Research Journal of Pharmacognosy and Phytochemistry

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Induction of Shoots from in vitro Cultured Roots of Oldenlandia Umbellata L. – a Dyeyielding Plant


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1 K.M. Centre for Postgraduate Studies, Pondicherry-605 008, India
     

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Shoots were regenerated from the cultured ischolar_mains of Oldenlandia umbellata L. successfully for mass propagation on Murashige and Skoog (MS) medium. The nodal shoots were cultured on 3.0 mgl-1 Benzylaminopurine (BAP) to induce nodal meristems. The shoots were multiplied well on MS liquid medium supplemented with 1.0 mgl-1 BAP + 0.5 mgl-1 Indole-3 acetic acid (IAA). Shoots were ischolar_mained on half strength MS medium supplemented with 2.5 mgl-1 Indole-3 butyric acid (IBA). The in vitro produced ischolar_mains were further used as explants to initiate ischolar_main cultures to get secondary metabolites (anthraquinone derivatives/dye). The ischolar_main pieces were cultured on half strength MS medium supplemented with IBA. The multiplied ischolar_mains turned yellow in color within 4-5 weeks. Shoots were regenerated from the ischolar_mains when the ischolar_mains cultured on MS medium augmented with 1.0 mgl-1 BAP. Again these shoots were ischolar_mained with help of IBA and the plantlets were hardened in green house.

Keywords

Anthraquinone Derivatives, Oldenlandia Umbellata L., Root Culture, Shoot Regeneration.
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  • Seydel P and Dornenburg H. Establishment of in vitro plants, cell and tissue cultures from Oldenlandia affinis for the production of cyclic peptides. Plant Cell Tissue Organ Culture. 85; 2006: 247- 255.

  • Siva R. Status of natural dyes and dye yielding plants in India. Current Science. 92; 2007: 916-925.

  • Gupta M, Mazumdar UK, Thamilselvan V, Manikandan L, Senthilkumar GP, Suresh R and Kakotti BK. Potential hepatoprotective effect and antioxidant role of methanol extract of Oldenlandia umbellata in carbon tetrachloride induced hepatotoxicity in Wistar rats. Iran J. Pharmacol. Ther. 6; 2007:5- 9.

  • Rekha S, Srinivasan V, Saradha V and Hamsaveni GR. The in vitro antibacterial activity of Hedyotis umbellata. Indian J. Pharm. Sci. 68; 2006: 236-238.

  • Siva R, Mudgal G, Rajesh D, Khan FN, Vijyakumar V and Rajasekaran C. Characterization of novel pH indicator of natural dye Oldenlandia umbellata L. Nat. Prod. Res., 23; 2009a: 1210- 1217.

  • Cardon D, du Chatenet G. Guide des teintures naturelles. Delachaux et Niestle, S.A, Paris. 1990.

  • Shekhawat MS, Kannan N, Manokari M, Revathi J. In vitro Propagation of Oldenlandia umbellata L. – A Highly Medicinal and Dye-yielding Plant of Coromandel Coast. International Journal of Recent Scientific Research. 3(9), 2012: 758-761.

  • Siva R, Mayes S, Behera SK and Rajasekaran C. Anthraquinones dye production using ischolar_main cultures of Oldenlandia umbellata L. Industrial Crops and Products. 37, 2012: 415-419.

  • Murashige T and Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15; 1962: 473-497.

  • Siva R, Rajasekaran C and Mudgal G. Induction of somatic embryogenesis and organogenesis in Oldenlandia umbellata L., a dye-yielding medicinal plant. Plant Cell Tissue and Organ Culture. 98; 2009b: 205-211.

  • Franclet A and Boulay M. Rejuvenation and clonal silviculture for Eucalyptus and forest species harvested through short rotation. Biomass Production by Fast-Growing Trees. Edited by Pereira JS and Landsberg JJ. Kluwer Academic Publishers, The Netherlands. 1989; pp. 267-274.

  • Deora NS and Shekhawat NS. Micropropagation of Capparis deciduas (Forsk.) Edgew - A tree of arid horticulture. Plant Cell Report. 15; 1995: 278-281.

  • Rathore TS, Deora NS, Shekhawat NS and Singh RP. Rapid micropropagation of a tree of arid forestry Anogeissus acuminata. Biol. Plant. 35; 1993: 381-386.

  • Shekhawat MS, Shekhawat NS, Harish, Kheta Ram, Phulwaria M and Gupta AK. High frequency plantlet regeneration from nodal segment culture of female Momordica dioica (Roxb.) Journal of Crop Science and Biotechnology. 14(2), 2011: 133-137.

  • Shekhawat MS, Shekhawat NS. Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and suspension culture. Acta Physiologiae Plantarum, 33; 2011: 1445-1450.

  • Phulwaria M, Rai MK, Harish, Gupta AK, Ram K and Shekhawat NS. An improved micropropagation of Terminalia bellirica from nodal explants of mature tree. Acta Physiologiae Plantarum, (in press). 2011. DOI 10.1007/s11738-011-0828-3.


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  • Induction of Shoots from in vitro Cultured Roots of Oldenlandia Umbellata L. – a Dyeyielding Plant

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Authors

M.S. Shekhawat
K.M. Centre for Postgraduate Studies, Pondicherry-605 008, India
N. Kannan
K.M. Centre for Postgraduate Studies, Pondicherry-605 008, India
M. Manokari
K.M. Centre for Postgraduate Studies, Pondicherry-605 008, India
J. Revathi
K.M. Centre for Postgraduate Studies, Pondicherry-605 008, India

Abstract


Shoots were regenerated from the cultured ischolar_mains of Oldenlandia umbellata L. successfully for mass propagation on Murashige and Skoog (MS) medium. The nodal shoots were cultured on 3.0 mgl-1 Benzylaminopurine (BAP) to induce nodal meristems. The shoots were multiplied well on MS liquid medium supplemented with 1.0 mgl-1 BAP + 0.5 mgl-1 Indole-3 acetic acid (IAA). Shoots were ischolar_mained on half strength MS medium supplemented with 2.5 mgl-1 Indole-3 butyric acid (IBA). The in vitro produced ischolar_mains were further used as explants to initiate ischolar_main cultures to get secondary metabolites (anthraquinone derivatives/dye). The ischolar_main pieces were cultured on half strength MS medium supplemented with IBA. The multiplied ischolar_mains turned yellow in color within 4-5 weeks. Shoots were regenerated from the ischolar_mains when the ischolar_mains cultured on MS medium augmented with 1.0 mgl-1 BAP. Again these shoots were ischolar_mained with help of IBA and the plantlets were hardened in green house.

Keywords


Anthraquinone Derivatives, Oldenlandia Umbellata L., Root Culture, Shoot Regeneration.

References