Research Journal of Pharmacology and Pharmacodynamics

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Toxicological Studies of Aqueous Leaf Extract of Mucuna pruriens on Albino Rats


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1 Department of Biochemistry, University of Nigeria, Nsukka, India
     

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The leaves of Mucuna pruriens have been used traditionally for the treatment of various ailments but little information has been documented about its toxicological studies of the aqueous leaf extract of Mucuna pruriens on albino rats to ascertain its hepatotoxic effect when used in traditionally medicine. Preliminary phytochemical analysis was carried out by the method of Harboune (1973). The ALT and AST concentrations were measured using Human test kits (Germany). Total protein and bilirubin were measured using standard biochemical methods as described in Amadi et al. 2004. The phytochemical analysis indicated the presence of flavonoids, tannins, terpenoids, alkaloids, saponins and trace amounts of cyanogenic glycosides. The leaf extract of graded doses of 400 and 800mg/kg body weight given orally to the rats caused no significant increase or decrease (p>0.05) in the serum ALT, AST, total protein and bilirubin concentrations when compared to the controls. From the results of our study, we therefore conclude that the aqueous leaf extract of Mucuna pruriens had no hepatotoxic effect at the investigated doses and thus can be used in traditional medicine. However, further research needs to be carried out on higher doses and duration.

Keywords

Toxicology, Mucuna pruriens, Hepatotoxic, Phytochemistry.
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  • Amadi, B.A., Agomuo, E.N. and Ibegbulem, C.O. (2004). Research methods in Biochemistry. Supreme Publisher, Owerri, Nigeria.

  • Duke, J. (1992). Handbook of Biological Active Phytochemicals and their activities. CRR Press pp 99-131.

  • Enechi, O. C. and Odonwodo, J.C. (2003). An Assessment of the phytochemical and nutrient composition of the pulverized ischolar_main of cissus quadrangularis. Journal of Bio. Res. and Biotech., 1(1):63-68.

  • Harboune, J.B. (1973). Phytochemical methods, Chapman and Hall London 1st Ed. p288.

  • Havel, E. (2004). Liver functions. Retrieved on July 4th 2006.

  • Oduhia, P. (2001). Kapikachu or Lowhage (mucuna pruriens). Society for parthenium Management, India.

  • Okwu, D.E. and Omodamiro, O.D. (2005). Effect of Hexane extract and phytochemical content of Xylopia aethiopica and ocimum gratissimum on the uterus of Guinea pig. Bio Research, 3(2): 40-44.

  • Taylor, L.N. (2005). The Stealing Power of Rain Forest Herbs: A Guide to understanding and using Herbal Medicine. Garden City Park. N.Y.

  • Warrier, D. (1995). Variation of L-Dopa contents in mucuna species. Journal of Medicinal and Aromatic Plant Science (a). Chemistry of Herbal Drugs, Laboratory, National Botanical Research Institute, Lucknow, India, June 21(2):343-346.

  • WHO (2003). Traditional Medicine. P 4.

  • William, C.E. (2004). Trease and Evans Pharmacognosy (15th Ed). Elsevier Ltd pp 447-482.


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  • Toxicological Studies of Aqueous Leaf Extract of Mucuna pruriens on Albino Rats

Abstract Views: 252  |  PDF Views: 2

Authors

Osmond C. Enechi
Department of Biochemistry, University of Nigeria, Nsukka, India
N. Tufon Emmanuel
Department of Biochemistry, University of Nigeria, Nsukka, India
Victor N. Ogugua
Department of Biochemistry, University of Nigeria, Nsukka, India

Abstract


The leaves of Mucuna pruriens have been used traditionally for the treatment of various ailments but little information has been documented about its toxicological studies of the aqueous leaf extract of Mucuna pruriens on albino rats to ascertain its hepatotoxic effect when used in traditionally medicine. Preliminary phytochemical analysis was carried out by the method of Harboune (1973). The ALT and AST concentrations were measured using Human test kits (Germany). Total protein and bilirubin were measured using standard biochemical methods as described in Amadi et al. 2004. The phytochemical analysis indicated the presence of flavonoids, tannins, terpenoids, alkaloids, saponins and trace amounts of cyanogenic glycosides. The leaf extract of graded doses of 400 and 800mg/kg body weight given orally to the rats caused no significant increase or decrease (p>0.05) in the serum ALT, AST, total protein and bilirubin concentrations when compared to the controls. From the results of our study, we therefore conclude that the aqueous leaf extract of Mucuna pruriens had no hepatotoxic effect at the investigated doses and thus can be used in traditional medicine. However, further research needs to be carried out on higher doses and duration.

Keywords


Toxicology, Mucuna pruriens, Hepatotoxic, Phytochemistry.

References