochemical screening, TLC, Hemigraphis Colorata.
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  • Preliminary Pharmacognostical and Phytochemical Studies of Leaves of Hemigraphis colorata

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Authors

J. Saravanan
Sarada Vilas College of Pharmacy, Mysore- 570004, Karnataka, India
W. R. Shariff
Sarada Vilas College of Pharmacy, Krishnamurthypuram, Mysore-570004, Karnataka, India
H. Joshi Narasimhachar
Sarada Vilas College of Pharmacy, Krishnamurthypuram, Mysore-570004, Karnataka, India
R. Varatharajan
Sarada Vilas College of Pharmacy, Krishnamurthypuram, Mysore-570004, Karnataka, India
V. G. Joshi
Marata Mandal College of Pharmacy, Belgaum, Karnataka, India
A. Karigar Asif
Genba Sopanrao Moze College of Pharmacy, Pune, India

Abstract


The aim of this research was to develop the pharmacognostical parameters and phytochemical screening alongwith histological studies and quantitative microscopy of the leaf powder of Hemigraphis colorata.

The dried leaves of Hemigraphis colorata (Family: Acanthaceae) were subjected to standardization by following pharmacognostical and phytochemical screening methods. Methanolic extract was subjected to thin layer chromatography (TLC) to find out various phytoconstituents.

Microscopic study showed the presence of Cuticle, covering trichome, single layered thick walled epidermis, palisade cells, transfusion tissue, spongy cells, vascular bundle and chlorenchyma. Phytochemical screening reported the presence of alkaloids, tannins, proteins; steroids and sterols were present in Methanolic extract. TLC showed two different Rf values (0.86 and 0.36) with different nature of their residues.


Keywords


Phytochemical screening, TLC, Hemigraphis Colorata.