Research Journal of Pharmacology and Pharmacodynamics

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Analgesic Activity of Azima tetracantha in Experimental Animals


Affiliations
1 Post Graduate Department of Biotechnology, Jamal Mohamed College, Tiruchirappalli-620 020, Tamil Nadu, India
2 Department of Biochemistry, M.I.E.T. Arts and Science College, Tiruchirappalli-620 007, Tamil Nadu, India
     

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In the present study, the ehanolic leaf extract of Azima tetracantha Lam. (A. tetracantha) was investigated for analgesic activity in mice using hot plate method. The leaves of A. tetracantha were collected from Ponnamaravathi and cut into small pieces and shade dried. The dried powdered leaves (100 gm) were extracted in a Soxhlet apparatus by using 95% ethanol. Mice weighing 15-25 gm were taken for the experiment, divided into four groups of six animals each. Group 1 received the (normal saline 2 ml/kg) group 2 and group 3 received ethanolic leaf extract of A. tetracantha 100 and 200 mg/kg respectively and the group 4 served as standard (Pentazocine 5 mg/kg) by oral route. Each mouse was picked on the hot plate, which was maintained at constant temperature 55°C±0.05. The basal reaction time, the time taken for the jump response (or) paw licking which ever appears to be first was observed. The reaction time of animals in all groups was noted at 0, 30, 60, 120 and 180 seconds after the administration of the drug. All data were analyzed with student 't' test. A maximum analgesic activity was found at 120 sec, after drug administration, which was compared to the standard drug used as Pentazocine (5 mg/kg).

Keywords

Analgesic Activity, Mice, Azima tetracantha, Pentazocine.
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  • Ahmadiani A, Fereidoni M, Semnanian S, Kamalinejad M and Saremi S. Antinociceptive and anti-inflammatory effects of Sambucus ebulus rhizome extract in rats. Ethnopharmacol. 1998; 61: 229-235.

  • Almedia RN, Navarro DS and Barbosa-Filho JM. Plants with central analgesic activity. Phytomedicine. 2001; 8: 310-322.

  • Eddy NB and Lembach D. Synthesis, anti-inflammatory and analgesic activity of Pyridol [2, 1, 6] quinazoline derivatives. J Pharma Exp Ther. 1953; 707: 385-393.

  • Rang HP, Dale MM, Ritter JM and Moore PK. Pharmacology 5th ed. New Delhi: Churchill Livingstone Publ. 2005; P: 562.

  • Ballou LR, Botting RM, Goorha S, Zhang J and Vane JR. Nociception in cyclooxygenase isozyme-deficient mice. Proc. Natl. Acad. Sci. U.S.A. 2000; 97: 10272-10276.

  • Berkenkopf JW and Weichman BM. Production of Prostacyclin in mice following intraperitoeal injection of aceticacid phenylbenzoquinone and zymosan: it role in the writhing response. Prostaglandins. 1988; 36: 693-709.

  • Ramaswamy S, Pillai NP, Gopalkrishnan V, Parmar NS and Ghosh MN. Analgesic effect of O (Beta hydroxyethyl) rutoside in mice. Indian J. Exp. Biol. 1985; 23: 219-220.

  • Hajare SW, Suresh Chandra SK, Tandan J, Sarma J and Lal AG. Analgesic and antipyretic activities of Dalbergia sissoo leaves. Indian Journal of Pharmacology. 2000; 32: 357-360.

  • Surender S, Manish T and Dipak KM. Biological activities of Ocimum sanctum L. fixed oil. Indian J Exp Biol. 2007; 45: 403-412.

  • Sutar NG, Bonde CG, Patil VV, Narkhede SB, Patil AP and Kakade RT. Analgesic activity of seeds of Moringa oleifera Lam. Int. J Green Pharm. 2008; 2: 108-110.

  • Chakraborthy GS. Analgesic activity of various extracts of Punica granatum (Linn) flowers. Int J Green Pharm. 2008; 2: 145-146.

  • Jalapure SS, Habbu PV, Patil MB, Kulkani RV, Simpi CC and Patil CC. Analgesic and antipyretic activity of Pergularia extensa in rats. Indian J of Pharmaceuticol Sciences. 2002; 64(5): 493-495.

  • Ashok P, Prasanna GS and Mathuram V. Analgesic and antiinflammatory activity of the chloroform extract of Trichilia connaroides (W. & A.) Bentilizen. Indian J Pharm Sci. 2006; 68: 231-233.

  • Mate GS, Naikwade NS, Magdum CS, Chowki AA and Patil SB. Evaluation of antinociceptive acitivity of Cissus quadrangularis on albino mice. Int J Green Pharm. 2008; 2: 118-121.

  • Mule SN, Patil SB, Naikwade NS and Magdum CS. Evaluation of antinociceptive and anti-inflammatory activity of stems of Gynandropsis pentaphylla Linn. Int J Green Pharm. 2008; 2: 87-90.

  • Chandrashekar KS, Satyanarayana D and Joshi AB. Analgesic activitiy of Leucas lavandulaefolia. Indian drugs. 2005; 42(2): 78-80.

  • Gracioso JS, Paulo MQ, Hiruma-Lima CA and Souza Brito AR. Antinociceptive effect in mice of a hydroalcoholic extract of Neurolaena lobata (L.) R.Br. and its organic fractions. Journal of Pharmacy and pharmacology. 1998; 50(12): 1425-1429.

  • Vohera SB, Kumar I and Khan MS. Effect of alkaloids of Solanum melongena on the central nervous system. J Ethnopharmacol. 1984; 11: 331-336.

  • Telang RS, Chatterjee S and Varshneya C. Studies on analgesic and anti-inflammatory activities of Vitex negundo Linn. Indian J Pharmacol. 1999; 31: 363-366.

  • Arrigoni-Blank MF, Dmitrieva EG and Franzotti EM. Anti inflammatory and analgesic activity of Peperomia pellucida HBK (Piperaceae). J Ethnopharmacol. 2004; 91: 215-218.

  • Aziba P, Adedeji A and Ekor M. Analgesic activity of Peperomia pellucida aerial parts in mice. Fitoterapia. 2001; 72: 57-58.

  • Njunge K, Muriuki G, Mwangi JW and Kuria KA. Analgesic and antipyretic effects of Myrica salicifolia (Myricaceae). Phytother Res Mar. 2002; 16(suppl 1): S73-74.

  • Jain PS, Mallipedi S and Belsare DP. Analgesic activity of stem bark of Kigelia pinnata Linn. Indian Drugs. 2007; 44(1): 63-65.

  • Yongna Z, Wantana R, Pisit B, Zhongkun L and Rongping Z. Analgesic and antipyretic activities of aqueous extract of Urtica macrorrhiza in experimental animals. Fitoterapia. 2005; 76: 91-95.


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  • Analgesic Activity of Azima tetracantha in Experimental Animals

Abstract Views: 297  |  PDF Views: 2

Authors

T. Nargis Begum
Post Graduate Department of Biotechnology, Jamal Mohamed College, Tiruchirappalli-620 020, Tamil Nadu, India
A. Vijaya Anand
Department of Biochemistry, M.I.E.T. Arts and Science College, Tiruchirappalli-620 007, Tamil Nadu, India

Abstract


In the present study, the ehanolic leaf extract of Azima tetracantha Lam. (A. tetracantha) was investigated for analgesic activity in mice using hot plate method. The leaves of A. tetracantha were collected from Ponnamaravathi and cut into small pieces and shade dried. The dried powdered leaves (100 gm) were extracted in a Soxhlet apparatus by using 95% ethanol. Mice weighing 15-25 gm were taken for the experiment, divided into four groups of six animals each. Group 1 received the (normal saline 2 ml/kg) group 2 and group 3 received ethanolic leaf extract of A. tetracantha 100 and 200 mg/kg respectively and the group 4 served as standard (Pentazocine 5 mg/kg) by oral route. Each mouse was picked on the hot plate, which was maintained at constant temperature 55°C±0.05. The basal reaction time, the time taken for the jump response (or) paw licking which ever appears to be first was observed. The reaction time of animals in all groups was noted at 0, 30, 60, 120 and 180 seconds after the administration of the drug. All data were analyzed with student 't' test. A maximum analgesic activity was found at 120 sec, after drug administration, which was compared to the standard drug used as Pentazocine (5 mg/kg).

Keywords


Analgesic Activity, Mice, Azima tetracantha, Pentazocine.

References