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In Vitro Antibacterial and Antifungal Assay of Tectona grandis-A Screening Study


Affiliations
1 Department of Industrial Biotechnology, Dr. M. G. R. Educational and Research Institute, Dr. M. G. R .University, Maduravoyal, Chennai-600 095, Tamil Nadu, India
2 Dr. M.G.R. Educational and Research Institute, Dr. M. G. R University, Maduravoyal, Chennai-600 095, Tamil Nadu, India
3 Sri Ramachandra Medical College and Research Institute, Chennai, Tamil Nadu, India
     

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Medicinal plants are potential of antimicrobial compounds. The present study deals with the antibacterial and antifungal activity of methanolic extracts of Tectona grandis (Verabinaceae) leaves were collected from the garden of Dr. M.G.R. University during May- June 2009, Chennai, India. Air- dried and extracted by methanol. The methanolic extract of Tectona grandis leaves were examined for antibacterial and antifungal activity by in vitro using the disc diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum fungicidal Concentration (MFC). Activity against Gram-positive (Streptococcus species (MTCC 389)), Gram-negative bacteria (Klebsiella pneumonia (MTCC 432), Psedomonas aeruginosa (MTCC 1688), Proteus mirabilis (MTCC 425), Escherichia coli, (MTCC 729), Salmonella typhimurium(MTCC 98))and fungal strains (Candida albicans, Cryptococcus neoformans, Aspergillus flavus, Aspergillus niger Aspergillus fumigatus Rhizopus sp. Trichophyton rubrum,Trichophyton gypseum and Trichophyton mentagrophytes) is discussed.


Keywords

Antibacterial Activity, Antifungal Activity, Disc Diffusion Method, MIC, MBC, MFC, Tectona grandis.
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  • In Vitro Antibacterial and Antifungal Assay of Tectona grandis-A Screening Study

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Authors

K. G. Purushotham
Department of Industrial Biotechnology, Dr. M. G. R. Educational and Research Institute, Dr. M. G. R .University, Maduravoyal, Chennai-600 095, Tamil Nadu, India
P. Arun
Dr. M.G.R. Educational and Research Institute, Dr. M. G. R University, Maduravoyal, Chennai-600 095, Tamil Nadu, India
J. Johnsy Jayarani
Dr. M.G.R. Educational and Research Institute, Dr. M. G. R University, Maduravoyal, Chennai-600 095, Tamil Nadu, India
R. Vasanthakumari
Dr. M.G.R. Educational and Research Institute, Dr. M. G. R University, Maduravoyal, Chennai-600 095, Tamil Nadu, India
D. Chamundeeswari
Sri Ramachandra Medical College and Research Institute, Chennai, Tamil Nadu, India

Abstract


Medicinal plants are potential of antimicrobial compounds. The present study deals with the antibacterial and antifungal activity of methanolic extracts of Tectona grandis (Verabinaceae) leaves were collected from the garden of Dr. M.G.R. University during May- June 2009, Chennai, India. Air- dried and extracted by methanol. The methanolic extract of Tectona grandis leaves were examined for antibacterial and antifungal activity by in vitro using the disc diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum fungicidal Concentration (MFC). Activity against Gram-positive (Streptococcus species (MTCC 389)), Gram-negative bacteria (Klebsiella pneumonia (MTCC 432), Psedomonas aeruginosa (MTCC 1688), Proteus mirabilis (MTCC 425), Escherichia coli, (MTCC 729), Salmonella typhimurium(MTCC 98))and fungal strains (Candida albicans, Cryptococcus neoformans, Aspergillus flavus, Aspergillus niger Aspergillus fumigatus Rhizopus sp. Trichophyton rubrum,Trichophyton gypseum and Trichophyton mentagrophytes) is discussed.


Keywords


Antibacterial Activity, Antifungal Activity, Disc Diffusion Method, MIC, MBC, MFC, Tectona grandis.