Research Journal of Pharmacognosy and Phytochemistry

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Antioxidant Profiling and Antibacterial Activity of the Vaccinium sikkimense from the Lachen Valley of the Sikkim Himalayas


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1 State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
     

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Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC50 value was calculated.

Keywords

V. sikkimense, Antibacterial Activity, Antioxidant Profiling, DPPH.
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  • Harborne J.B., Phytochemical methods: A guide to modern techniques on plant analysis, third edition. Kluwer Academic Publisher, United Kingdom,1998.

  • Hooker J.D., Clarke C. B. The Flora of British India. (Fl. Brit. India), 3(9): 451, 1882.

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  • Rawat, G. S. and Tambe, S. Sikkim Himalaya: Unique Features Of Biogeography and Ecology; Biodiversity of Sikkim, Govt of Sikkim, 2011.

  • Rohman, A., Riyanto, S., Yuniarti, N., Saputra, W. R., Utami, R. and Mulatsih, W. Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam); International Food Research Journal. 17: 97-106,2010.

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  • Antioxidant Profiling and Antibacterial Activity of the Vaccinium sikkimense from the Lachen Valley of the Sikkim Himalayas

Abstract Views: 436  |  PDF Views: 2

Authors

Smrita Pradhan
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
Sushen Pradhan
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
K. B. Subba
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
Bharat Basistha
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India

Abstract


Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC50 value was calculated.

Keywords


V. sikkimense, Antibacterial Activity, Antioxidant Profiling, DPPH.

References