Research Journal of Pharmacology and Pharmacodynamics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Phytochemical Evaluation of Extracts of Stem of Eclipta alba (Bhringaraja)


Affiliations
1 Department of Chemistry, College of Pharmacy, IFTM, Moradabad, (U.P.), India
2 School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur, (Rajasthan), 302025, India
     

   Subscribe/Renew Journal


This paper deals with the detailed phytochemical evaluation of the stem of Eclipta alba (Bhringaraja) family: (Compositae). The preliminary phytochemical screening shows that different extracts of plant have different constituents like carbohydrate, alkaloids, phenolics, steroids, glycosides, fat and oils. TLC of the hexane extract after column extraction was shown Rf value 0.78 in Petroleum ether:Hexane:Chloroform:Methanol, used as mobile phase in 2:4:2:1 ratio and TLC of pet. Ether extract also shown Rf value is 0.72 in Petroleum ether:Hexane:Chloroform, used as mobile phase in 4:3:1 ratio after column extraction. This is evaluated by the instrumental analysis (UV, IR, and NMR) of hexane and pet. Ether extract. They shows consequently that pet. Ether extract contains alkane (2940 Cm-1, δ 0.827), alkene (1630 Cm-1, δ 1.253-1.351), monomer of aliphatic acid (1760 Cm-1 δ 2.169), aromatic amine (1570 Cm-1, δ 2.593) etc. and hexane extract contains alkane (2940 cm-1, δ 0.801-0.873) alkene (1625 Cm-1, δ 0.988-1.075) aldehyde (1740 Cm-1, δ 2.154 – 2.288) and aromatic alcohol (1110Cm-1, δ 5.324). These data suggests that the extracts (pet. ether and hexane) may have the glycoside, aliphatic acid, steroids, fats and oils. They are pharmacologically important.

Keywords

Eclipta alba, Flavanoids, Glycosides, Thin Layer Chromatography.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Kritikar KR and Basu BD. Chronica Botanica Indian Medicinal plants. New Delhi. 1975

  • Chopra RN et al. Glossary of medicinal plants. CSIR publication, New Delhi. 1966: pp.104.

  • Evans WC. Trees and Evans pharmacognosy. W.B. Saunders , London. 2002; 15th ed: pp. 4-8.

  • Sharma PC et al. Database of meditional plants used in ayurveda. Vol II. Central council for research in ayurveda and siddha, New Delhi. 2001; 15th ed: pp. 590.

  • Wagner H et al. Coumestans as the Main Active Principles of the Liver Drugs Eclipta alba and Wedelia calendulacea. Plant Med. 1986; 5: 370-374.

  • Yahara S and Ning D. Taraxastane glycosides from Eclipta alba. Phyto Chem.1997; 44 (1): 131-135.

  • Upahyay RK et al. Eclalbatin, a triterpene saponin from Eclipta alba. J Asian Nat Prod Res. 2001; 3(3): 213-217.

  • Leal LKAM et al. Antinociceptive, Anti-inflammatory and bronchodilator activities of Brazilian medicinal plant containing coumarin: a comparative study. J Ethnopharmacol. 2000; 70: 151-159.

  • Kokate CK. Practical pharmacognosy. Vallabh Prakashan, Delhi. 1986b; 1th ed: pp. 111.

  • Harborne JB. Methods of Extraction and isolation. In: Phytochemical methods. Chapman and hall, London. 1998; 3rd ed: pp. 60-66.

  • Agarwal OP. Advanced practical organic chemistry. Goel publishing house, Meerut. 2000; pp. 43, 59.

  • Kothavade RJ et al. Protective effect on an Indigenous drug Livomyn on Ketoconazole Induced hepatotoxicity. Indian J Pharm Sci. 1996; 58(4): 142-146.

  • Singh B et al. In vivo hepatoprotective activity of active fraction from ethanolic extract of Eclipta alba leaves. Indian J Physiol Pharmacol. 2001; 45(4): 435-441.

  • Zhang X et al. Screening of antiviral agents from medicinal herbs by means of Hepadnaviruses models. Zhonggno Zhong Yao Za Zi. 1996; 21(8): 480-1, 510.

  • Kumar S et al. Antibacterial activity observed in the seeds of some Coprophilous plants. Indian J Pharm. 1997; 35(3): 179-184.

  • Thakur VD and Mengi SA. Neuropharmacological profile of Eclipta alba (Linn.) Hassk. J Ethnopharmacol. 2005; 102(1): 23-31.

  • Sawant M et al. Analgesic studies on total alkaloids and alcohol extracts of Eclipta alba (Linn.) Hassk. Phytother Res. 2004; 18(2): 111-113.

  • Ananthi J et al. Antihyperglycemic activity of Eclipta alba leaf on alloxan-induced diabetic rats. Yale J Biol Med. 2003; 76(3): 97-102.

  • Khandeparkar UK and Kulkarni RD. Antifatigue effect of indigenous drug 'Geriforte' in rats. Indian Drugs. 1981; 18(10): 346-349.

  • George M et al. A search for Antibiotic substance in some Indian medicinal plants. J Sci Indian Res. 1947; B: 42-46.

  • Kishore N and Dubey NK. Fungitoxicity of some higher plant against Trichophyton rubrum and Epidermophyton floccossum. Indian J Pharm Sci. 1988; 50 (60): 323-325.

  • Venaktsam S and Ravi R. Antifungal Activity of Eclipta alba. Indian J Pharm Sci. 2004; Jan- feb: 97-98.

  • Roy RK et al. Development and evaluation of polyherbal formulation for hair growth-promoting activity. J Cosmet Dermatol. 2007; 6(2): 108-112.

  • Rangineni V et al. Diuretic, hypotensive, and hypocholesterolemic effects of Eclipta alba in mild hypertensive subjects: a pilot study. J Med Food. 2007; 10(1): 143-148.

  • Christybapita D et al. Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus. Fish Shellfish Immunol. 2007; 23(4): 840-52.

  • Jayathirtha MG and Mishra SH. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine. 2007; 11(4): 361-365.


Abstract Views: 200

PDF Views: 0




  • Phytochemical Evaluation of Extracts of Stem of Eclipta alba (Bhringaraja)

Abstract Views: 200  |  PDF Views: 0

Authors

A. Agarwal
Department of Chemistry, College of Pharmacy, IFTM, Moradabad, (U.P.), India
V. Gupta
School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur, (Rajasthan), 302025, India
Amita Gupta
School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur, (Rajasthan), 302025, India
S. Jayalakshmi
Department of Chemistry, College of Pharmacy, IFTM, Moradabad, (U.P.), India

Abstract


This paper deals with the detailed phytochemical evaluation of the stem of Eclipta alba (Bhringaraja) family: (Compositae). The preliminary phytochemical screening shows that different extracts of plant have different constituents like carbohydrate, alkaloids, phenolics, steroids, glycosides, fat and oils. TLC of the hexane extract after column extraction was shown Rf value 0.78 in Petroleum ether:Hexane:Chloroform:Methanol, used as mobile phase in 2:4:2:1 ratio and TLC of pet. Ether extract also shown Rf value is 0.72 in Petroleum ether:Hexane:Chloroform, used as mobile phase in 4:3:1 ratio after column extraction. This is evaluated by the instrumental analysis (UV, IR, and NMR) of hexane and pet. Ether extract. They shows consequently that pet. Ether extract contains alkane (2940 Cm-1, δ 0.827), alkene (1630 Cm-1, δ 1.253-1.351), monomer of aliphatic acid (1760 Cm-1 δ 2.169), aromatic amine (1570 Cm-1, δ 2.593) etc. and hexane extract contains alkane (2940 cm-1, δ 0.801-0.873) alkene (1625 Cm-1, δ 0.988-1.075) aldehyde (1740 Cm-1, δ 2.154 – 2.288) and aromatic alcohol (1110Cm-1, δ 5.324). These data suggests that the extracts (pet. ether and hexane) may have the glycoside, aliphatic acid, steroids, fats and oils. They are pharmacologically important.

Keywords


Eclipta alba, Flavanoids, Glycosides, Thin Layer Chromatography.

References