Research Journal of Pharmacology and Pharmacodynamics

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In-vitro Antioxidant Activity of Premna integrifolia Linn. Roots


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1 Department of Pharmacognosy, Smriti College of Pharmaceutical Education, 4/1, Pipliya Kumar Kakkad, Mayakhedi Road, Nipania, Indore 452010, Madhya Pradesh, India
     

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Free radicals are fairly unstable and highly reactive substances, causing oxidation and sometimes irreversible damage to cells. This has led to an increase in intake of antioxidants. Medicinal plants are being studied extensively for their antioxidant properties. The present study was aimed to study the in-vitro antioxidant activity of aqueous and methanolic extracts of Premna integrifolia Linn ischolar_mains (Verbenaceae). Both the extracts were subjected to various in-vitro antioxidant activity screening models such as DPPH, DMSO, ABTS, Nitric oxide and Iron chelation assay. Ascorbic acid was used as the standard. In all the models studied, the aqueous extract showed IC50 values of 111.009, 101.369, 99.976, 109.827, 105.239 μg/ml and the methanolic extract showed IC50 values of 98.252, 96.559, 88.163, 100.631, 95.005μg/ml for DPPH, DMSO, ABTS, Nitric oxide and Iron chelation assay, respectively.

Keywords

Free Radicals, Antioxidants, Premna integrifolia
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  • In-vitro Antioxidant Activity of Premna integrifolia Linn. Roots

Abstract Views: 966  |  PDF Views: 3

Authors

Sanjay Jain
Department of Pharmacognosy, Smriti College of Pharmaceutical Education, 4/1, Pipliya Kumar Kakkad, Mayakhedi Road, Nipania, Indore 452010, Madhya Pradesh, India
Mamta Singh
Department of Pharmacognosy, Smriti College of Pharmaceutical Education, 4/1, Pipliya Kumar Kakkad, Mayakhedi Road, Nipania, Indore 452010, Madhya Pradesh, India
Rakesh Barik
Department of Pharmacognosy, Smriti College of Pharmaceutical Education, 4/1, Pipliya Kumar Kakkad, Mayakhedi Road, Nipania, Indore 452010, Madhya Pradesh, India
Neelesh Malviya
Department of Pharmacognosy, Smriti College of Pharmaceutical Education, 4/1, Pipliya Kumar Kakkad, Mayakhedi Road, Nipania, Indore 452010, Madhya Pradesh, India

Abstract


Free radicals are fairly unstable and highly reactive substances, causing oxidation and sometimes irreversible damage to cells. This has led to an increase in intake of antioxidants. Medicinal plants are being studied extensively for their antioxidant properties. The present study was aimed to study the in-vitro antioxidant activity of aqueous and methanolic extracts of Premna integrifolia Linn ischolar_mains (Verbenaceae). Both the extracts were subjected to various in-vitro antioxidant activity screening models such as DPPH, DMSO, ABTS, Nitric oxide and Iron chelation assay. Ascorbic acid was used as the standard. In all the models studied, the aqueous extract showed IC50 values of 111.009, 101.369, 99.976, 109.827, 105.239 μg/ml and the methanolic extract showed IC50 values of 98.252, 96.559, 88.163, 100.631, 95.005μg/ml for DPPH, DMSO, ABTS, Nitric oxide and Iron chelation assay, respectively.

Keywords


Free Radicals, Antioxidants, Premna integrifolia

References