Research Journal of Pharmacology and Pharmacodynamics

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Study of Anti-Inflammatory Potential of Ethanolic Fraction of Seeds of Ocimum basilicum Linn


Affiliations
1 Rajendra Institute of Technology and Sciences, 4th mile stone, Hisar Road, Sirsa - 125055, India
2 Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana), India
     

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In the present study the ethanolic fraction of seeds of the plant Ocimum basilicum Linn. was taken and its anti-inflammatory potential was evaluated using carrageenan and formalin induced rat paw edema. The effect on chronic phase of inflammation was also studied using cotton pellet granuloma technique. The increase in paw thickness was measured using digital vernier caliper after 1,2,3 and 4 h of injection. Ethanolic fraction of the seeds of the plant at dose of 400 mg/Kg significantly inhibited acute phase of inflammation. The decrease in the weight of granuloma was also found by ethanolic fraction of the seeds at same dose. The study reveals that the ethanolic fraction of seeds of Ocimum basilicum Linn. possesses significant anti-inflammatory activity.

Keywords

Anti-Inflammatory, Carrageenan, Formalin, Granuloma.
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  • Dhar, AK. Sweet Basil. Ocimum basilicum- a review. Journal of Medicinal and Aromatic Plant Sciences. 2002; 24: 738-755.

  • Jain, ML and Jain, SR. Therapeutic utility of Ocimum basilicum Linn. Planta Med. 1988; 54: 66-70.

  • Suppakul, P, Miltz, J, Sonneveld, K and Bigger, S.W: Anti microbial properties of Basil and its possible application in food packaging. J. Agricultural and Food chem. 2003; 51: 3197-3207.

  • Dasgupta, T, Rao, AR and Yadava, PK. Chemomodulatory efficacy of basil leaf on drug metabolizing and antioxidant enzymes and on the carcinogen-induced skin and forestomach papillomagenesis. Phytomedicine. 2004, 11: 139-151.

  • Singh, S and Majumdar, D K. Evaluation of gastric anti ulcer activity of fixed oil of Ocimum basilicum L. and its possible mechanism of action. Indian J. Experimental Biology. 1999; 36: 253-257.

  • Yamasaki, K, Nakano, M, Kawatha, T, Mori, H, Otake, T and Ueba, N. Anti HIV activity of herbs in Labiatae. Biological and Pharmacological Bulletin. 1998; 21: 829-833.

  • Aziba, PI, Bass, D and Elegbe, Y. Pharmacological investigation of O. gratissimum in rodents. Phytotherapy Research. 1999; 13: 427-429.

  • Zeggwagh, NA, Sulpice, T and Eddouks, M. Anti-hyperglycemic and Hypolipidemic effects of Ocimum basilicum aqueous extract in diabetic rats. American Journal of Pharmacology and Toxicology. 2007, 2 (3): 123-129.

  • Winter, CA, Risley, EA and Nuss, GW. Carrageenan -induced oedema in hind paw of rat as an assay for anti-inflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine. 1962; 111: 544-547.

  • Bonta, LL and Noordhoek, J. Anti-inflammatory mechanism of Inflamed tissue factor. Agents and Actions. 1973; 5: 519-527.

  • Lee, IO and Crosby, G. Halothane effect on formalin induced paw edema and flinching in rats. J Korean Med Sci. 1999; 14 (1): 34-38.

  • Bush, IE and Alexander, RW. An improved method for the assay of anti-inflammatory substances in rats. Acta Endocrinol. 1960; 35: 268-276.


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  • Study of Anti-Inflammatory Potential of Ethanolic Fraction of Seeds of Ocimum basilicum Linn

Abstract Views: 225  |  PDF Views: 4

Authors

Pankaj Rakha
Rajendra Institute of Technology and Sciences, 4th mile stone, Hisar Road, Sirsa - 125055, India
Manju Nagpal
Rajendra Institute of Technology and Sciences, 4th mile stone, Hisar Road, Sirsa - 125055, India
Sunil Sharma
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana), India
Milind Parle
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana), India

Abstract


In the present study the ethanolic fraction of seeds of the plant Ocimum basilicum Linn. was taken and its anti-inflammatory potential was evaluated using carrageenan and formalin induced rat paw edema. The effect on chronic phase of inflammation was also studied using cotton pellet granuloma technique. The increase in paw thickness was measured using digital vernier caliper after 1,2,3 and 4 h of injection. Ethanolic fraction of the seeds of the plant at dose of 400 mg/Kg significantly inhibited acute phase of inflammation. The decrease in the weight of granuloma was also found by ethanolic fraction of the seeds at same dose. The study reveals that the ethanolic fraction of seeds of Ocimum basilicum Linn. possesses significant anti-inflammatory activity.

Keywords


Anti-Inflammatory, Carrageenan, Formalin, Granuloma.

References