Research Journal of Pharmacology and Pharmacodynamics

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Neuropharmacological Profile of Aqueous and Ethanolic Extract of Pithecellobium dulce Benth Leaves in Mice


Affiliations
1 Department of Pharmacology, Tatyasaheb Kore College of Pharmacy, A/P: Warananagar - 416113, Tal: Panhala, Dist: Kolhapur, Maharashtra, India
2 Tatyasaheb Kore College of Pharmacy, Warananagar - 416113, Maharashtra, India
     

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The present study was undertaken to evaluate several neuropharmacological activities of the aqueous and ethanolic extracts of Pithecellobium dulce Benth (Leguminosae) leaves in swiss albino mice. A crude extracts were given orally and its effects were tested on spontaneous motor activity, motor coordination, sodium pentobarbital-induced hypnosis and pentylenetetrazole (PTZ)-induced convulsions. The leaves of plant were extracted with water and ethanol by maceration and soxhelation respectively. Preliminary phytochemical screening of both extracts was carried out to determine the nature of phytoconstituents present in it. Acute toxicity studies of the both extracts were performed using mice and dose of 200 mg/kg was selected for further studies. Results from experimental models showed prominent decrease in locomotor activity and motor coordination for both extracts. In sodium pentobarbital-induced hypnosis both extracts exhibited significant hypnotic effect. Results of anticonvulsant activity testing by PTZ method revealed that aqueous and ethanolic extract of Pithecellobium dulce could not decreased the convulsions as well as mortality in mice. In conclusion, this study demonstrates that an acutely administered single dose of an aqueous and ethanolic extracts of Pithecellobium dulce leaves possess skeletal muscle relaxation activity and can exert depressant effects on the CNS but no anticonvulsant action.

Keywords

Pithecellobium dulce, Neuropharmacology, Depressant, Hypnotic.
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  • Kirtikar KR and Basu BD. Indian medicinal plants Vol. II. International Book Distributors, Dehradun. 1975.

  • Sugumaran M et al. Antidiabetic potential of aqueous and alcoholic leaf extracts of Pithecellobium dulce. Asian J of Research in Chem. 2009; 2(1): 83-85.

  • Zapesochnaya GG et al. Flavonoids of the leaves of Pithecellobium dulce. Khim Prir Soedin. 1980; 2:252.

  • Megala J and Geetha A. Free radical-scavenging and H+, K+- ATPase inhibition activities of Pithecellobium. Food Chemistry. 2010; 121:1120-1128.

  • Khare CP. Indian medicinal plants, an illustrated dictionary. Springer (India) Private Limited, New Delhi. 2007.

  • Murugesansugumaran. Pithecellobium Dulce Benth - A Review. Pharmainfo.net. 2008; 6(3). Available from: URL: http://www.pharmainfo.net/reviews/ pithecellobium-dulcebenth-review.

  • Shanmugakumaran SD et al. Pharmacognostical, antibacterial and antifungal potentials of the leaf extracts of Pithecellobium dulce Benth. Phcog Mag. 2006; 7(2): 163-167.

  • Sahu NP and Mahato SB. Anti-inflammatory triterpene saponins of Pithecellobium dulce: Characterization of an echinocystic acid bisdesmoside. The International J Plant Biochem. 1994; 37(5): 1425-1427.

  • Ghosh MN. Fundamentals of experimental pharmacology. Scientific book agency, Calcutta.1984.

  • Kulkarni SK. Hand book of experimental pharmacology. Vallabh Prakashan, New Delhi. 1999.

  • Kasture SB. A handbook of experiments in pre-clinical pharmacology. Career publication, Nasik, 2006.

  • Carlini EA et al. Pharmacology of lemongrass (Cymbopogon citratus Stapf) I. Effects of teas prepared from the leaves on laboratory animals. Journal of Ethnopharmacology. 1986; 17:37-64.

  • Speroni E and Minghetti A. Neuropharmacology activity of extracts from Passiflora incarnata. Planta Medica. 1988; 54:488-491.

  • Leewanich P et al. Behavioural studies on alkaloids extracted from leaves of Hunteria zeylanica. Biological and Pharmaceutical Bulletin. 1996; 19:394-399.

  • Nagarjun NS et al. CNS depressant activity of Dalbergia malaberica. Indian Drugs. 2003; 40:716-717.

  • Carpendo R et al. Inhibitors of kynurenrie hydroxylase and kynureniase increase cerebral formation of kynurenate and have sedative and anti-convulsant activities. Neuroscience. 1994; 61:237-243.

  • Gouemo PN et al. Some neuropharmacological effects of an ethanolic extract of Maprounea africana in rodents. Journal of Ethnopharmacology. 1994; 62:57-263.

  • Kaul PN and Kulkarni SK. New drug metabolism inhibitor of marine origin. Journal Pharmaceutical Sciences. 1978; 67:1293-1296.

  • Fujimori H. Potentiation of barbital hypnosis as an evaluation method for central nervous system depressant. Psychopharmacology. 1965; 7:374-377.

  • Petty F. GABA and mood disorders: a brief review and hypothesis. Journal of Affective Disorders 1995; 34:275-281.

  • Swinyard EA et al. Experimental selection, quantification and evaluation of anticonvulsants. In: Levy, R., Mattson, R., Meldrum, B.S., Penry, J.K., Dreifuss, F.E. (Eds.), Antiepileptic Drugs. Raven Press, New York. 1989; 3rd ed: pp. 85-102.

  • Ramanjaneyulu R and Ticku MK. Interactions of pentamethylenetetrazole and tetrazole analogues with the picrotoxinin site of the benzodiazepine-GABA receptor ionophore complex. Eur Journal of Pharmacol. 1984; 98:337-345.


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  • Neuropharmacological Profile of Aqueous and Ethanolic Extract of Pithecellobium dulce Benth Leaves in Mice

Abstract Views: 333  |  PDF Views: 2

Authors

V. S. Mule
Department of Pharmacology, Tatyasaheb Kore College of Pharmacy, A/P: Warananagar - 416113, Tal: Panhala, Dist: Kolhapur, Maharashtra, India
V. H. Potdar
Tatyasaheb Kore College of Pharmacy, Warananagar - 416113, Maharashtra, India
S. D. Jadhav
Tatyasaheb Kore College of Pharmacy, Warananagar - 416113, Maharashtra, India
J. I. Disouza
Tatyasaheb Kore College of Pharmacy, Warananagar - 416113, Maharashtra, India

Abstract


The present study was undertaken to evaluate several neuropharmacological activities of the aqueous and ethanolic extracts of Pithecellobium dulce Benth (Leguminosae) leaves in swiss albino mice. A crude extracts were given orally and its effects were tested on spontaneous motor activity, motor coordination, sodium pentobarbital-induced hypnosis and pentylenetetrazole (PTZ)-induced convulsions. The leaves of plant were extracted with water and ethanol by maceration and soxhelation respectively. Preliminary phytochemical screening of both extracts was carried out to determine the nature of phytoconstituents present in it. Acute toxicity studies of the both extracts were performed using mice and dose of 200 mg/kg was selected for further studies. Results from experimental models showed prominent decrease in locomotor activity and motor coordination for both extracts. In sodium pentobarbital-induced hypnosis both extracts exhibited significant hypnotic effect. Results of anticonvulsant activity testing by PTZ method revealed that aqueous and ethanolic extract of Pithecellobium dulce could not decreased the convulsions as well as mortality in mice. In conclusion, this study demonstrates that an acutely administered single dose of an aqueous and ethanolic extracts of Pithecellobium dulce leaves possess skeletal muscle relaxation activity and can exert depressant effects on the CNS but no anticonvulsant action.

Keywords


Pithecellobium dulce, Neuropharmacology, Depressant, Hypnotic.

References