Research Journal of Pharmacology and Pharmacodynamics

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Hepatoprotective and Anti-Inflammatory Activities of Hydroalcoholic Extract of Bark of Erythrina Indica


Affiliations
1 Department of Pharmacognosy, Shree Swaminarayan Sanskar Pharmacy College, Zundal, Gandhinagar-382421, Gujarat, India
2 Troikaa Pharmaceuticals Ltd., Ahmedabad, Gujarat, India
3 Gujarat Technological University, Ahmedabad, Gujarat, India
4 Shree Swaminarayan Sanskar Pharmacy College, Zundal, Gandhinagar, Gujarat, India
     

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Background: Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver toxicity and inflammation.

Aim: This present work was done to investigate the Hepatoprotective and Anti-inflammatory activities of Hydroalcoholic extract of Bark of Erythrina indica .

Materials and Methods: Drugs like Silymarin, Carbon tetrachloride, Liquid paraffin, carrageenan, Aspirin were used. For Hepatoprotective activity SGOT, SGPT, ALKP, Total cholesterol, Total bilirubin, Albumin, Total protein Estimation kit and for anti-inflammatory activity carrageenan-induced paw oedema method were used.

Result and discussion: The hepatoprotective activity of the Hydroalcoholic extract was assessed in CCl4 induced hepatotoxic Rats. The extract of plant (300mg/kg and 500mg/kg, orally) was given for 6 days, daily and CCl4 (1 ml/kg, 1:1 with liquid paraffin, s.c.) was given at 6th day after dosing of plant extract. Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, ALKP, Bilirubin, Cholesterol, Total Protein and Albumin were tested in both CCl4 treated and untreated groups. CCl4 has enhanced the SGOT, SGPT, ALKP and Bilirubin in serum, while Total protein and Albumin were decreased. The study was also supported by histopathology of liver sections. Treatment of Hydroalcoholic extract of Bark of Erythrina indica has brought back the altered levels of biochemical markers to near about normal levels in the dose dependent manner. Antiinflammatory activity was done in rats at a dose 300 and 500 mg/kg in carrageenan-induced paw oedema method. Hydroalcoholic extract of Erythrina indica bark significantly reduced the migration of neutrophils and monocytes. Results of in vivo activity lead to the conclusion that the hydroalcoholic extract of Erythrina indica bark showed predominantly significant activity in dose dependent manner, which is comparable to the standard drug Aspirin. The present study reveals that the Hydroalcoholic extract of Bark of Erythrina indica could afford a significant Hepatoprotective and Anti-inflammatory activity.


Keywords

Erythrina indica, CCl4, SGOT, SGPT, ALKP, Aspirin, Carrageenan.
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  • Hepatoprotective and Anti-Inflammatory Activities of Hydroalcoholic Extract of Bark of Erythrina Indica

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Authors

Vrunda V. Shah
Department of Pharmacognosy, Shree Swaminarayan Sanskar Pharmacy College, Zundal, Gandhinagar-382421, Gujarat, India
Vipul K. Shah
Troikaa Pharmaceuticals Ltd., Ahmedabad, Gujarat, India
N. R. Sheth
Gujarat Technological University, Ahmedabad, Gujarat, India
M. M. Patel
Shree Swaminarayan Sanskar Pharmacy College, Zundal, Gandhinagar, Gujarat, India

Abstract


Background: Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver toxicity and inflammation.

Aim: This present work was done to investigate the Hepatoprotective and Anti-inflammatory activities of Hydroalcoholic extract of Bark of Erythrina indica .

Materials and Methods: Drugs like Silymarin, Carbon tetrachloride, Liquid paraffin, carrageenan, Aspirin were used. For Hepatoprotective activity SGOT, SGPT, ALKP, Total cholesterol, Total bilirubin, Albumin, Total protein Estimation kit and for anti-inflammatory activity carrageenan-induced paw oedema method were used.

Result and discussion: The hepatoprotective activity of the Hydroalcoholic extract was assessed in CCl4 induced hepatotoxic Rats. The extract of plant (300mg/kg and 500mg/kg, orally) was given for 6 days, daily and CCl4 (1 ml/kg, 1:1 with liquid paraffin, s.c.) was given at 6th day after dosing of plant extract. Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, ALKP, Bilirubin, Cholesterol, Total Protein and Albumin were tested in both CCl4 treated and untreated groups. CCl4 has enhanced the SGOT, SGPT, ALKP and Bilirubin in serum, while Total protein and Albumin were decreased. The study was also supported by histopathology of liver sections. Treatment of Hydroalcoholic extract of Bark of Erythrina indica has brought back the altered levels of biochemical markers to near about normal levels in the dose dependent manner. Antiinflammatory activity was done in rats at a dose 300 and 500 mg/kg in carrageenan-induced paw oedema method. Hydroalcoholic extract of Erythrina indica bark significantly reduced the migration of neutrophils and monocytes. Results of in vivo activity lead to the conclusion that the hydroalcoholic extract of Erythrina indica bark showed predominantly significant activity in dose dependent manner, which is comparable to the standard drug Aspirin. The present study reveals that the Hydroalcoholic extract of Bark of Erythrina indica could afford a significant Hepatoprotective and Anti-inflammatory activity.


Keywords


Erythrina indica, CCl4, SGOT, SGPT, ALKP, Aspirin, Carrageenan.

References