Research Journal of Pharmacology and Pharmacodynamics

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Cardioprotective Activity of Ashwagandharishta on Isoproterenol Induced Myocardial Infarction


Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India
2 Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, India
     

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The present study was designed to evaluate the cardio protective activity of Ashwagandharishta-T, Ashwagandahrishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Ashwagandharishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29th and 30th day. On 31st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed, hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Ashwagandharishta significantly prevented the ISO-induced adverse changes in the level of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Ashwagandharishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.

Keywords

Myocardial Infarction, Isoproterenol, Ashwagandharishta.
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  • Cardioprotective Activity of Ashwagandharishta on Isoproterenol Induced Myocardial Infarction

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Authors

Preeti Tiwari
Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India
Rakesh K. Patel
Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, India

Abstract


The present study was designed to evaluate the cardio protective activity of Ashwagandharishta-T, Ashwagandahrishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Ashwagandharishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29th and 30th day. On 31st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed, hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Ashwagandharishta significantly prevented the ISO-induced adverse changes in the level of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Ashwagandharishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.

Keywords


Myocardial Infarction, Isoproterenol, Ashwagandharishta.

References