Research Journal of Pharmacology and Pharmacodynamics

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In Vivo Anti-Snake Venom Activity of Methanol Extract of Leaves of Orthosiphon stamineus in Mice


Affiliations
1 Department of Pharmacology, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
2 Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamilnadu, India
3 Department of Pharmaceutical Chemistry, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore Tamilnadu, India
4 Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore Tamilnadu, India
     

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Aim of this study was to evaluate the in vivo anti-snake venom activity of leaves of Orthosiphon stamineus were studied against Cobra (Naja Naja) venom. The in vivo study was carried out by using Swiss albino mice in modifying the lethal effect of the test dose of the Cobra venom. In in vivo model the effectiveness of the extract was evaluated by oral administration of two different doses (200 and 400 mg/kg) of the methanolic extract of the leaves of Orthosiphon stamineus 5 minutes prior to the injection of the venom and the percentage of mortality was observed. The extract markedly decreased the percentage of mortality in venom induced toxicity in mice at the dose of 400mg/kg b.w which indicates the significant anti-snake venom activity of the plant there by justifying its use in the indigenous system of medicine. The present study has confirmed the ethnomedical use of the plant for the treatment of snake bite.

Keywords

Snake venom, Naja Naja, Orthosiphon stamineus, Leaves, Mice.
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  • In Vivo Anti-Snake Venom Activity of Methanol Extract of Leaves of Orthosiphon stamineus in Mice

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Authors

C. Maheswari
Department of Pharmacology, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
R. Venkatnarayanan
Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamilnadu, India
P. Babu
Department of Pharmaceutical Chemistry, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore Tamilnadu, India
C. S. Kanadasamy
Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore Tamilnadu, India

Abstract


Aim of this study was to evaluate the in vivo anti-snake venom activity of leaves of Orthosiphon stamineus were studied against Cobra (Naja Naja) venom. The in vivo study was carried out by using Swiss albino mice in modifying the lethal effect of the test dose of the Cobra venom. In in vivo model the effectiveness of the extract was evaluated by oral administration of two different doses (200 and 400 mg/kg) of the methanolic extract of the leaves of Orthosiphon stamineus 5 minutes prior to the injection of the venom and the percentage of mortality was observed. The extract markedly decreased the percentage of mortality in venom induced toxicity in mice at the dose of 400mg/kg b.w which indicates the significant anti-snake venom activity of the plant there by justifying its use in the indigenous system of medicine. The present study has confirmed the ethnomedical use of the plant for the treatment of snake bite.

Keywords


Snake venom, Naja Naja, Orthosiphon stamineus, Leaves, Mice.

References