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Wound Healing Effects of Mussaenda frondosa Extracts on Second Degree Superficial Burned Rat


Affiliations
1 Dept of Pharmacognosy, Maratha Mandal's College of Pharmacy, Belgaum, 560010, Karnataka, India
2 Dept of Pharmaceutics, Government College of Pharmacy, Bengaluru, India
3 Dept of Pharmacology, Maratha Mandal's College of Pharmacy, Belgaum, India
     

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Background: Finding more efficient agents with fewer side effects for treatment of burns has always been a concern for researchers. Silver sulfadiazine (SSD), apparently due to its antimicrobial effect, is still one of the most common prescribed agents. Previous studies suggested that leaves of Mussaenda frondosa Linn (MF) has shown antimicrobial and antiinflammatory activities. This study investigates the healing effect of MF extract in comparison with SSD in second degree burn wounds.

Methods: Adult albino rats of weight around 150-200 gm were divided into 4 groups. Standard second degree burn wounds were induced on the back of their necks. One group was treated with SSD; two groups were treated with alcoholic extract (AE) cream of MF at concentrations of 10% (AE10) and aqueous extract (AQE) cream of MF at concentrations of 10% (AQE10) and the control group which received no treatment. The duration of treatment was 16 days.

Results: This study revealed that AE and SSD noticeably improved reepithelization, lipid peroxde, and collagen bundle synthesis and had a noticeable effect on TBAR compared with the control group.


Keywords

Mussaenda frondosa Linn, Silver Sulfadiazine, Burn Rat.
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  • Medline Plus. "Burns". Retrieved 2010-09-22

  • WebMD (January 7, 2009). "Burns-Topic Overview". Firstaid andemergencies. Retrieved 2010-09-22

  • Total Burn Care, 3rd Edition, Edited by David Herndon, Saunders, 2007.

  • Radek KA, Matthies AM, Burns AL, Herinrich SA, Kovacs EJ, Dipietro LA (2005). Acute ethanol exposure impair angiogenesis and the proliferative phase of wound healing. Am. J. Physiol., 289(3): H1084-90.

  • Singer AJ, Clark RA (1999). Cutaneus wound healing. N. Engl. J. Med., 341(10): 738-46.

  • Senger DR, Ledbetter SR, Claffey KP, Papadopoulos-Sergiou A, Peruzzi CA, Detar M (1996). Stimulation of endothelial growth factor through cooperative mechanism involving the alphabeta3 integrin, osteopontin, and thrombin. Am. J. Pathol., 149(1): 293-305.

  • Leung DW, Cachianes G, Kuang WJ, Goeddel DV, Ferrara N (1989). Vascular endothelial growth factor is a secreted angiogenic mitogen. Sci., 246(4935): 1306-09.

  • Spyridopoulos I, Brogi E, Kearney M, Sullivan AB, Cetrulo C, Isner JM, Losordo DW (1997). Vascular endothelial growth factor inhibits endothelial cell aptosis induced by tumor necrosis factor-alpha: balance between growth and death signals. J. Mol. Cell Cardiol., 29(5): 1321-30.

  • Wilgus TA, Matthies AM, Radek KA, Dovi JV, Burns AL, Shankar K, DiPietro LA (2005). Novel function for vascular endothelial growth factor receptor-1 on epidermal keratinocytes. Am. J. Pathol., 167(5): 1257-66.

  • J. L. Gupta, L. K. Makhija, and S. P. Bajaj. National programme for prevention of burn injuries. Indian J Plast Surg. 2010 September; 43(Suppl): S6-S10.

  • http://www.springerlink.com/content/m627322 p 542 97782/

  • Wealth of India A Dictionary of Raw material and Industrial products Vol. III, New Delhi, Publication and Information Directorate , CSIR 1952:168.

  • Basu, B.D., Kirtikar. K. Indian Medicinal Plants. 2nd ed. International book distributors, Dehradun.1987; (2):1270 -71.

  • Jain S.K. 1991, Dictionary of Indian folk medicine and Ethnobotany, Deep publication.:83.

  • Ashok Seth. The herbs of Ayurveda. Volume III: 788.

  • Dr. Nadkarni K. M. "Indian material medica" Bombay popular prakashan; 827.

  • Meenakshisundaram sreepriya, Geetha Bali http://lib. Bioinfo.pl/medi: 67120 Mol cell Biochem. 2006 Mar, 284 (1- 2): 49-55 .

  • Khare C. P. Indian Medicinal Plants: 427.

  • Dr. Mukherji PK, Quality Control Of Herbal Drugs, 1st edition, Business Horizons, Pharmaceutical Publishers, New Delhi, 2002: 379-82.

  • Dr. Kokate CK, Practical Pharmacognosy, Vallabh Prakashan, 1994: 144.

  • Ghosh MN Fundamentals of Experimental Pharmacology. 2005:1-6.

  • Organization for economic co-operation and development, revised draft guidelines423.OECD guideline for the testing of chemicals. Revised Document. October 2000.

  • Bairy KL, Somayaji SN, Rao CM. An experimental model to produce partial thickness burn wound. Ind J Exp Biol.1997:35(1):70-2.

  • Uduppa KN. Advances in research in Indian Medicine. A dissertation submitted to Banaras Hindu University, Varanasi.1970:270-303.

  • Sharma SP, Aithal KS, Srinivasan KK. Fitoterpia.1990:61:263.

  • Okhawa H, Ohishi N, Yagi K. Annal Biochem . 1979:95:351- 55.

  • Tripathi YB, Sharma M. Indian J Biochem. Biophys.1998:35:313-16.

  • Patil PA, Kulkarni DR. Antiproliferative agents in healing of incision wounds in rats.Ind J Exp Biol.1985:23:149-50.

  • Annie Shirwaikar,Radhika Shenoy, AL Udupa, SL Udupa, Somashekar Shetty Wound healing property of ethanolic extract of leaves of Hyptis suaveoles with supportive role of antioxidant enzymes Ind J Exp Biology 2003:vol 41:238-41.

  • Madden JW, Morton D, Peacock EE. Contraction of experimental wounds in inhibiting wound contraction by using a topical smooth muscle antagonist.Surgery.1974:76(1):8-15.

  • Getie, M, T .Gebre-Mariam, R . Rietz and R. H. Neubert. 2002. Evaluation of the release profiles of flavonoids from topical formulations of the crude extract of the leaves of Dodonaea viscose (Sapindaceae). Pharmazie, 57(5): 320-22.

  • Arti Parihar, Mordhwaj S. Parihar, Stephen Milner, Satyanarayan Bhat. Oxidative stress and anti-oxidative mobilization in burn injury. Burns Volume 34, Issue 1, Feb. 2008: 6-17

  • Getie M, Gebre M, Reitz R, Neubert RH. Evaluation of the release profiles of flavonoids from topical formulations of the crude extracts of the leaves of Dodonea viscose Pharmazie.2002; 57:320-22.

  • Tsuchiya H, et.al. Comparative study on antibacterial activity of phytochemical 7-34. flavones against methicillin resistant staphylococcus aureus.J.Ethnopharmacol.1996; 50:2.


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  • Wound Healing Effects of Mussaenda frondosa Extracts on Second Degree Superficial Burned Rat

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Authors

Suhas A. Patil
Dept of Pharmacognosy, Maratha Mandal's College of Pharmacy, Belgaum, 560010, Karnataka, India
V. G. Joshi
Dept of Pharmaceutics, Government College of Pharmacy, Bengaluru, India
S. N. Sambreka
Dept of Pharmacology, Maratha Mandal's College of Pharmacy, Belgaum, India

Abstract


Background: Finding more efficient agents with fewer side effects for treatment of burns has always been a concern for researchers. Silver sulfadiazine (SSD), apparently due to its antimicrobial effect, is still one of the most common prescribed agents. Previous studies suggested that leaves of Mussaenda frondosa Linn (MF) has shown antimicrobial and antiinflammatory activities. This study investigates the healing effect of MF extract in comparison with SSD in second degree burn wounds.

Methods: Adult albino rats of weight around 150-200 gm were divided into 4 groups. Standard second degree burn wounds were induced on the back of their necks. One group was treated with SSD; two groups were treated with alcoholic extract (AE) cream of MF at concentrations of 10% (AE10) and aqueous extract (AQE) cream of MF at concentrations of 10% (AQE10) and the control group which received no treatment. The duration of treatment was 16 days.

Results: This study revealed that AE and SSD noticeably improved reepithelization, lipid peroxde, and collagen bundle synthesis and had a noticeable effect on TBAR compared with the control group.


Keywords


Mussaenda frondosa Linn, Silver Sulfadiazine, Burn Rat.

References