In Vitro Anti-Alzheimer and Anti Oxidant Activity of the Peels of Citrus maxima Fruits

Alzheimer's disease is an age-associated, irreversible, progressive neurodegenerative disease which is characterized by severe memory loss, unusual behavior, personality changes, and a decline in cognitive function. Even today Alzheimer disease is spreading in very fast pace though large number of researchers and manufacturers are engaged to find effective medication but result is not satisfactory. Most of the drugs or medicines have high tendency of side effect. This fact made me inclined towards plants to execute the research work. Normally it is observed that side effects in plant derivative are very less. Most potential target for the treatment and slowing down of Alzheimer disease progression is cholinesterase inhibitors from plants. The anti oxidant and anti cholinesterase activity of various extracts of Citrus maxima fruit peels were studied by DPPH assay and Hydrogen Peroxide radical scavenging effect for in vitro analysis; Whereas Ellman's colorimetric methods were used for anti cholinesterase activity estimation. Ethanolic extract shown the potent anti oxidant (DPPH; IC₅₀=25 μg/ml , HPSA; IC₅₀=54 μg/ml) and anti cholinesterase activity with IC₅₀ value of 115 μg/ml. These plants were found to be potent enough to be studied further for its in vivo Anti Alzheimer disease and to perform formulations.