Research Journal of Pharmacology and Pharmacodynamics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Evaluation of Genotoxic Effect of Cashew Nut Kernel Oil


Affiliations
1 East Point College of Pharmacy, Bidarahalli, Virgonagar Post, Bangalore - 560 049, India
2 East Point College of Pharmacy, # 147, Bidarahalli, Virgonagar Post, Bangalore - 560 049, India
3 NGSM Institute of Pharmaceutical Sciences, Palneer, Mangalore - 575 005, India
     

   Subscribe/Renew Journal


The present study has been carried out to investigate the genotoxic effect of Cashew nut kernel oil by Drosophila melanogaster (fruit fly) and on Allium cepa ischolar_main (meristamatric tip) methods. Different concentration of Cashew nut kernel oil in ethanol were selected and subjected for sterility test and dominant lethal test by using Drosophilla. For Allium cepa method, each concentration of test oil was applied for different times 12h, 24h and 48h. The obtained result indicates that the cashew nut kernel oil had the ability to cause genotoxic effect at 0.03% v/v concentration in ethanol and also cause different mitotic changes. These various changes appeared in depending on the duration of treatment. The results obtained from both sterility test and dominant lethal test found that Cashew nut kernel oil increases the lethality in flies while the concentration of oil increases.

Keywords

Alliumcepa, Cashew Nut Kernel Oil, Drosophila, Chromosome Abnormalities.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ashby J, De Serres FJ, Draper M, Ishidate M, Margolin BH, Matter BE, Shelby MD. Evaluation of short-term tests for carcinogens. Report of the International experiments in genetics with drosophila.1985; 13(2): 85-86.

  • Bartsch H, Kurokit, Roberfroid M and Malaveille C. Metabolic activation systems in vitro for carcinogen-mutagen screening tests. Vol. 7 Plenum Press, New York. 1971. 95-161.

  • Grafe VJ. Small numbers in mutagenicity tests. Arch. Toxicol. 1977; 38: 27-34.

  • Killeen AA, Rubin E and Strayer DS. Developmental and Genetic Diseases. In Rubin's Pathology: Clinico pathologic foundations of medicine. Edited by Rubin R and Strayer DS. Lippincott Williams & Wilkins, Philadelphia. 2008; 5th pp. 177-228.

  • Mohan H. Essential pathology for dental students. Jaypee Brothers Medical Publishers, New Delhi. 2002.

  • Vidakovic-Cifrek Z , Paylica M, Regula I and Papes D. Cytogenetic Damage in Shallot (Allium cepa) Root Meristems Induced by Oil Industry "High-Density Brines. Arch. Environ. Contam. Toxicol. 2002; 43: 284–291.

  • Pontecorvo, G, Roper JA, Hemmons lM, Macdonald KD and Ufton W J. The genetics of Aspergillus nidulans. Advances in Genetics. 1953; 5: 141-238.

  • Kihlman BA and Andersson HC. Sister chromatid exchanges in plants. John Wiley and Sons, New York. 1982.

  • Monroe W and Strickberger. Experiments in Genetics with Drosophila. John Wiley and Sons, New York. 1962.

  • UAB Center for Community OutReach Development. Drosophila Genetics. University of Alabama; Birmingham. 1998.

  • Latt SA, Allen J. Bloom SE, Carrano A, Falke E, Kram D, Schneiderm E, Schreck R, Rice R and Whitfield B. Sister-chromatid exchanges: a report of the Gene-Tox Programme. Mutant. 1981; 87: 17-62.

  • Demerec M and Kaufman BP, Drosophila guide introduction to the genetics and cytology of Drosophila Melanogaster. Institute of Washington, 1973.

  • Abbrahamson S and Lewis EB. The detection of mutations in Drosophila melanogaster principles and methods for their detection. Plenum Press, New York, London, 1971.

  • Amin AW. Cytotoxicity testing of sewage water treatment using Allium cepa chromosome aberration assay. Pakistan Journal of Biological Sciences. 2002; 5(2): 184-188.

  • Wurgler FE and Matter BE. Fundamental and Molecular Mechanisms of Mutagenesis. Mutation Research. 1998; 6(3): 484-6.

  • Stickberge MW. Experiments in genetics with drosophila. John Wiley & Sons, New York and London. 1998.


Abstract Views: 274

PDF Views: 0




  • Evaluation of Genotoxic Effect of Cashew Nut Kernel Oil

Abstract Views: 274  |  PDF Views: 0

Authors

S. G. Keshavamurthy
East Point College of Pharmacy, Bidarahalli, Virgonagar Post, Bangalore - 560 049, India
Dey Tathagata
East Point College of Pharmacy, # 147, Bidarahalli, Virgonagar Post, Bangalore - 560 049, India
V. Suresh
East Point College of Pharmacy, Bidarahalli, Virgonagar Post, Bangalore - 560 049, India
Jacob Jincy
East Point College of Pharmacy, Bidarahalli, Virgonagar Post, Bangalore - 560 049, India
C. S. Shastry
NGSM Institute of Pharmaceutical Sciences, Palneer, Mangalore - 575 005, India

Abstract


The present study has been carried out to investigate the genotoxic effect of Cashew nut kernel oil by Drosophila melanogaster (fruit fly) and on Allium cepa ischolar_main (meristamatric tip) methods. Different concentration of Cashew nut kernel oil in ethanol were selected and subjected for sterility test and dominant lethal test by using Drosophilla. For Allium cepa method, each concentration of test oil was applied for different times 12h, 24h and 48h. The obtained result indicates that the cashew nut kernel oil had the ability to cause genotoxic effect at 0.03% v/v concentration in ethanol and also cause different mitotic changes. These various changes appeared in depending on the duration of treatment. The results obtained from both sterility test and dominant lethal test found that Cashew nut kernel oil increases the lethality in flies while the concentration of oil increases.

Keywords


Alliumcepa, Cashew Nut Kernel Oil, Drosophila, Chromosome Abnormalities.

References