Research Journal of Pharmacology and Pharmacodynamics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Hepatoprotective Potentials of Butea monosperma Stem Bark Extract against Carbon Tetrachloride Induced Hepatotoxicity in Albino Rats


Affiliations
1 Pinnacle Biomedical Research Institute Bhopal, Madhya Pradesh, India
2 Smt. Vidyawati Collage of Pharmacy, Jhansi (Uttarpradesh), India
3 Gover. Girls Polytechnic College, Raipur (Chhattisgarh), India
4 Raipur Institute of Technology, Raipur (Chhattisgarh), India
5 Siksha 'O' Anusandhan University, Bhubaneswar, Orissa, India
     

   Subscribe/Renew Journal


Carbon tetrachloride (CCl4) pharmacological tool to produce liver damage in rats. Silymarin (100 mg/kg) and extract of Butea monosperma (shown to be hepatoprotective substances) prevented the CCl4 induced toxicity. Hydroalcholic extract of the stem bark of Butea Monosperma was evaluated for its hepatoprotective. This in vitro efficacy was reinforced by a significant dose dependent hepatoprotection (at 100 mg/kg and 200mg/kg dose) by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation while it significantly increased the reduced Glutathione levels of tissue in a dose dependant manner. The Hepatoprotective activities of the extract are being comparable to standards Silymarin. The results obtained in the present study indicate that stem bark extract of Butea Monosperma is a potential source of natural hepatoprotective. The hepatoprotective property may be attributed to the antioxidant potential and the phytochemical constituents of the plant. The present study justifies the claim of the native practitioner that the decoction of the plant is useful in treating jaundice and find out the clinical efficacy of the Butea Monosperma.

Keywords

Carbon Tetrachloride, Butea Monosperma, Silymarin, Alanine Amino Transferase, Glutathione, Serum Glutamate Oxaloacetate Transaminase, Serum Glutamate Pyruvate Transaminase and Hepatoprotection.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Vir Ji Chrungoo, Kuldip Singh and Jaswant Singh. Differential biochemical response of freshly isolated rat hepatocytes to paracetamol, carbon tetrochloride and d-galactosamine toxicity. Indian J Exp Bio. 35: 603-610 (1997).

  • D. Jhade, D. Ahirwar, N. K. Sharma, R. Jain and S. Gupta, Journal of Pharmacy Research, 2(7):1181-1183, (2009).

  • Kirtikar KR, Basu BD, Indian medicinal plants, Vol.II, Periodical Book agency: New Delhi, p785, (1991).

  • M.V. Patil, S. Pawar and D.A. Patil. Ethnobotany of Butea monosperma (Lam.) Kuntze in North Maharashtra, India. Nat. Prod. Rad. 5(4): 323-25 (2006).

  • K. R. Kirtikar, B.D. Basu, Indian medicinal plants, (Lalit mohan Basu, Allahabad, India, Vol. I, 2nd edition, pp. 785-88, (1935).

  • L.D. Kapoor. Handbook of Ayurvedic Medicinal Plants, Herbal Reference Library Edition (Replica Press Pvt. Ltd., India, pp.86, (2005).

  • The Wealth of India, A dictionary of India raw material and Industrial products, (Publication and Information Directorate, CSIR, New Delhi, Vol. II, pp. 1-344, (1988).

  • B.P. Ambasta. The Useful Plants of India, (Publications and Information Directorate, CSIR, New Delhi, pp. 1-91, (1994).

  • C.K. Kokate.Practical Pharmacognosy, (M/S Vallabh Prakashan, Pune, pp. 111, (1985).

  • K.M. Ko, P.K. Yick, T.W. Chiu, T.Y. Hui, C.H.K. Cheng and Y.C. Kong.Impaired antioxidant status I in CCl4 intoxicated rats: an invivo study. Fitotherapia.LXIV: 539- 544 (1993).

  • S. Retiman and A.S. Frankel. A colorimetric method for the determination of serum glutamine oxaloacetic and glutamic pyruvic transaminases. Am J of Clin Path. 28: 53-56 (1957).

  • H.T. Malloy, and K.A. Evelyn. The determination of bilirubin with the photoelectric colorimeter. J of Biol Chem. 19: 481-490 (1937).

  • L. George Ellman. Tissue Sulfydryl group. Arch of Biochem and Biop.82: 70-77(1959).

  • G. Aykae, M. Vysal, A.S. Yalein, N. Kocak-Toker, A. Sivas and H. Oz., The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicology. 36: 71-76, (1985).

  • A. John Buege and D. Steven Austein. Microsomal lipid peroxidation. (Moury Keiman Co London, pp302, (1978).

  • Wagner H, Wolff P. Natural substances with effects on the liver In: Vogel G, ed. Proceedings in life sciences - New natural products and plant drugs with pharmacological, biological or therapeutical activity. New York: Springer-Verlag Berlin. Heidelberg, 249-65, (1977).

  • Recnagel RO, Trends Pharmacol Sci, 4:129-31, (1983)

  • Indu Bala Koul and Aruna Kapil. Evaluation of the liver protective potential of Piperine, an active principle of black and long peppers. Planta Med.59: 413-417, (1993).

  • Sharma AK and Deshwal Neetu. An Overview: On Phytochemical and Pharmacological Studies of Butea Monosperma. Int.J. PharmTech Res., 3:2 (2011).


Abstract Views: 244

PDF Views: 2




  • Hepatoprotective Potentials of Butea monosperma Stem Bark Extract against Carbon Tetrachloride Induced Hepatotoxicity in Albino Rats

Abstract Views: 244  |  PDF Views: 2

Authors

Prashant Tiwari
Pinnacle Biomedical Research Institute Bhopal, Madhya Pradesh, India
Kuldeep Kumar
Smt. Vidyawati Collage of Pharmacy, Jhansi (Uttarpradesh), India
Rajnikant Panik
Gover. Girls Polytechnic College, Raipur (Chhattisgarh), India
Alok Pandey
Raipur Institute of Technology, Raipur (Chhattisgarh), India
Ashish Pandey
Raipur Institute of Technology, Raipur (Chhattisgarh), India
Pratap Kumar Sahu
Siksha 'O' Anusandhan University, Bhubaneswar, Orissa, India

Abstract


Carbon tetrachloride (CCl4) pharmacological tool to produce liver damage in rats. Silymarin (100 mg/kg) and extract of Butea monosperma (shown to be hepatoprotective substances) prevented the CCl4 induced toxicity. Hydroalcholic extract of the stem bark of Butea Monosperma was evaluated for its hepatoprotective. This in vitro efficacy was reinforced by a significant dose dependent hepatoprotection (at 100 mg/kg and 200mg/kg dose) by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation while it significantly increased the reduced Glutathione levels of tissue in a dose dependant manner. The Hepatoprotective activities of the extract are being comparable to standards Silymarin. The results obtained in the present study indicate that stem bark extract of Butea Monosperma is a potential source of natural hepatoprotective. The hepatoprotective property may be attributed to the antioxidant potential and the phytochemical constituents of the plant. The present study justifies the claim of the native practitioner that the decoction of the plant is useful in treating jaundice and find out the clinical efficacy of the Butea Monosperma.

Keywords


Carbon Tetrachloride, Butea Monosperma, Silymarin, Alanine Amino Transferase, Glutathione, Serum Glutamate Oxaloacetate Transaminase, Serum Glutamate Pyruvate Transaminase and Hepatoprotection.

References