Research Journal of Pharmacognosy and Phytochemistry

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Evaluation of Anti Cancer Activity of Kydia calycina Roxb. Leaf Extract on Different Cancer Cell Lines


Affiliations
1 Department of Pharmacology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana -506009, India
     

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To study the anticancer effects of Kydia calycina using various human cancer cell cultures, in vitro by MTT assay, ROS generation and caspase-3 activities. Human cervical carcinoma (HeLa) cells, human breast cancer (MCF-7) cells and human neuroblastoma (IMR-32) cells were maintained in a 5% CO2 incubator at 37°C. Different concentrations of leaf extract of Kydia calycina in serum free culture medium were freshly prepared and used for cytotoxic activity by MTT assay, ROS generation and apoptotic effect by caspase-3 activity. The plant extract has revealed that greater percentage inhibition in all types of cancer cells in a dose dependent manner by MTT assay. The IC50 values of K. calycina extract were found to be 41.17, 46.90 and 39.49 μg/mL against HeLa, MCF-7 and IMR-32 respectively. The apoptotic activity of Kydia calycina methanolic leaf extract was evaluated through ROS generation and caspase-3 activity. The results showed that the extract has significantly increased the ROS production and caspase-3 levels in all the cell cultures in a dose dependent manner. Further studies are warranted to elucidate the anticancer activity of K. calycina.

Keywords

Kydia calycina, Cell Viability, ROS, Caspase-3, Cytotoxicity.
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  • Suppakul P, Miltz J, Sonneveld K and Bigger SW. Antimicrobial properties of basil and its possible application in food packaging. Journal of Agricultural and Food Chemistry 2003; 51: 3197-3207.

  • Arzu BY, Fatma PK and Arzu UT. In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkeya. Asian Pacific Journal of Tropical Medicine 2012; 6: 616624.

  • Hail JN, Cortes M, Drake EN and Spallholz JE. Cancer chemoprevention: a radical perspective. Free Radical Biology and Medicine 2008; 45: 97-110.

  • Kiruthika B, Palghat and Raghunathan P. Anticancer activity of Zea mays leaf extracts on oxidative stress-induced Hep2 cells. Journal of Acupuncture and Meridian Studies 2013; 6(3): 149-158.

  • Singh S, Singh PP, Roberts LR and Sanchez W. Chemopreventive strategies in hepatocellular carcinoma. Nature Reviews Gastroenterology and Hepatology 2014; 11(1): 45-54.

  • Yu SZ. Primary prevention of hepatocellular carcinoma. Journal of Gastroenterology and Hepatology 1995; 10(6): 674-82.

  • Maram S, Khilo K, Mahmoud MZ, Mahmoud GE, Walied A, Walaa A. Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellularcarcinoma cells. Asian Pacific Journal of Tropical Biomedicine 2015; 5(5): 375381.

  • Parrotta JA. Healing plants of peninsular India. CABI publishing, New Delhi. 2001.

  • Ramarao N and Henry AN. The ethanobotany of easternghats in Andhra Pradesh, India. BSI publication, Botanical survey of India. 1996.

  • Daulatabad CD, Ankalagi KK and Desai VA. Cyclopropenoid and fatty acid composition of Kydia calycina seed oil. European Journal of Lipid science and Technology 2006; 91(6): 237-238.

  • Joshi KC, Sing P and Taneja S. A sesquiterpenoid naphthol from Kydia calycina. Planta Medica 1983; 49(10): 127.

  • Baburao B, Rama NRA, Carey MW and Krishna MG. Analgesic and anti-inflammatory activities of leaf extract of Kydia calycina Roxb. Bangladesh Journal of Pharmacology 2009; 4: 101-104.

  • Parameshwar H, Babu RB, Ravi KB, Narsimha RY and Krishna MG. Hepatoprotective effect of methanolic extract of the leaves of Kydia calycina on carbon tetrachloride induced hepatotoxicity in albino rats. African Journal of Pharmacy and Pharmacology 2011; 5(16): 1920-1924.

  • Jun SL, Won-Kyo J, Myung HJ, Taek RY and Hyung KK. Sanguinarine induces apoptosis of HT-29 human colon cancer cells via the regulation of Bax/Bcl-2 ratio and caspase-9-dependent pathway. International Journal of Toxicology 2012; 31: 70-77.

  • Carmichael J, De-Graff WG, Gazdar AF, Minna JD and Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Research 1987; 47: 936-942.

  • Allely MC, Seudero DA and Monks A. Feasibility of drug screening with panels of human tumor cell lines using micro culture tetrazolium assay. Cancer Research 1998; 8: 589-601.

  • Harikiran L, Bhikku A and Narsimha RY. In vitro cytotoxicity of gold and silver nanorods using different human cell lines. Latin American Journal of Pharmacy 2015; 34: 1277-1282.

  • Min KJ, Jung KJ and Kwon TK. Carnosic acid induces apoptosis through reactive oxygen species-mediated endoplasmic reticulum stress induction in human renal carcinoma Caki cells. Journal of Cancer Prevention 2014; 19(3): 170-178.

  • Wu T, Qiang L, Chen FH, Zhao Q, Yang Z and Zou MJ. LFG-500, a newly synthesized flavonoid, induced a reactive oxygen speciesmitochondriamediated apoptosis in hepatocarcinoma cells. Biomedicine and Preventive Nutrition 2011; 1(2): 132-138.

  • Harikiran L and Narsimhareddy Y. Cytotoxicity, oxidative stress, and inflammation in human Hep G2 liver epithelial cells following exposure to gold nanorods. Toxicology Mechanisms and Methods 2016; 26: 340-347.

  • Casciola RL, Nicholson DW, Chong T, Rowan KR, Thornberry NA and Miler DK. Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death. Journal of Experimental Medicine 1996; 183: 1957-1964.

  • Abdurrahim K, Ismail K, Murat D, Fatemeh B and Baki T. Cytotoxic, genotoxic and apoptotic effects of naringenin-oxime relative to naringenin on normal and cancer cell lines. Asian Pacific Journal of Tropical Biomedicine 2016; 6(10): 872–880.

  • Maram S, Kh K, Mahmoud MZ, Mahmoud GE, Walied A and Walaa A. Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells. Asian Pacific Journal of Tropical Biomedicine 2015; 5(5): 375381.

  • Murphy MP, Holmgren A, Larsson NG, Halliwell B and Chang CJ. Unraveling the biological roles of reactive oxygen species. Cell Metabolosm 2011; 13: 361–366.

  • Wu CC and Bratton SB. Regulation of the intrinsic apoptosis pathway by reactive oxygen species. Antioxidants and Redox Signaling 2013; 19(6): 546-558.

  • Halliwell B. Oxidative stress and cancer: have we moved forward? Biochemistry Journal 2007; 401: 1-11.

  • Ahamad MS, Siddiqui S, Jafri A, Ahmad S, Afzal M and Arshad M. Induction of apoptosis and antiproliferative activity of naringenin in human epidermoid carcinoma cell through ROS generation and cell cycle arrest. PLOS One 2014; 9(10): e110003.

  • Arun BG, Atanu B, Mohammad AA. Exploring the physicochemical profile and the binding patterns of selected novel anticancer Himalayan plant derived active compounds with macromolecular targets. Informatics in Medicine Unlocked 2016; 5: 1–14.


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  • Evaluation of Anti Cancer Activity of Kydia calycina Roxb. Leaf Extract on Different Cancer Cell Lines

Abstract Views: 670  |  PDF Views: 1

Authors

Baburao Bhukya
Department of Pharmacology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana -506009, India
Harikiran Lingabathula
Department of Pharmacology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana -506009, India
Narsimhareddy Yellu
Department of Pharmacology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana -506009, India

Abstract


To study the anticancer effects of Kydia calycina using various human cancer cell cultures, in vitro by MTT assay, ROS generation and caspase-3 activities. Human cervical carcinoma (HeLa) cells, human breast cancer (MCF-7) cells and human neuroblastoma (IMR-32) cells were maintained in a 5% CO2 incubator at 37°C. Different concentrations of leaf extract of Kydia calycina in serum free culture medium were freshly prepared and used for cytotoxic activity by MTT assay, ROS generation and apoptotic effect by caspase-3 activity. The plant extract has revealed that greater percentage inhibition in all types of cancer cells in a dose dependent manner by MTT assay. The IC50 values of K. calycina extract were found to be 41.17, 46.90 and 39.49 μg/mL against HeLa, MCF-7 and IMR-32 respectively. The apoptotic activity of Kydia calycina methanolic leaf extract was evaluated through ROS generation and caspase-3 activity. The results showed that the extract has significantly increased the ROS production and caspase-3 levels in all the cell cultures in a dose dependent manner. Further studies are warranted to elucidate the anticancer activity of K. calycina.

Keywords


Kydia calycina, Cell Viability, ROS, Caspase-3, Cytotoxicity.

References