Research Journal of Pharmacology and Pharmacodynamics

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Evaluation of Effect of Rotenone on C6 Glial Cells by Comet Assay


Affiliations
1 Dept. of Pharmacognosy, SRM University Tamil Nadu, India
2 Department of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan, India
     

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Present study was performed to explore the action of rotenone on C6 glial cells. Rotenone is environmental pesticide and could specifically cause damage to dopaminergic neurons. It is well often used to make experimental models of Parkinsons disease. We have explored the effect of rotenone on glial cells at different concentrations and time points. Two time points were selected prior to their doubling time. Different cytotoxic parameters were done to investigate the effect of rotenone on cell viability. Significant decrease in cell viability was observed at increasing concentration of rotenone in time dependent manner. We performed single cell gel electrophoresis (comet assay) to investigate the DNA damage after rotenone treatment. Results found showed rotenone caused apoptotic death of glial cells in In Vitro. Our study could contribute in the concept of glia degeneration and helps in further explorations of better therapeutics for neurodegenerative diseases.
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  • Evaluation of Effect of Rotenone on C6 Glial Cells by Comet Assay

Abstract Views: 251  |  PDF Views: 0

Authors

B. Sangamewaran
Dept. of Pharmacognosy, SRM University Tamil Nadu, India
Manmeet Singh Saluja
Department of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan, India
Ajay Sharma
Department of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan, India
Chetan Dubey
Department of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan, India

Abstract


Present study was performed to explore the action of rotenone on C6 glial cells. Rotenone is environmental pesticide and could specifically cause damage to dopaminergic neurons. It is well often used to make experimental models of Parkinsons disease. We have explored the effect of rotenone on glial cells at different concentrations and time points. Two time points were selected prior to their doubling time. Different cytotoxic parameters were done to investigate the effect of rotenone on cell viability. Significant decrease in cell viability was observed at increasing concentration of rotenone in time dependent manner. We performed single cell gel electrophoresis (comet assay) to investigate the DNA damage after rotenone treatment. Results found showed rotenone caused apoptotic death of glial cells in In Vitro. Our study could contribute in the concept of glia degeneration and helps in further explorations of better therapeutics for neurodegenerative diseases.

References