Research Journal of Pharmacology and Pharmacodynamics

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Anticancer Screening of Ficus racemosa by XTT Bioassay


Affiliations
1 JJT University, Vidyanagari, Jhunjhunu-Churu Road, Dist.: Jhunjhunu, Rajasthan - 333001, India
2 Kalol Institute of Pharmacy, Behind Old Janpath Hotel, National Highway, Kalol, Gujarat, India
     

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Ficus racemosa is an extreme sized avenue tree found throughout India. It is admired in indigenous classification of medicine like Ayurveda, Siddha, Unani in addition to Homoeopathy. Objective of the present investigation was to find out the anticancer activity of Ficus racemosa plant extracts using standard in-vitro XTT bioassay against various cancer cell lines (HL-60, HepG2, NCI-H23 & HEK-293T). With use of XTT dye, % cell viability and % cell inhibition of the hit compounds was evaluated within respective wavelengths prior with standard compounds. Data obtained from XTT bioassay screening revealed that methanolic extract of Ficus racemosa shown cytotoxic activity against HL-60 and HepG2 cell line with profound IC50 values and shown negligible toxicity against normal cell line (HEK-293T).

Keywords

Ficus racemosa Linn., XTT Bioassay, Anticancer Activity, F. glomerata.
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  • Anticancer Screening of Ficus racemosa by XTT Bioassay

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Authors

Prakash S. Sukhramani
JJT University, Vidyanagari, Jhunjhunu-Churu Road, Dist.: Jhunjhunu, Rajasthan - 333001, India
Piyush M. Patel
Kalol Institute of Pharmacy, Behind Old Janpath Hotel, National Highway, Kalol, Gujarat, India

Abstract


Ficus racemosa is an extreme sized avenue tree found throughout India. It is admired in indigenous classification of medicine like Ayurveda, Siddha, Unani in addition to Homoeopathy. Objective of the present investigation was to find out the anticancer activity of Ficus racemosa plant extracts using standard in-vitro XTT bioassay against various cancer cell lines (HL-60, HepG2, NCI-H23 & HEK-293T). With use of XTT dye, % cell viability and % cell inhibition of the hit compounds was evaluated within respective wavelengths prior with standard compounds. Data obtained from XTT bioassay screening revealed that methanolic extract of Ficus racemosa shown cytotoxic activity against HL-60 and HepG2 cell line with profound IC50 values and shown negligible toxicity against normal cell line (HEK-293T).

Keywords


Ficus racemosa Linn., XTT Bioassay, Anticancer Activity, F. glomerata.

References