Research Journal of Pharmacology and Pharmacodynamics

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Helmintholytic Activity Of the Methanolic and Aqueous Extract of Seeds of Cleome viscose


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1 Department of Pharmacognosy and Pharmacology, Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally, Yadagirigutta, Nalgonda-508286, Andhra Pradesh, India
     

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The aim of the present study was to evaluate helmintholytic activity of crude methanolic and aqueous extract of seeds of Cleome viscosa using Pheretima posthuma as test worm. Albendazole was included as standard reverence and distilled water as control. The results of present study indicated that the crude methanolic extract significantly paralysis and also caused death of worm in short time, as compared to aqueous extract. The both extract showed greater P and D time than the standard reference albendazole. Various concentrations of (25, 50, 100 mg/ml) of methanolic and aqueous extracts were tested in the bioassay, which involved the determination of time of paralysis (P) and time of death (D) of the worms. Methanolic extract shows less time (2.88 min for paralysis and 8.64 min for death of worm with 100 mg/ml of methanolic extract of Cleome vicosa) for paralysis and death of the worms as compared to the aqueous extract in the study. In conclusion, the traditional use of seeds of the plant Cleome viscosa as an helmintholytic activity have been confirmed and further studies are suggested to isolate the active principles responsible for activity. These observations showed the helmintholytic activity of Cleome viscosa seeds of methanolic and aqueous extract in paralysis and death of Pheretima posthuma.

Keywords

Albendazole, Cleome Viscosa, Helmintholytic, Pheretima Posthuma.
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  • Helmintholytic Activity Of the Methanolic and Aqueous Extract of Seeds of Cleome viscose

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Authors

Matsyagiri Lenkalapally
Department of Pharmacognosy and Pharmacology, Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally, Yadagirigutta, Nalgonda-508286, Andhra Pradesh, India
Sumathi Cheruku
Department of Pharmacognosy and Pharmacology, Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally, Yadagirigutta, Nalgonda-508286, Andhra Pradesh, India
Shravya Koppula
Department of Pharmacognosy and Pharmacology, Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally, Yadagirigutta, Nalgonda-508286, Andhra Pradesh, India
Sindhuja Sandhi
Department of Pharmacognosy and Pharmacology, Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally, Yadagirigutta, Nalgonda-508286, Andhra Pradesh, India
Narayana Mandapu
Department of Pharmacognosy and Pharmacology, Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally, Yadagirigutta, Nalgonda-508286, Andhra Pradesh, India
N. L. Gowri Shankar
Department of Pharmacognosy and Pharmacology, Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally, Yadagirigutta, Nalgonda-508286, Andhra Pradesh, India

Abstract


The aim of the present study was to evaluate helmintholytic activity of crude methanolic and aqueous extract of seeds of Cleome viscosa using Pheretima posthuma as test worm. Albendazole was included as standard reverence and distilled water as control. The results of present study indicated that the crude methanolic extract significantly paralysis and also caused death of worm in short time, as compared to aqueous extract. The both extract showed greater P and D time than the standard reference albendazole. Various concentrations of (25, 50, 100 mg/ml) of methanolic and aqueous extracts were tested in the bioassay, which involved the determination of time of paralysis (P) and time of death (D) of the worms. Methanolic extract shows less time (2.88 min for paralysis and 8.64 min for death of worm with 100 mg/ml of methanolic extract of Cleome vicosa) for paralysis and death of the worms as compared to the aqueous extract in the study. In conclusion, the traditional use of seeds of the plant Cleome viscosa as an helmintholytic activity have been confirmed and further studies are suggested to isolate the active principles responsible for activity. These observations showed the helmintholytic activity of Cleome viscosa seeds of methanolic and aqueous extract in paralysis and death of Pheretima posthuma.

Keywords


Albendazole, Cleome Viscosa, Helmintholytic, Pheretima Posthuma.

References